GB/T 28982-2012 English PDFUS$369.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28982-2012: Detection of Tomato spotted wilt virus by PCR Status: Valid
Basic dataStandard ID: GB/T 28982-2012 (GB/T28982-2012)Description (Translated English): Detection of Tomato spotted wilt virus by PCR Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 16,141 Quoted Standard: SN/T 2122 Regulation (derived from): National Standards Bulletin No. 41 of 2012 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the tomato spotted ginger virus RT-PCR, RT-PCR and immunomagnetic real-time fluorescent RT-PCR detection methods. This standard applies to including seeds, seedlings and vegetative propagation material of live plant material and pa GB/T 28982-2012: Detection of Tomato spotted wilt virus by PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection of Tomato spotted wilt virus by PCR ICS 65.020.01 B16 National Standards of People's Republic of China Tomato spotted wilt virus PCR detection method Issued on. 2012-12-31 2013-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Xiamen People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. to Cui, Yang Cuiyun, Liao Furong, Yi Jianping, Tang Cong Liang, Qi Longjun. Tomato spotted wilt virus PCR detection method1 ScopeThis standard specifies the tomato spotted wilt virus, RT-PCR, MACS fluorescent RT-PCR and real-time RT-PCR detection method. This standard applies to, including seeds, seedlings living plant material and vegetative propagation material and mass poisoning mediator carrying tomato spotted wilt virus Detection.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled3 Basic information of tomato spotted wilt virusChinese name. Tomato spotted wilt virus English name. tomatospottedwiltvirus Abbreviation. TSWV Taxonomic position. Bunyaviridae (Bunyaviridae), tomato spotted wilt virus genus (Tospovirus) Tomato spotted wilt virus For additional information, see Appendix A.4 principle of the methodWith specific antibodies modified strains magnetic adsorption test sample of virus particles released by pyrolysis viral RNA, or directly from a pending Test sample material extracted from plants of total RNA, cDNA synthesis and then under the action of reverse transcriptase to cDNA as a template and then through the ordinary RT-PCR or real-time RT-PCR to detect fluorescence. PCR analysis of the results can be clear whether the material contains a virus nucleic acid components. 5 instruments, appliances and reagents 5.1 Equipment PCR, real-time PCR instrument, rotating incubator, electrophoresis, gel imaging analysis system, water bath, high-speed refrigerated centrifuge, high Pressure autoclaves, microwaves, a vortex, electronic scales (sense of volume 0.001g), ultra-low temperature freezer (-80 ℃) and so on. 5.2 Appliances Magnetic frame, adjustable micropipette, RNase-free tips, RNase-free tubes, PCR tubes, mortar and the like. 5.3 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. Reagent preparation see Appendix B. Tomato spotted wilt virus antibodies, beads (superparamagnetic polystyrene beads, the diameter of 280nm, the surface marked with tosyl), ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28982-2012_English be delivered?Answer: Upon your order, we will start to translate GB/T 28982-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 28982-2012_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 28982-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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