GB/T 28981-2012 English PDF

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GB/T 28981-2012: Detection and identification of odontoglossum ringspot virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28981-2012	279	Add to Cart	3 days	Detection and identification of odontoglossum ringspot virus	Valid

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Basic data

Standard ID: GB/T 28981-2012 (GB/T28981-2012)
Description (Translated English): Detection and identification of odontoglossum ringspot virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 12,111
Regulation (derived from): National Standards Bulletin No. 41 of 2012
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the Odontoglossum ringspot virus serology and molecular biological characteristics of the detection method. This standard applies to seeds, bulbs, flowers and seeds and other plant material Odontoglossum ringspot virus detection, b

GB/T 28981-2012: Detection and identification of odontoglossum ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of odontoglossum ringspot virus ICS 65.020.01 B16 National Standards of People's Republic of China Odontoglossum ringspot virus quarantine and identification methods Issued on. 2012-12-31 2013-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Shanghai People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine, People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters of this standard. Wen Weigang, Chen Xianfeng, Yang Cuiyun, Guo Lixin, Lu Jie, Zhang Yongjiang, Zhang Mingzhe, Xu Ying, Cuijun Xia, Zhang Huili. Odontoglossum ringspot virus quarantine and identification methods

1 Scope

This standard specifies the method for detecting biological characteristics of spot serological and molecular Odontoglossum ring. This standard is applicable to the detection of seeds, bulbs, flowers and seeds and other plant material Tooth ringspot virus, but also for aphids and other communication media Medium detected the virus.

2 Odontoglossum ringspot virus basic information

Chinese name. Odontoglossum ringspot virus Name. odontoglossumringspotvirus Abbreviation. ORSV Tobacco mosaic virus belongs to the genus (Tobamovirus), Odontoglossum ringspot virus For additional information, see Appendix A.

3 PRINCIPLE OF THE METHOD

Based on the use of antigen-antibody reaction of the double-antibody sandwich enzyme-linked immunosorbent assay (Doubleantibodysandwichenzyme-linked immunosorbentassay, DAS-ELISA), reverse transcription polymerase chain reaction in vitro DNA synthesis technology (Reverse transcriptionpolymerasechainreaction, RT-PCR), real-time fluorescence reverse transcription-polymerase chain reaction (real-time fluorescentRT-PCR) detection and identification. 4 equipment, appliances and reagents 4.1 Equipment PCR, real-time PCR instrument, clean benches, electronic balance (the amount of sense. 0.001g), electrophoresis, electrophoresis tank, gel imaging system, High-speed refrigerated centrifuge, ultra-low temperature freezer (-80 ℃), autoclaves, small-scale food processing machines, microwave ovens, microplate reader. 4.2 Appliances Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no RNase centrifuge tubes, PCR tubes and mortar and the like. 4.3 Reagents Reagents and buffers see Appendices B and C.

5 Detection

5.1 Sample Preparation Take 0.5g ~ 10g seed, bulb scales or deformity, discoloration symptoms leaves and other plant tissues, in a mortar or small food processor ......

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