GB/T 28660-2012 English PDF

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GB/T 28660-2012: Genuineness and purity verification of potato seed-tuber -- SSR molecular marker
Status: Valid

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GB/T 28660-2012	399	Add to Cart	3 days	Genuineness and purity verification of potato seed-tuber -- SSR molecular marker	Valid

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Basic data

Standard ID: GB/T 28660-2012 (GB/T28660-2012)
Description (Translated English): Genuineness and purity verification of potato seed-tuber -- SSR molecular marker
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B04
Classification of International Standard: 65.020
Word Count Estimation: 17,154
Quoted Standard: GB 18133
Regulation (derived from): National Standards Bulletin No. 24 of 2012
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the SSR molecular markers of potato (Solanum tuberosum L.) varieties approach. This standard applies to potato authenticity and purity testing.

GB/T 28660-2012: Genuineness and purity verification of potato seed-tuber -- SSR molecular marker

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Genuineness and purity verification of potato seed-tuber.SSR molecular marker ICS 65.020 B04 National Standards of People's Republic of China Seed Potato authenticity and purity identification SSR markers Issued on. 2012-09-03 2012-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee seed crops (SAC/TC37) centralized. This standard was drafted. Tuber Crops Research Institute, Yunnan Normal University, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences, the Northeast Agricultural University agronomy Institute, College of Horticulture, Huazhong Agricultural University, Heilongjiang Academy of Agricultural Sciences, Ministry of Agriculture Seed Potato Quality Supervision and Testing Center, the International Potato Center (CIP) Beijing Liaison Office. The main drafters of this standard. Li Canhui, Hao Dahai, Jin Liping, Yang Shizhong, pipe Zhaoxu, Huangsan Wen, Chen Yi, the Xie from China, Bai Yanju, Guowei. Seed Potato authenticity and purity identification SSR markers

1 Scope

This standard specifies the method of SSR markers to identify potato (Solanumtuberosum L.) cultivars. This standard applies to seed potatoes authenticity and purity testing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 18133 virus-free potato seed

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Simple sequence repeat simplesequencerepeat; SSR The basic unit of the genome of one to six nucleotides of the DNA sequence of repeated tandem configuration. 3.2 Polymerase chain reaction polymerasechainreaction; PCR In the thermostable DNA polymerase, in a large number of in vitro rapid amplification of a specific DNA sequence-specific methods.

4 instruments and reagents

4.1 instruments and appliances 4.1.1 Gradient PCR instrument. 4.1.2 low-speed centrifuge. 4.1.3 electrophoresis (meet 3000V voltage) and supporting electrophoresis tank. 4.1.4 gel imaging analysis system. 4.1.5 refrigerator (-28 ℃ ~ 4 ℃). 4.1.6 UV/Vis spectrophotometer. 4.1.7 electric heated water bath (37 ℃, 42 ℃, 65 ℃ and 95 ℃). 4.1.8 ice machine. 4.1.9 Vortex. 4.1.10 autoclave. 4.1.11 liquid nitrogen tank. 4.1.12 acidity (pH meter). 4.1.13 Shaker. ......

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