GB/T 28630.5-2012 English PDFUS$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28630.5-2012: Diagnostic protocols for white spot disease -- Part 5: T-E staining method with fresh tissue Status: Valid
Basic dataStandard ID: GB/T 28630.5-2012 (GB/T28630.5-2012)Description (Translated English): Diagnostic protocols for white spot disease -- Part 5: T-E staining method with fresh tissue Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 65.020.30 Word Count Estimation: 6,668 Quoted Standard: GB/T 28630.4-2012, Regulation (derived from): National Standards Bulletin 2012 No. 17 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the TE spot syndrome fresh tissue staining reagents and materials required, equipment, procedures, and results of determination. This section applies to diagnostic laboratories in the field or the sick dying shrimp larvae, juvenile GB/T 28630.5-2012: Diagnostic protocols for white spot disease -- Part 5: T-E staining method with fresh tissue---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. ICS65.020.30 B41 National Standards of People's Republic of China (WSD) diagnostic protocols spot syndrome Part 5. fresh tissue staining TE Part 5. T-Estainingmethodwithfreshtissue Issued on. 2012-11-01 2012-07-31 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordGB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts. --- Part 1. nucleic acid probe dot blot hybridization assay; --- Part 2. nested PCR assay; --- Part 3. In situ hybridization assay; --- Part 4. Histopathological diagnosis method; --- Part 5. TE fresh tissue staining. This is Part 5 GB/T 28630 in. Rules in this section in accordance with GB/T 1.1-2009 given draft. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li. (WSD) diagnostic protocols spot syndrome Part 5. fresh tissue staining TE1 ScopeThis section GB/T 28630 regulations required TE Spot Syndrome fresh tissue staining reagents and materials, equipment, operation Steps and results determined. This section applies to diagnostic laboratories in the field or the sick dying shrimp shrimp larvae, juvenile or adult shrimp for rapid diagnosis, it does not apply Noninfectious specimens carry latent infection or viral diagnosis.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated edition applies to this article Member. Where not dated references, the latest edition (including any amendments) applies to this document. Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method Principle 3 Pathogens and pathological features of White Spot Syndrome 3.1 See GB/T 28630.4-2012 2.1. 3.2 Technical Principle Trypan blue containing a weakly basic group and a strongly acidic group, weakly basic groups which may be combined with a nucleic acid, and strongly acidic groups exist In so weak acid and trypan blue dye eosin intermixing without precipitation occurs. Thus, a mixture of trypan blue and eosin will be able to make fresh once Tissue infected with blue nuclei, cytoplasm can be infected red.4 Reagents and materials4.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 4.2 TE Staining Solution. See Appendix A of the provisions.5 Equipment5.1 scalpel. 5.2 pointed tweezers. 5.3 medical scissors. 5.4 slide. 5.5 coverslip. 5.6 Optical Microscope. with high magnification (40 ×) and oil immersion objective (100 ×). ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28630.5-2012_English be delivered?Answer: Upon your order, we will start to translate GB/T 28630.5-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 28630.5-2012_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 28630.5-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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