GB/T 28630.4-2012 English PDF

US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 28630.4-2012: Diagnostic protocols for white spot disease -- Part 4: Histopathology method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28630.4-2012	279	Add to Cart	3 days	Diagnostic protocols for white spot disease -- Part 4: Histopathology method	Valid

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 28630.3   GB/T 28630.5   GB/T 28630.2   

Basic data

Standard ID: GB/T 28630.4-2012 (GB/T28630.4-2012)
Description (Translated English): Diagnostic protocols for white spot disease -- Part 4: Histopathology method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 12,119
Regulation (derived from): National Standards Bulletin 2012 No. 17
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the spot syndrome histopathological diagnosis reagents and materials required, equipment, procedures, and results of determination. This section applies to the onset of shrimp or other sensitive host undiagnosed and diagnosed with

GB/T 28630.4-2012: Diagnostic protocols for white spot disease -- Part 4: Histopathology method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic protocols for white spot disease Part 4. Histopathology method ICS 65.020.30 B41 National Standards of People's Republic of China (WSD) diagnostic protocols spot syndrome Part 4. Histopathological diagnosis method Part 4. Histopathologymethod Issued on. 2012-07-31 2012-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts. --- Part 1. nucleic acid probe dot blot hybridization assay; --- Part 2. nested PCR assay; --- Part 3. In situ hybridization assay; --- Part 4. Histopathological diagnosis method; --- Part 5. TE fresh tissue staining. This section GB/T Part of 428,630. This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li. (WSD) diagnostic protocols spot syndrome Part 4. Histopathological diagnosis method

1 Scope

This section GB/T 28630 specifies the necessary reagents and materials, equipment, procedures spot syndrome histopathological diagnosis method And the results of determination. This section applies to the onset of shrimp or other sensitive host confirmed and suspected infected hosts diagnosis. This section does not apply to latent infection or noninfectious virus carrying the specimens tested for the virus. Principle 2 Pathogens and pathological features of White Spot Syndrome 2.1 White Spot Syndrome (whitespotsyndrome), also known as vitiligo (whitespotdisease), white spot syndrome, shrimp fulminant epidemic disease. Spot Syndrome has acute onset and high mortality characteristics, symptoms onset stop feeding shrimp, unresponsive, or lying poolside or Floating in the water, some dying shrimp appear white patches on the carapace, sometimes accompanied by abdominal segment slightly red shrimp. White Spot Syndrome pathogen is white spot virus (whitespotsyndromevirus, WSSV) also known as white spot syndrome virus (white spotvirus, WSV), which is a double-stranded DNA (dsDNA) viruses that can cause the majority of farmed shrimp has a fatal infection. virus Oblong particles having a capsule and a unique tail-like, diameter 120nm ~ 150nm, length 270nm ~ 290nm, its genome size About 300kb. Virus replication in the nucleus, do not form a closed body (occlusionbody). Moribund shrimp showed lesions in systemic ectodermal and mesodermal origin of the tissue, the virus infects the epithelial tissue includes a lower crustaceans (including Including surface and under the skin appendages of crustaceans, gastric epithelial cells and intestinal epithelium), hematopoietic tissue, gill, connective tissue, antennal gland, blood and lymphoid organs Wait. Inclusion (inclusion) infection early in the nucleus was formed eosinophilic, with the rapid replication of the virus, causing the nuclear chromatin margination, Mast cell nucleus, basophilic inclusion bodies are getting stronger. 2.2 Technical Principle Hematoxylin - eosin (HE) staining is a conventional staining method in histology and pathology. By staining solution --- hematoxylin - eosin Were organized with the combination of different components of the cell, so that the nucleus, cytoplasm and other structures within the cell to produce a clear contrast between the blue and purple and red Color, and it can be clearly observed in various pathological changes in the structure of various organizations.

3 Reagents and materials

3.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 3.2 paraffin wax (melting point 52 ℃ ~ 54 ℃). Slice. 3.3 xylene. 3.4 ethanol. 3.5 95% ethanol. 3.6 filtered seawater. salinity of seawater 20 to 30 in the room for one week, the filter paper and 0.22μm membrane filter. 3.7-Davidson's AFA (Davidson'sAFA) fixative. See Appendix A of the provisions. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 28630.4-2012_English be delivered?

Answer: Upon your order, we will start to translate GB/T 28630.4-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 28630.4-2012_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 28630.4-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.