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GB/T 28630.3-2012 English PDF

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US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 28630.3-2012: Diagnostic protocols for white spot disease -- Part 3: In situ hybridization method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28630.3-2012	279	Add to Cart	3 days	Diagnostic protocols for white spot disease -- Part 3: In situ hybridization method	Valid

Similar standards

GB/T 25165 NY/T 1663 GB/T 20365 GB/T 28630.4 GB/T 28630.5 GB/T 28630.2

Basic data

Standard ID: GB/T 28630.3-2012 (GB/T28630.3-2012)
Description (Translated English): Diagnostic protocols for white spot disease -- Part 3: In situ hybridization method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 12,183
Quoted Standard: GB/T 28630.1-2012; GB/T 28630.4-2012
Regulation (derived from): National Standards Bulletin 2012 No. 17
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the White Spot Syndrome in situ hybridization assay reagents and materials required, equipment, procedures, and results of determination. This section applies to conduct spot syndrome of shrimp and other sensitive host tissue cells

GB/T 28630.3-2012: Diagnostic protocols for white spot disease -- Part 3: In situ hybridization method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic protocols for white spot disease Part 3. In situ hybridization method ICS 65.020.30 B41 National Standards of People's Republic of China (WSD) diagnostic protocols spot syndrome Part 3. In situ hybridization assay Part 3. Insituhybridizationmethod Issued on. 2012-07-31 2012-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts. --- Part 1. nucleic acid probe dot blot hybridization assay; --- Part 2. nested PCR assay; --- Part 3. In situ hybridization assay; --- Part 4. Histopathological diagnosis method; --- Part 5. TE fresh tissue staining. This section GB/T Part of 328,630. This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li. (WSD) diagnostic protocols spot syndrome Part 3. In situ hybridization assay

1 Scope

This section GB/T 28630 specifies the desired spot syndrome in situ hybridization assay reagents and materials, equipment, procedures, and Result judgment. Assessment This section applies to conduct spot syndrome of shrimp and other sensitive host tissue cells and virus amplification degree of infection status And diagnosis of the disease. Not suitable for carrying the virus non-infectious specimens for virus detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. Who does not note date reference documents, the latest edition (including any amendments) applies to this document. Diagnostic protocols GB/T 28630.1-2012 spot syndrome (WSD) - Part 1. nucleic acid probe dot blot hybridization assay Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method Principle 3 Pathogens and pathological features of White Spot Syndrome 3.1 See GB/T 28630.4-2012 2.1. 3.2 Technical Principle In situ hybridization method is based on a non-radioactive marker --- digoxigenin (DIG) labeled white spot syndrome virus (WSSV) nucleic acid probe Needle and slice WSSVDNA present in nucleic acid hybridization, and by an antigen - antibody reaction and enzyme - substrate chemical color reaction It shows the presence of WSSV sites on tissue sections. In situ hybridization can be kept intact tissue and cell morphology in a complex organization can single A cell for virus detection, accurately reflect the tissue distribution of the virus in cells.

4 Reagents and materials

4.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 4.2 nucleic acid probe according to GB/T 28630.1-2012 6.2 requirements. 4.3 DIG alkaline phosphatase conjugated antibody (AP- anti-DIG, 0.75U/μL, 4 ℃). chemical reagents. 4.4 xylene. 4.5 ethanol. 4.6 95% ethanol. 4.7 neutral gum. Biological staining. 4.8 paraffin wax (melting point 52 ℃ ~ 54 ℃). Slice. 4.9-Davidson's AFA (Davidson'sAFA) fixative. See Appendix A of the provisions. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 28630.3-2012_English be delivered?

Answer: Upon your order, we will start to translate GB/T 28630.3-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 28630.3-2012_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 28630.3-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.