GB/T 28630.2-2012 English PDF

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GB/T 28630.2-2012: Diagnostic protocols for white spot disease -- Part 2: Nested PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28630.2-2012	399	Add to Cart	3 days	Diagnostic protocols for white spot disease -- Part 2: Nested PCR method	Valid

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GB/T 25165   NY/T 1663   GB/T 20365   GB/T 28630.4   GB/T 28630.5   GB/T 28630.3   

Basic data

Standard ID: GB/T 28630.2-2012 (GB/T28630.2-2012)
Description (Translated English): Diagnostic protocols for white spot disease -- Part 2: Nested PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 17,130
Quoted Standard: GB/T 28630.4-2012
Regulation (derived from): National Standards Bulletin 2012 No. 17
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the spot syndrome nested PCR pathogen detection methods and materials necessary reagents, equipment, procedures, and results of determination. This section applies to a variety of shrimp samples, environmental biotechnology and foo

GB/T 28630.2-2012: Diagnostic protocols for white spot disease -- Part 2: Nested PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic protocols for white spot disease Part 2. Nested PCR method ICS 65.020.30 B41 National Standards of People's Republic of China (WSD) diagnostic protocols spot syndrome Part 2. nested PCR assay Part 2. NestedPCRmethod Issued on. 2012-07-31 2012-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts. --- Part 1. nucleic acid probe dot blot hybridization assay; --- Part 2. nested PCR assay; --- Part 3. In situ hybridization assay; --- Part 4. Histopathological diagnosis method; --- Part 5. TE fresh tissue staining. This is Part 2 GB/T 28630 of. This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li. (WSD) diagnostic protocols spot syndrome Part 2. nested PCR assay Warning. Use of this standard shall have a person experiences regular laboratory work, this standard does not point out all possible security issues. Users have the responsibility to take appropriate safety and health practices and to ensure compliance with the conditions relevant national regulations.

1 Scope

This section GB/T 28630 specifies the nested PCR for detection of White Spot Syndrome pathogen required reagents and materials, equipment, Procedure and result judgment. This section applies to a variety of samples of shrimp, a variety of biological and environmental samples of food organisms, and various other non-biological samples for White Spot Syndrome pathogens with high sensitivity qualitative detection of infected cases, for pathogen screening and diagnosis of diseases. When used alone in this section do not apply to activity estimation and evaluation of the degree of host infected with the virus or the amount of infection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. Who does not note date reference documents, the latest edition (including any amendments) applies to this document. Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method Principle 3 Pathogens and pathological features of White Spot Syndrome 3.1 See GB/T 28630.4-2012 2.1. 3.2 Technical Principle Polymerase chain reaction (polymerasechainreaction, PCR) is a DNA sample to be tested as a template, with the template A pair of specific oligonucleotide sequences complementary to both ends of the sequence as a primer, in the presence of four kinds of deoxyribonucleoside triphosphates, use-dependent DNA synthesis of DNA polymerase. After dozens of denaturation, annealing and extension reaction cycle, so that between the two primers on the template DNA fragment obtained specifically between the amplification stages, is specifically amplified fragment was detected by electrophoresis and other means, which can reveal Trace viral DNA. Nested PCR is the first step in the PCR amplified product, and then a pair of primers second step PCR amplification increase. Nested PCR reactions can be greatly increased sensitivity and specificity of PCR detection.

4 Reagents and materials

4.1 Unless otherwise stated in the analysis using only the confirmation of analytical grade chemicals and distilled or deionized water or equivalent purity. 4.2 ethanol. 4.3 liquid paraffin. 4.4 chlorine disinfectant. medical supplies or aquatic drugs. 4.5 agarose. electrophoresis grade. ......

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