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GB/T 28082-2011 English PDF

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GB/T 28082-2011: Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 28082-2011299 Add to Cart 3 days Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf. Valid

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Basic data

Standard ID: GB/T 28082-2011 (GB/T28082-2011)
Description (Translated English): Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 13,112
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the elm Fusarium were isolated and cultured, morphological identification methods. This standard applies to Fusarium oxysporum from elm countries and regions have occurred Ulmus (Ulmus L.) seedlings, timber, wood products and wood packaging elm Fusarium quarantine and identification.

GB/T 28082-2011: Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf.


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Ophiostoma novo-ulmi Brasier and Ophiostoma ulmi (Buisman) Nannf. ICS 65.020.01 B16 National Standards of People's Republic of China Elm quarantine and identification of Fusarium oxysporum andOphiostomaulmi (Buisman) Nannf. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Chinese Academy of Inspection and Quarantine. Drafters of this standard. Wu Shan goods, Yan Jin, Du Hongzhong. Elm quarantine and identification of Fusarium oxysporum

1 Scope

This standard specifies the elm wilt pathogen isolation and culture, morphological identification method. This standard applies to happen Fusarium oxysporum from elm countries Ulmus (UlmusL.) Nursery stock, logs, wood and wood packaging The elm quarantine and identification of Fusarium oxysporum f.

2 Elm Fusarium oxysporum basic information

Chinese name. Yu Fusarium. Name. Ophiostomanovo-ulmiBrasier; Ophiostomaulmi (Buisman) Nannf .. Synonyms. Ceratocystisulmi (Buism.) Moreau. Disease English name. dutchelmdisease, elmwiltdisease. Fungi industry Fungi, Ascomycota Ascomycota, Ascomycetes Ascomycetes, fecal bacteria Branch subclass Sordariomycetidae, long Ceratocystis head Ophiostomatales, Ceratocystis families Ophiostomataceae, Ceratocystis Ophiostoma. Elm Fusarium oxysporum For additional information, see Appendix A.

3 PRINCIPLE OF THE METHOD

Based on morphological and biological characteristics of asexual spores and sexual generation, as well as its characteristic symptoms caused by host detection and identification.

4 Instrument

4.1 biological microscope. 4.2 stereo microscope. 4.3 Light biological incubator. 4.4 autoclave. 4.5 Clean Benches. 4.6 electronic balance. 4.7 shaker.

5 Reagents and culture medium

5.1 Reagents Agar powder, malt extract, Oxoid malt extract, elm branches, glucose, asparagine (L-asparagine), potassium dihydrogen phosphate (KH2PO4), magnesium sulfate (MgSO4 · 7H2O), zinc sulfate (ZnSO4), ferric chloride (FeCl3), vitamin B1, vitamin B6, Actinobacillus Cycloheximide, streptomycin, penicillin. NOTE. actidione as virulent, pay attention to protection.
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