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GB/T 28065-2011 English PDF

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US$259.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 28065-2011: Biochip detection of Ceratitis capitata (Wiedemann)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28065-2011	259	Add to Cart	3 days	Biochip detection of Ceratitis capitata (Wiedemann)	Valid

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Basic data

Standard ID: GB/T 28065-2011 (GB/T28065-2011)
Description (Translated English): Biochip detection of Ceratitis capitata (Wiedemann)
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 11,171
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: SN/T 2039
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the equipment manufacturing industry in general parts of data dictionary elements registered RAE overall framework, including related maintenance organization, the technical assessment procedures and technical assessment rules. This standard applies to based on GB/T 17645 equipment manufacturing generic component data elements in the data dictionary maintenance request submission, registration agencies DMR technical review and update the data dictionary and publishing, also applies to users of the supervision of registered institutions.

GB/T 28065-2011: Biochip detection of Ceratitis capitata (Wiedemann)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Biochip detection of Ceratitis capitata (Wiedemann) ICS 65.020.01 B16 National Standards of People's Republic of China Mediterranean fruit fly biochip detection method Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Shenzhen Entry-Exit Inspection and Quarantine Beijing People's Republic of China, The Zhengyang Gene Co., Ltd. Shenzhen Inspection and Quarantine Science Research Institute. The main drafters of this standard. Yudao Jian, Li Jianguang, any wind Lu, Xu Lang, Zhou Qi, Zhang Guiming, Chen Chi-nan, Wang Wanchun, Conlin, Zhong Jianzhong, The only jade. Mediterranean fruit fly biochip detection method

1 Scope

This standard identifies the Mediterranean fruit fly Ceratitiscapitata (Wiedemann) (Diptera Diptera Tephritidae Tephritidae) Bio-chip preparation, testing and result determination method. This standard applies to the relevant trade and pest monitoring in the Mediterranean fruit fly eggs, larvae, pupae and adults of identification or identification review.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2039 Mediterranean fruit fly quarantine and identification of PCR.

3 Abbreviations

SN/T 2039 defined abbreviations applicable to this document.

4 principle of the method

Select Tephritidae DNACOI mitochondrial gene nucleotide molecule fragment labeled probes, the probes are arranged according to a pre-set solid It is scheduled for a specific carrier solid phase support. surface (such as a substrate of aldehyde) formed microarray, using reverse solid phase hybridization, labeled with Cy3 off Oxygen cytidine triphosphate (Cy3-dCTP) probe hybridization and microarray sample molecules fluorescently labeled on the realization of the anti-hybrid molecule between multiple It should be, and the sample to detect the presence of specific gene chip scanning signal through interpretation, and finally determine whether the Mediterranean fruit fly.

5 apparatus and appliances

PCR amplification, the chip spotter, microarray scanner, centrifuges, water bath, vortex, refrigerators, electronic scales, an oven, a magnetic stirrer Stirrer. Aldehyde of the substrate, a porous plate, cover sheet chips, chip partition fence, chip paste tools, wet boxes, hybridization boxes, timers, cylinders, chip racks, 2L beaker, 15mL centrifuge tubes, cylinders, pipettes and the like.

6 Reagents

6.1 insect genomic DNA extraction kit. 6.2 DNA fast purification/recovery kit. 6.3 deionized water. 6.4 50% dimethyl sulfoxide (DMSO). 6.5 0.2% sodium dodecyl sulfate (SDS). 6.6 0.2% sodium borohydride. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 28065-2011_English be delivered?

Answer: Upon your order, we will start to translate GB/T 28065-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 28065-2011_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 28065-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.