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GB/T 25878-2010 English PDF

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US$209.00 · In stock
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GB/T 25878-2010: Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 25878-2010	209	Add to Cart	3 days	Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV)	Valid

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Basic data

Standard ID: GB/T 25878-2010 (GB/T25878-2010)
Description (Translated English): Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV)
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 9,928
Date of Issue: 2011-01-10
Date of Implementation: 2011-06-01
Quoted Standard: SC/T 7202.1-2007; SC/T 7202.2-2007
Regulation (derived from): National Standard Approval Announcement 2011 No.1 (Total No.166)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the principles of infectious hypodermal and hematopoietic necrosis virus (IHHNV) polymerase chain reaction (PCR) detection method, necessary reagents and materials, operating procedures and the results of determination, instruments and equipment. This standard applies to shrimp, various non- food organisms and other biological samples of subcutaneous and qualitative detection of infectious hematopoietic necrosis virus (IHHNV) of, environmental biotechnology. Not suitable for the amount of virus or infection activity estimation and evaluation of the degree of host infection.

GB/T 25878-2010: Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Polymerase chain reaction (PCR) method for infectious hypodermal and haematopoietic necrosis virus (IHHNV) ICS 65.020.30 B41 National Standards of People's Republic of China Infectious hypodermal and hematopoietic necrosis virus (IHHNV) PCR detection method Polymerasechainreaction (PCR) methodforinfectioushypodermaland haematopoieticnecrosisvirus (IHHNV) Issued on. 2011-01-10 2011-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard and Appendix B is an informative annex. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Fisheries. This standard was drafted. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this standard. Huang Jie, Yang Bing, Zhu Ze Wen, Song Xiaoling, SUN Xi die, Zhao Hongping, Liu Li. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) PCR detection method Warning. Use of this standard shall have a person experiences regular laboratory work, this standard does not point out all possible security issues. Users have the responsibility to take appropriate safety and health practices and to ensure compliance with the conditions relevant national regulations.

1 Scope

This standard specifies the infectious hypodermal and (PCR) detection method hematopoietic necrosis virus (IHHNV) polymerase chain reaction Principle, the required reagents and materials, instruments and equipment, procedures and results of the determination. This standard applies to shrimp, environmental biotechnology, and various other non-food organisms in biological samples of infectious hypodermal and hematopoietic necrosis virus (IHHNV) qualitative detection. Not suitable for the amount of virus or infection activity estimation and evaluation of the degree of host infection.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SC/T 7202.1-2007 monodon baculovirus diagnostic procedures - Part 1. tablet microscopy SC/T 7202.2-2007 monodon baculovirus diagnostic procedures part 2. PCR assay Principle 3 3.1 infectious hypodermal and hematopoietic necrosis virus and its epidemic characteristics Infectious hypodermal and hematopoietic necrosis virus (infectioushypodermalandhaematopoieticnecrosisvirus, IHHNV) particle size of 20nm ~ 22nm, is nonenveloped icosahedron, the cesium chloride buoyant density of 1.40g/mL, containing a line Like single-stranded DNA, the length of 4.1kb, capsid consists of four molecular weight were 74kD, 47kD, 39kD, 37.5kD polypeptide composition. IHHNV can be infected worldwide shrimp, shrimps infection fine angle (Litopenaeusstylirostris) can cause acute Biography Infectious diseases and high mortality (90%), the juvenile shrimp being the most serious hazards. IHHNV infection vannamei (Litopenaeusvannamei) Can cause chronic "little incomplete syndrome" (RDS), the main effect is the prevalence of shrimp slow growth, deformity, infected shrimp survival lifelong carriers of the virus, Spread through the vertical and horizontal. 3.2 Technical Principle IHHNV polymerase chain reaction (polymerasechainreaction, PCR) is IHHNV base section 389 (b) The length of the specific DNA sequence as a template, a pair of sequence specific oligonucleotide sequence complementary to the template at both ends as primers, the four The presence of deoxyribonucleoside triphosphate species, the use of synthetic DNA-dependent DNA polymerase, after dozens of denaturation, annealing and extension Extending reaction cycle, so that between the template DNA fragment obtained between the two primers specifically amplified series, and then by means of electrophoresis detection Measured to be specifically amplified fragment, which reveals the presence of trace IHHNVDNA.

4 Reagents and materials

In addition to the primer, the positive and negative controls, in accordance with the provisions of 4.1 reagents required SC/T 7202.2-2007 in Chapter 5. 4.2 100ng/μL Primer F. 5'-CGG-AAC-ACA-ACC-CGA-CTT-TA-3 ', - 20 ℃ to save. 4.3 100ng/μL Primer R. 5'-GGC-CAA-GAC-CAA-AAT-ACG-AA-3 ', - 20 ℃ to save. 4.4 positive control known IHHNV positive DNA template tissue sample, -20 ℃ preservation. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 25878-2010_English be delivered?

Answer: Upon your order, we will start to translate GB/T 25878-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 25878-2010_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 25878-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.