GB/T 25877-2010 English PDF

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GB/T 25877-2010: Starch gel electrophoresis isozyme analysis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 25877-2010	1239	Add to Cart	8 days	Starch gel electrophoresis isozyme analysis	Valid

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Basic data

Standard ID: GB/T 25877-2010 (GB/T25877-2010)
Description (Translated English): Starch gel electrophoresis isozyme analysis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B50
Classification of International Standard: 65.150
Word Count Estimation: 56,511
Date of Issue: 1/10/2011
Date of Implementation: 6/1/2011
Quoted Standard: GB/T 18654.1-2008; GB/T 18654.2-2008
Regulation (derived from): Announcement of Newly Approved National Standards 2011 No. (No. 166 overall) 1
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies isozyme analysis of starch gel electrophoresis reagents and materials, equipment, sampling, analytical procedures and the results of the determination. This Standard is applicable to common light, sea level aquatic organisms common isozyme gel electrophoresis analysis.

GB/T 25877-2010: Starch gel electrophoresis isozyme analysis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Starch gel electrophoresis isozyme analysis ICS 65.150 B50 National Standards of People's Republic of China Starch gel electrophoresis isozyme analysis Issued on. 2011-01-10 2011-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B, Appendix C, Appendix D and Appendix E are normative appendix. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Fisheries. This standard was drafted. China Ocean University, the Yellow Sea Fisheries Research Institute. The main drafters of this standard. high Tianxiang, Zhang Yan, Han Zhiqiang, Xiao Yongding double, Zhang Hui. Starch gel electrophoresis isozyme analysis

1 Scope

This standard specifies the starch gel electrophoresis isozyme analysis reagents and materials, equipment, sampling, analysis procedures and results of the determination. This standard applies to common light, seawater aquatic organisms common horizontal gel electrophoresis analysis of isoenzymes.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 18654.1-2008 test germplasm for cultured fishes - Part 1. Inspection Rules Sampling Method. Part 2 Accession test fish GB/T 18654.2-2008 farming Principle 3 Use different isozymes different molecular size of electric charge in the electric field, the gel appears different isozymes mobility, By catalytic, staining, scanning, access to the various isozymes, the number and intensity of the absorption, by comparative analysis, determination of genetic characteristics of fish.

4 Reagents and materials

Unless otherwise indicated, all reagents were analytical grade or above water distilled or deionized water or equivalent purity. 4.1 Potato starch hydrolysis. 4.2 citric acid. 4.3 lactic acid. 4.4 ethylenediaminetetraacetic acid. 4.5 disodium edetate. 4.6 malic acid. 4.7 lactic acid lithium. 4.8 dibasic sodium phosphate. 4.9 sodium dihydrogen phosphate. 4.10 coenzyme Ⅰ. 4.11 coenzyme Ⅱ. 4.12 phenazine methyl sulfate. 4.13 nitroblue tetrazolium. 4.14 trisodium isocitrate. 4.15 6-phosphate sodium gluconate. 4.16 ethanol. 4.17 acetone. 4.18 α- naphthalene acetic acid. 4.19 α- glycerophosphate. 4.20 Glucose-6-phosphate. 4.21 sorbitol. ......

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