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GB/T 23815-2009 English PDF

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US$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 23815-2009: Protocol of the polymerase chain reaction for detecting plant components in meat
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 23815-2009	229	Add to Cart	3 days	Protocol of the polymerase chain reaction for detecting plant components in meat	Valid

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Basic data

Standard ID: GB/T 23815-2009 (GB/T23815-2009)
Description (Translated English): Protocol of the polymerase chain reaction for detecting plant components in meat
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 10,127
Date of Issue: 2009-05-27
Date of Implementation: 2009-09-01
Regulation (derived from): PRC National Standard Approval Announcement 2009 No.7 (Total No.147)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the qualitative PCR method for detection of pork products in the plant components. This standard applies to the qualitative detection of pork semi-finished and finished products made ??of plant components. The detection limit of 0. 5ng standard plant DNA.

GB/T 23815-2009: Protocol of the polymerase chain reaction for detecting plant components in meat

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the polymerase chain reaction for detecting plant components in meat ICS 67.050 X04 National Standards of People's Republic of China Pork products plant components Qualitative PCR Detection Posted 2009-05-27 2009-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by China National Institute of Standardization. This standard is drafted by. National Processed Food Quality Supervision and Inspection Center (Guangzhou), Guangzhou Municipal Quality Supervision and Testing Institute, China Shenzhen People's Republic of CIQ. The main drafters of this standard. Guoxin Dong, Deng Hong Ling, Tan Fangfang, Luo Haiying, Yuan Jie, Yu-luan, Xie Wen-chien. Pork products plant components Qualitative PCR Detection

1 Scope

This standard specifies the qualitative PCR method for detection of plant components pork products. This standard applies to pork from the qualitative detection of semi-finished and finished products made of plant components. The standard detection limit of 0.5ng plant DNA.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 19495.1 GMO testing - General requirements and definitions GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.7 GMO detection methods of sampling and sample preparation

3 Terms and Definitions

Terms and definitions GB/T 19495.1 established apply to this standard. 4 anti-pollution measures Measures to prevent cross-contamination detection process carried out according to GB/T 19495.2 provisions.

5 Sampling and sample preparation

Execution method according to GB/T 19495.7 requirements.

6 Determination

6.1 Principle After extraction of the sample DNA, primers were designed for plant-specific genes by PCR technology, plant gene specific amplification DNA fragments, according to the results of PCR amplification, the determination whether the sample contains plant components. 6.2 Reagents and materials Unless otherwise specified, the reagents used were of analytical grade or biochemical reagents, water as a water according to GB/T 6682 regulations. 6.2.1 primer. primer and pork products information detection of endogenous and exogenous genes in Table 1. Table 1 pork products, primer information required exogenous gene Detection of gene primer sequences PCR product size / bp Gene nature Scope tRNALeu Positive. 5'-CGAAATCGGTAGACGCTACG-3 ' Anti-. 5'-TTCCATTGAGTCTCTGCACCT-3 ' 193 plant chloroplast pork products mitochondrion 5'-AAGTTAGAGATCGGGAGCCTAA-3 ' 5'-AAGGTGACAATAGGTAGTCC-3 ' 264 porcine endogenous mitochondrial genes pork products ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 23815-2009_English be delivered?

Answer: Upon your order, we will start to translate GB/T 23815-2009_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 23815-2009_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 23815-2009_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.