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Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 23197-2022: Diagnostic techniques for avian infectious bronchitis Status: Valid GB/T 23197: Historical versions
Basic dataStandard ID: GB/T 23197-2022 (GB/T23197-2022)Description (Translated English): Diagnostic techniques for avian infectious bronchitis Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 22,266 Date of Issue: 2022-12-30 Date of Implementation: 2023-07-01 Older Standard (superseded by this standard): GB/T 23197-2008 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 23197-2022: Diagnostic techniques for avian infectious bronchitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.ICS 11.220 CCSB41 National Standards of People's Republic of China Replacing GB/T 23197-2008 Diagnosis Technique of Chicken Infectious Bronchitis Posted on 2022-12-30 2023-07-01 implementation State Administration for Market Regulation Released by the National Standardization Management Committee table of contentsPreface I Introduction II 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Clinical Diagnosis 2 6 Sample collection, storage and transportation 2 7 Virus isolation and identification 3 8 Viral RT-PCR 4 9 Viral real-time fluorescent RT-PCR 5 10 Virus genotype identification 6 11 Hemagglutination and hemagglutination inhibition test 7 12 Tracheal ring tissue culture serum neutralization test 9 13 comprehensive judgment 10 Appendix A (Normative) Preparation of common reagent solutions 12 Appendix B (informative) Preparation of RT-PCR solution 14 Appendix C (informative) Refer to Figure 16 for RT-PCR detection results of chicken infectious bronchitis virus Appendix D (informative) Refer to Figure 17 for the real-time fluorescent RT-PCR detection results of chicken infectious bronchitis virus Appendix E (informative) Calculate TOC-ID50 and serum neutralization titer by Reed-Muench method 18forewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. This document replaces GB/T 23197-2008 "Diagnostic Techniques for Chicken Infectious Bronchitis". Compared with GB/T 23197-2008, except In addition to structural adjustments and editorial changes, the main technical changes are as follows. ---Changed "clinical diagnosis" (see Chapter 5, Chapter 2 of the.2008 edition); --- Added "sample collection" and "sample processing" (see 6.3 and 6.5); ---Changed the "virus isolation and identification" (see Chapter 7, Chapter 3 of the.2008 edition); ---Changed the "viral RT-PCR" and updated the detection primers (see 8.3, Chapter 4 of the.2008 edition); --- Added "viral real-time fluorescent RT-PCR" (see Chapter 9); --- Increased "virus genotype identification" (see Chapter 10); ---Changed the "blood coagulation and hemagglutination inhibition test" (see Chapter 11, Chapter 5 of the.2008 edition); ---Changed the "tracheal ring tissue culture serum neutralization test" (see Chapter 12, Chapter 6 of the.2008 edition). Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This document was drafted by. China Animal Health and Epidemiology Center, Qingdao Agricultural University, Harbin Veterinary Research, Chinese Academy of Agricultural Sciences Institute, Linqu County Animal Husbandry Development Center. The main drafters of this document. Gong Zhenhua, Wang Jianlin, Wang Kai, Wang Kaicheng, Mou Ruiying, Wang Yanbo, Zhang Yunxiang, Wang Xiaoying, Wang Liping, Wang Suchun, Su Hong, Pang Sufen, Liu Kun, Tan Liugang, Han Zongxi, Wu Yangong, Liu Shengwang. The release status of previous versions of this document and the documents it replaces are as follows. ---It was first published as GB/T 23197-2008 in.2008; --- This is the first revision.IntroductionVirus, IBV) is an acute, highly contagious infectious disease of chickens. IB can occur in chickens of various ages, chickens less than 5 weeks old Especially susceptible, and the symptoms are more obvious. The clinical features of IB are mainly manifested as dyspnea, rales, coughing and sneezing; decreased egg production. Low, egg quality declines; chicks appear to die. IB is widely prevalent all over the world and seriously endangers the production of chicken industry. IBV belongs to Coronaviridae, Coronaviridae, and Gammavirus in classification, and is a single-stranded positive-sense RNA virus whose RNA is easily In terms of variation, IBV has diversity. Most IBV strains can cause specific lesions in the trachea, but some strains can cause kidney lesions and Genital tract lesions. At present, the IBV strains that have been isolated at home and abroad belong to more than 30 serotypes, and the cross-immunity between IBV strains of each serotype Low protection. The diagnosis of IB is mainly carried out by etiological and serological tests. Due to the widespread use of IBV live strain vaccines in production to prevent this disease, this is Clinically, it increases the difficulty of diagnosing IB. This document standardizes the clinical diagnosis technology of IB; standardizes the pathogenic diagnosis technology of IB, including virus isolation and identification, virus RT- PCR, virus real-time fluorescent RT-PCR, virus genotype identification; standardized IB serological diagnostic techniques, including hemagglutination and hemagglutination inhibition tests Test, tracheal ring tissue culture serum neutralization test. These diagnostic techniques are also recommended in the OIE Manual of Terrestrial Animal Diagnostic Tests and Vaccines. In this revision process, references were made to the relevant diagnostic techniques recommended in this manual. Diagnosis Technique of Chicken Infectious Bronchitis1 ScopeThis document specifies the clinical diagnosis, virus isolation and identification, virus RT-PCR, and virus real-time fluorescence of chicken infectious bronchitis (IB). Technical requirements for RT-PCR, virus genotype identification, hemagglutination and hemagglutination inhibition test, tracheal ring tissue culture serum neutralization test. This document is applicable to the diagnosis, quarantine, surveillance and epidemiological investigation of IB.2 Normative referencesThe content in the following documents constitutes the essential provisions of this document through normative references in the text, among which, the dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB 19489 General Requirements for Laboratory Biosafety3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 chicken infectious bronchitis Avian infectious bronchitis virus that mainly infects chickens caused by avian infectious bronchitis virus belonging to the Coronaviridae, Coronaviridae, Gammavirus genus disease. 3.2 hemagglutinin The process of agglutinating chicken erythrocytes with the antigen obtained after the culture solution of the IBV strain is digested with trypsin. Note. By selecting appropriate IBV strains and preparing IBV hemagglutination antigens, the serological diagnosis of IB is carried out. 3.3 The IB antibody specifically attaches to the specific site of the IBV hemagglutination antigen and interferes with the binding between the IBV hemagglutination antigen and the red blood cell receptor. Together, inhibit the ability of IBV hemagglutination antigen to agglutinate chicken red blood cells. 3.4 Ct value cyclethreshold The number of cycles it takes for the fluorescent signal amount in each real-time fluorescent RT-PCR reaction tube to reach the set threshold.4 AbbreviationsThe following abbreviations apply to this document. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 23197-2022_English be delivered?Answer: Upon your order, we will start to translate GB/T 23197-2022_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 23197-2022_English with my colleagues?Answer: Yes. 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