GB/T 22262-2025 English PDF

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GB/T 22262-2025: Determination of clopidol in feeds
Status: Valid

GB/T 22262: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22262-2025	279	Add to Cart	3 days	Determination of clopidol in feeds	Valid
GB/T 22262-2008	139	Add to Cart	3 days	Determination of clopidol in feed -- High-performance liquid chromatography	Valid

Similar standards

GB/T 51033   GB/T 50082   GB/T 22262   

Basic data

Standard ID: GB/T 22262-2025 (GB/T22262-2025)
Description (Translated English): Determination of clopidol in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 14,117
Date of Issue: 2025-08-01
Date of Implementation: 2026-02-01
Older Standard (superseded by this standard): GB/T 22262-2008
Issuing agency(ies): State Administration for Market Regulation, Standardization Administration of China

GB/T 22262-2025: Determination of clopidol in feeds

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT22262-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 22262-2008 Determination of Clopidogrel in Feed Released on August 1, 2025 Implementation on February 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 22262-2008 "Determination of clopidogrel in feed - High performance liquid chromatography method" and GB/T 22262- Compared with.2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2008 edition); b) The principle of high performance liquid chromatography has been modified (see 4.1, Chapter 3 of the.2008 edition); c) The experimental steps of high performance liquid chromatography have been changed (see 4.5, Chapter 7 of the.2008 edition); d) The data processing for high performance liquid chromatography (HPLC) has been modified (see 4.6, 7.2.3 of the.2008 edition); e) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 5). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Institute of Agricultural Quality Standards and Monitoring Technology, Guangdong Academy of Agricultural Sciences, Guangdong Agricultural Science Monitoring Technology Co., Ltd. Department of Agricultural Science, Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences. The main drafters of this document are. Wang Weili, Wan Kai, Lin Xuexian, Wang Xu, Deng Yiqun, Fan Xia, Pan Fei, Suo Decheng, Liu Xiaolu, Ji Tianrong, Ding Chenhong, Xiao Zemiao, Chen Zhiting, Cui Zefeng, and Tan Shuzhen. The previous versions of this document and the documents it replaces are as follows. ---First published in.2008 as GB/T 22262-2008; ---This is the first revision. Determination of Clopidogrel in Feed

1 Scope

This document describes the determination of clopidogrel in feeds by high performance liquid chromatography and liquid chromatography-tandem mass spectrometry. This document applies to compound feeds, concentrated feeds, concentrate supplements, additive premixes, mixed feed additives and Determination of clopidogrel in feed ingredients. The detection limit of the HPLC method in this document is 0.5 mg/kg, the quantification limit is 1 mg/kg, and the detection limit of the HPLC-MS/MS method is The limit of quantification is 0.1 mg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document. 4 High-performance liquid chromatography 4.1 Principle The chloramphenicol in the sample was extracted with ammonia methanol, purified by alkaline alumina solid phase extraction column, and determined by high performance liquid chromatography using external standard method. Quantitative. 4.2 Reagents or materials Warning. The organic reagents used in the test are irritating to the upper respiratory tract. For safety reasons, protective measures should be taken during operation and the operation should be carried out in a fume hood. Unless otherwise specified, only analytical grade reagents were used. 4.2.1 Water. GB/T 6682, Grade 1. 4.2.2 Methanol. chromatographic grade. 4.2.3 n-Hexane. 4.2.4 0.2% ammonia methanol solution. Accurately pipette 2 mL of ammonia, dilute to 1000 mL with methanol, and mix thoroughly. 4.2.5 Methanol solution. Measure 70 mL of methanol (4.2.2) and 30 mL of water and mix well. 4.2.6 Standard stock solution (200 μg/mL). Accurately weigh clopidogrel standard (CAS No. 2971-90-6, purity not less than 98%) 10 mg (accurate to 0.01 mg) was placed in a 50 mL brown volumetric flask, and dissolved with methanol (4.2.2) by ultrasonication, fixed to volume, and mixed. Store below -18 °C. Store for 6 months. Or purchase a certified standard solution. 4.2.7 Standard series solution. Accurately pipette an appropriate amount of standard stock solution (4.2.6), dilute to volume with methanol solution (4.2.5), mix well, and prepare ......

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