GB/T 22249-2024 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 22249-2024: Determination of lycopene in health foods Status: Valid GB/T 22249: Historical versions
Basic dataStandard ID: GB/T 22249-2024 (GB/T22249-2024)Description (Translated English): Determination of lycopene in health foods Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: X83 Classification of International Standard: 67.050 Word Count Estimation: 14,160 Date of Issue: 2024-12-31 Date of Implementation: 2025-07-01 Older Standard (superseded by this standard): GB/T 22249-2008 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 22249-2024: Determination of lycopene in health foods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.ICS 67.050 CCSX83 National Standard of the People's Republic of China Replace GB/T 22249-2008 Determination of Lycopene in Health Food Released on 2024-12-31 2025-07-01 Implementation State Administration for Market Regulation The National Standardization Administration issued ForewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document specifies the technical requirements related to food quality. For food safety requirements, please refer to relevant laws, regulations, policies and food safety standards. document. This document replaces GB/T 22249-2008 "Determination of lycopene in health foods". In addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The sample preparation and processing methods have been changed (see 7.1, 7.2, 5.1 of the.2008 edition); b) The chromatographic reference conditions have been changed (see 7.3, 5.3 of the.2008 edition); c) The detection limit and quantification limit have been changed (see Chapter 10, Chapter 1 of the.2008 edition); d) Added the calibration method for lycopene standard stock solution (see Appendix A). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed and coordinated by the National Technical Committee for Standardization of Special Foods (SAC/TC466). This document was drafted by. China Light Industry Technology Innovation Center Co., Ltd., Zhejiang Provincial Institute of Food and Drug Inspection, China Food Fermentation Industry Research Institute Institute Co., Ltd., Chenguang Biotechnology Group Co., Ltd., Nanjing Zhongke Pharmaceutical Co., Ltd., Xianle Health Technology (Guangdong) Co., Ltd. Infinitus (China) Co., Ltd., Guangdong Changxing Biotechnology Co., Ltd., DSM (China) Co., Ltd., Changji Hui Autonomous Prefecture Inspection and Testing Center, Hebei Chenguang Testing Technology Service Co., Ltd., Xinjiang Xinqie Food Co., Ltd., Perfect (Guangdong) Daily Necessities Co., Ltd. Company, Guangdong Health Products Chamber of Commerce. The main drafters of this document are. Zhong Qiding, Liang Jingjing, Wang Daobing, Lian Yunhe, Chen Nannan, Zhou Yajie, Huang Zhaorong, Xu Weiyi, Liang Nan, Wang Xiaoyan, Ma Xiaochun, Yang Qingshan, Yu Chunshan, Li Xiaomin, Jiao Changya, Wu Zhuying, Xu Xiaoying, Liu Ming, Guo Xinguang, Chen Bilian, Yuan Rui, Qian Yifan, Xie Rongxiong, Li Qing, Chen Jingshan, Mao Xuedan, Jiao Liwei, Xiong Suyu, Yin Guangling, Yan Jiangang, Huang Minxin, Ma Pengfei, Hong Meiling. The previous versions of this document and the documents it replaces are as follows. ---First published in.2008 as GB/T 22249-2008; ---This is the first revision. Determination of Lycopene in Health Food1 ScopeThis document describes a method for the determination of lycopene in health foods by HPLC. This document is applicable to the determination of lycopene in health foods in dosage forms such as soft capsules, hard capsules, tablets, and powders.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories3 Terms and definitionsThere are no terms or definitions that require definition in this document.4 PrincipleThe lycopene in the non-microencapsulated sample was extracted or dissolved by an organic solvent to obtain a sample solution; the microencapsulated sample was treated with an ammonia-phosphate buffer solution After the cell wall was broken, the lycopene in the sample was dispersed into the solution, and tetrahydrofuran was added to dissolve it to obtain the sample solution. The products were separated by HPLC and detected by UV detector. The products were qualitatively identified by retention time and quantitatively identified by external standard method.5 Reagents or materialsUnless otherwise specified, only analytical grade reagents were used. 5.1 Reagents 5.1.1 Water. Grade 1 water as specified in GB/T 6682. 5.1.2 Ethanol (C2H5OH). 5.1.3 Petroleum ether. boiling range 60℃~90℃. 5.1.4 N,N-Dimethylformamide (C3H7NO). chromatographically pure. 5.1.5 Preparation of ammonia-phosphate buffer solution. Measure 143 mL of ammonia water, add it to 857 mL of water, mix well, and adjust the pH to 5.5 with phosphoric acid. 9.8±0.1. 5.1.6 Preparation of 0.025% 2,6-di-tert-butyl-p-cresol (BHT) tetrahydrofuran solution. Weigh 0.25 g (accurate to 0.1 mg) of BHT and Add tetrahydrofuran to 1 L and mix well. 5.1.7 Preparation of 0.5% BHT dichloromethane solution. Weigh 2.5 g (accurate to 0.1 mg) BHT into a brown glass container and After ultrasonic dissolution with 100 mL of dichloromethane (chromatographic grade), cool to room temperature, transfer to a 500 mL brown volumetric flask, and Transfer to a brown reagent bottle and store in a sealed container at room temperature away from light. 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