GB/T 21675-2022 English PDF

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GB/T 21675-2022: Diagnostic techniques for African horse sickness
Status: Valid

GB/T 21675: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21675-2022	359	Add to Cart	4 days	Diagnostic techniques for African horse sickness	Valid
GB/T 21675-2008	519	Add to Cart	3 days	Diagnostic techniques for African horse sickness	Obsolete

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 18936   GB/T 21674   

Basic data

Standard ID: GB/T 21675-2022 (GB/T21675-2022)
Description (Translated English): Diagnostic techniques for African horse sickness
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 18,192
Date of Issue: 2022-12-30
Date of Implementation: 2023-07-01
Older Standard (superseded by this standard): GB/T 21675-2008
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 21675-2022: Diagnostic techniques for African horse sickness

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220 CCSB41 National Standards of People's Republic of China Replacing GB/T 21675-2008 African Horse Sickness Diagnostic Technology Posted on 2022-12-30 2023-07-01 implementation State Administration for Market Regulation Released by the National Standardization Management Committee

table of contents

Preface I Introduction II 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Biosecurity measures 2 6 Clinical Diagnosis 2 6.1 Susceptible animals 2 6.2 Clinical symptoms 2 6.3 Pathological changes 3 6.4 Clinical Judgment 3 7 Sample collection and processing 3 7.1 Sample collection 3 7.2 Sample handling 3 8 Laboratory diagnosis 3 8.1 Pathogen isolation and identification 3 8.2 RT-PCR method 4 8.3 General Real-timeRT-PCR method 6 8.4 Type-specific Real-timeRT-PCR method 7 8.5 Indirect ELISA antibody detection method 8 8.6 Blocking ELISA antibody detection method 9 9 comprehensive judgment 10 Appendix A (Normative) Preparation of related solutions for virus isolation 11 Appendix B (Informative) Sequence Information of Primers and Probes for Nucleic Acid Detection 12 Appendix C (Normative) Preparation of related solutions for nucleic acid detection 14 Appendix D (Normative) Preparation of related solutions for enzyme-linked immunosorbent assay 15

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. This document replaces GB/T 21675-2008 "African Horse Sickness Diagnostic Technique". Compared with GB/T 21675-2008, except for structural adjustment and Apart from editorial changes, the main technical changes are as follows. --- Added the chapter "Abbreviations" (see Chapter 4); --- Added "biosecurity measures" (see Chapter 5); --- Added "General Real-timeRT-PCR method" (see 8.3); --- Added "type-specific Real-timeRT-PCR method" (see 8.4); --- Added "blocking ELISA antibody detection method" (see 8.6); --- Deleted "separation of suckling mice" (see 4.1.4 of the.2008 edition); --- Deleted "micro-complement fixation reaction" (see 4.2.2 of the.2008 edition). Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This document was drafted by. China Animal Health and Epidemiology Center, Animal Disease Prevention and Control Center of Inner Mongolia Autonomous Region. The main drafters of this document. Wu Xiaodong, Li Lin, Xu Tiangang, Hu Yongxin, Zou Yanli, Bao Jingyue, Li Jinming, Ren Weijie, Zhao Yonggang, Wang Shujuan, Liu Shan, Gong Mingxia, Zuo Yuanyuan, Yu Xiaojing, Ma Lifeng, Wang Jianlong, Wang Zhiliang. The release status of previous versions of this document and the documents it replaces are as follows. ---It was first published as GB/T 21675-2008 in.2008; --- This is the first revision.

Introduction

A non-contact viral infectious disease caused by equine animals, characterized by changes in the respiratory system and circulatory system, often causing horses, mules At least two species of Culicoides can spread the disease. AHSV belongs to the genus Orbivirus of the family Reoviridae. The disease is currently known to have 9 serotypes type. The World Organization for Animal Health (WOAH) lists it as a notifiable animal disease. my country defines it as a first-class animal disease. The occurrence of this disease has a certain seasonality and regionality, and it is more common in hot and humid seasons. Once it is introduced, it is often endemic or outbreaks. broadcast quickly. Thick frost, high dry terrain, natural barriers and other climatic and geographical conditions that affect the reproduction or activities of vector insects can significantly reduce the disease. The content of diagnostic techniques in this document includes clinical diagnosis, etiological diagnosis methods and serological diagnosis methods. The revision of this document refers to the WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019), and incorporates relevant Chinese technologies. new research results. African Horse Sickness Diagnostic Technology

1 Scope

This document specifies the clinical diagnosis of African horse sickness, sample collection and processing, as well as virus isolation and identification, RT-PCR, general-purpose Real-timeRT-PCR, type-specific Real-timeRT-PCR, indirect ELISA antibody detection, blocking ELISA antibody detection and other experiments Technical requirements for laboratory diagnostic methods. This document is applicable to the diagnosis, detection, quarantine, surveillance and epidemiological investigation of AHS in horses, mules, donkeys and other equines.

2 Normative references

The content in the following documents constitutes the essential provisions of this document through normative references in the text, among which, the dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB 19489 General Requirements for Laboratory Biosafety

3 Terms and Definitions

This document does not have terms and definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document. ABTS. 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (2,2'-azino-di-[3-ethyl-benzothiazoline]-6- sulphonic acid) CPE. cytopathic effect (cytopathic effect) Ct. cycle threshold (cyclethreshold) Cy5.cyanine fluorescent dye (cyanine5) FAM. 6-carboxyfluorescein (6-carboxy-fluorescein) MGB. DNA minor groove binder (minorgroovebinder) ......

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