GB/T 21036-2007 English PDF

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GB/T 21036-2007: Determination of dopamine hydrochloride in feeds -- High performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21036-2007	159	Add to Cart	3 days	Determination of dopamine hydrochloride in feeds -- High performance liquid chromatography	Valid

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Basic data

Standard ID: GB/T 21036-2007 (GB/T21036-2007)
Description (Translated English): Determination of dopamine hydrochloride in feeds -- High performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 6,682
Date of Issue: 2007-06-21
Date of Implementation: 2007-09-01
Regulation (derived from): China National Standard Announcement 2007 No.7 (Total No.107) - National-Standard-Commission
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the determination of feed Dopamine Hydrochloride by HPLC. This standard applies to feed, concentrated feed and determination of dopamine hydrochloride content of any feed premix. The detection limit of this method was 0. 5 mg/kg, the lowest limit of quantification was 1. 7 mg/kg.

GB/T 21036-2007: Determination of dopamine hydrochloride in feeds -- High performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of dopamine hydrochloride in feeds. High performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Determination of dopamine hydrochloride in feed High Performance Liquid Chromatography Posted 2007-06-21 2007-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is the basis of consulting a large number of domestic and foreign literature, depending on the level of technological development of research developed, using high-performance liquid Dopamine hydrochloride content in feed chromatography. The standard proposed by the Ministry of Agriculture, Animal Husbandry Secretary. This standard by the National Standardization Technical Committee of the feed industry. This standard is drafted by. China Agricultural University, the Ministry of Agriculture veterinary drug safety Supervision and Testing Center (Beijing). The main drafters of this standard. ZHANG Su-xia, Shen Jianzhong, Dingshuang Yang, Chenglin Li Jiang ocean. Determination of dopamine hydrochloride in feed High Performance Liquid Chromatography

1 Scope

This standard specifies the determination of dopamine hydrochloride content in feed by HPLC. This standard applies to feed, concentrated feed and feed Determination of Dopamine Hydrochloride premix. The detection limit of the method Of 0.5mg/kg, limit of quantification was 1.7mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples

3 PRINCIPLE OF THE METHOD

With hydrochloric acid solution/methanol extract feed dopamine hydrochloride, after centrifugation to remove solids and some impurities, acidic alumina solid phase Extraction (SPE), after the eluent through membrane filtration HPLC fluorescence detector.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade method, water is deionized water, in line with GB/T 6682 two water regulations. 4.1 Methanol. chromatographically pure. 4.2 ethyl acetate. chromatography. 4.3 sodium sulfide solution. a concentration of 325mg/L, weighed 0.1g sodium sulfide (Na2S · 9H2O) dissolved in water, and dilute to 100mL. 4.4 hydrochloric acid solution. a concentration of 0.1mol/L, take 8.6mL of concentrated hydrochloric acid in 1L flask with water to volume, and mix. 4.5 extract. Take 50mL hydrochloric acid solution (4.4) and 450mL of methanol and mix. 4.6 SPE activation solution. Weigh 4.5g of sodium chloride dissolved in 90mL water, then add 10mL of methanol and mix. 4.7 eluent. Take 450mL of hydrochloric acid solution (4.4) and 50mL of methanol and mix. 4.8 acidic methanol solution. Take 250μL of formic acid dissolved in 500mL of methanol and mix. 4.9 0.02% formic acid solution. take 200μL acid was dissolved in 1000mL of water, and mix. 4.10 dopamine hydrochloride stock standard solution. Weigh standard dopamine hydrochloride (content greater than 98%) 0.1000g in 100mL capacity Bottle with acidic methanol solution (4.8) was dissolved constant volume, formulated at a concentration of 1mg/mL stock solution of dopamine hydrochloride. 4 ℃ under the conditions of storage, Valid for three months. 4.11 dopamine hydrochloride intermediate standard solution. Pipette 10mL stock standard solution (4.10) into 100mL volumetric flask, dilute with acidic methanol And set the volume, formulated at a concentration of 100μg/mL dopamine hydrochloride of intermediate standard solution. At 4 ℃ storage, valid for one month. 4.12 dopamine hydrochloride working standard solution. Pipette dopamine hydrochloride were intermediate standard solution (4.11) 0.025mL, 0.050mL, 0.125mL, 0.250mL, 1.25mL and 2.50mL to 25mL volumetric flask, dilute to volume with water formulated to 0.1μg/mL, 0.2μg/mL, 0.5μg/mL, 1.0μg/mL, 5.0μg/mL and 10.0μg/mL working standard solution. ......

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