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Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 19200-2025: Diagnostic techniques for swine vesicular disease Status: Valid GB/T 19200: Historical versions
Basic dataStandard ID: GB/T 19200-2025 (GB/T19200-2025)Description (Translated English): Diagnostic techniques for swine vesicular disease Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 22,280 Date of Issue: 2025-08-29 Date of Implementation: 2026-03-01 Older Standard (superseded by this standard): GB/T 19200-2003, GB/T 22917-2008 Issuing agency(ies): State Administration for Market Regulation, National Standardization Administration GB/T 19200-2025: Diagnostic techniques for swine vesicular disease---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT19200-2025ICS 11.220 CCSB41 National Standard of the People's Republic of China Replaces GB/T 19200-2003, GB/T 22917-2008 Diagnostic technology of swine vesicular disease Released on August 29, 2025 Implementation on March 1, 2026 State Administration for Market Regulation The National Standardization Administration issued Table of ContentsPreface III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Clinical Diagnosis 2 6 Biosafety Measures 2 7 Sample Collection and Processing 2 8 Virus Isolation 3 9 Reverse transcription-polymerase chain reaction (RT-PCR) 5 10 Fluorescent reverse transcription polymerase chain reaction (fluorescent RT-PCR) 6 11 Virus Neutralization Test (VNT) 8 12 Monoclonal antibody competition enzyme-linked immunosorbent assay (monoclonal antibody competition ELISA) 10 13 Comprehensive judgment 11 Appendix A (Normative) Reagents and Solution Preparation Methods 12 Appendix B (Informative) Preparation of positive control plasmid for RT-PCR detection and test results (see Figure 13) Appendix C (Informative) Preparation of positive control plasmid for fluorescent RT-PCR detection and detection results refer to Figure 14 Appendix D (Informative) Preparation of Standard Positive Serum and Standard Negative Serum 15 Appendix E (Normative) Karber method calculation method 16 Appendix F (Normative) Enzyme-linked immunosorbent assay solution preparation method 17 Preface This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 19200-2003 “Diagnostic Techniques for Swine Vesicular Disease” and GB/T 22917-2008 “Fluorescence Spectroscopy of Swine Vesicular Disease Virus”. RT-PCR detection method". This document is based on GB/T 19200-2003 and integrates the contents of GB/T 22917-2008. Compared with GB/T 19200-2003, in addition to structural adjustments and editorial changes, the main technical changes are as follows. --- Changed the scope (see Chapter 1, Chapter 1 of the.2003 edition); --- Added abbreviations (see Chapter 4); --- Increased clinical diagnosis (see Chapter 5); --- Added biosafety measures (see Chapter 6); --- Added reverse transcription-polymerase chain reaction (RT-PCR) (see Chapter 9); --- Added fluorescent reverse transcription polymerase chain reaction (fluorescent RT-PCR) (see Chapter 10); --- Added monoclonal antibody competition enzyme-linked immunosorbent assay (monoclonal antibody competition ELISA) (see Chapter 12); --- Added comprehensive judgment (see Chapter 13); --- Deleted the reverse indirect hemagglutination test (RIHA) (see 2.5 of the.2003 edition); --- Deleted agar gel immunodiffusion test (AGID) (see Chapter 3 of the.2003 edition). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181). This document was drafted by. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, and Shenzhen Customs Animal and Plant Inspection and Quarantine Technical Center. The main drafters of this document are. Zheng Haixue, Yang Fan, He Jijun, Ma Weimin, Shen Chaochao, Cao Weijun, Ruan Zhouxi, Zhu Zixiang, Yang Yamin, Lü Lu, Liu Yongjie, Lin Yanxing, Hua Qunyi, Guo Jianhong, and Liu Xiangtao. The previous versions of this document and the documents it replaces are as follows. ---First published in.2003 as GB/T 19200-2003; --- This is the first revision, incorporating the technical content of GB/T 22917-2008.introductionAn acute, contagious disease of pigs characterized by blisters around the hooves, mouth, snout, and teats, with no obvious signs of infection in the internal organs. SVDV belongs to the Picornaviridae family, Enterovirus genus, and has only one The World Organization for Animal Health (WOAH) lists it as a notifiable disease, and my country defines it as a Class I animal disease. Diagnostic technology of swine vesicular disease1 ScopeThis document describes the clinical diagnosis of swine vesicular disease, laboratory diagnostic sample collection and processing, as well as virus isolation, reverse transcription-polymerase chain reaction Laboratory diagnostic methods include PCR, fluorescent reverse transcription-polymerase chain reaction, virus neutralization test, monoclonal antibody competitive enzyme-linked immunosorbent assay, etc. This document applies to the diagnosis, quarantine, detection, monitoring and epidemiological investigation of swine vesicular disease.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB 19489 General requirements for laboratory biosafety3 Terms and DefinitionsThere are no terms or definitions that require definition in this document.4 AbbreviationsThe following abbreviations apply to this document. BEI. Binaryethylenimine OD. Optical density RNA. Ribonucleic acid ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 19200-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 19200-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 19200-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 19200-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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