GB/T 18654.13-2008 English PDF

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GB/T 18654.13-2008: Inspection of germplasm for cultured fishes -- Part 13: Analysis of isozyme electrophoresis
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GB/T 18654.13-2008	189	Add to Cart	3 days	Inspection of germplasm for cultured fishes -- Part 13: Analysis of isozyme electrophoresis	Valid

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SC/T 4030   GB 5009.261   GB/T 18654.12   GB/T 18654.14   GB/T 18654.11   

Basic data

Standard ID: GB/T 18654.13-2008 (GB/T18654.13-2008)
Description (Translated English): Inspection of germplasm for cultured fishes -- Part 13: Analysis of isozyme electrophoresis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B50
Classification of International Standard: 65.150
Word Count Estimation: 8,816
Date of Issue: 2008-06-27
Date of Implementation: 2008-10-01
Quoted Standard: GB/T 18654.1-2002; GB/T 18654.2
Regulation (derived from): National Standard Approval Announcement 2008 No.11 (Total No.124)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the reagents and materials fish isozymes polyacrylamide gel electrophoresis analysis of the level, instruments and equipment, sampling, analysis procedures and results of the determination. This section applies to fish in isoenzyme electrophoresis analysis.

GB/T 18654.13-2008: Inspection of germplasm for cultured fishes -- Part 13: Analysis of isozyme electrophoresis

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Inspection of germplasm for cultured fishes Part 13. Analysis of isozyme electrophoresis ICS 65.150 B50 National Standards of People's Republic of China Inspection of germplasm for cultured fishes Part 13. isoenzyme electrophoresis analysis Posted 2008-06-27 2008-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 18654 "Inspection of germplasm for cultured fishes" is divided into the following sections. --- Part 1. Inspection rules; --- Part 2. Sampling methods; --- Part 3. Determination of traits; --- Part 4. Determination of age and growth; --- Part 5. Diet analysis; --- Part 6. Determination of reproductive performance; --- Part 7. Ecological Characteristics; --- Part 8. Determination of the oxygen consumption rate and the critical point of suffocation; --- Part 9. Includes meat for determination; --- Part 10. Determination of muscle nutrients; --- Part 11. Determination of the major muscle amino acid contents; --- Part 12. karyotype analysis; --- Part 13. isoenzyme electrophoresis analysis; --- Part 14. Determination of DNA content; --- Part 15. RAPD analysis; This section GB/T Part of 1,318,654. This section of Appendix A normative appendix. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee of aquatic freshwater aquaculture division Technical Committee. This section is drafted. Shanghai Fisheries University, Chinese Academy of Fishery Sciences, Yangtze River Fisheries Research Institute. The main drafters of this section. Li Sifa, Zhao Jinliang, Xu Zhong law, CAI Wan, Zou Shuming. Inspection of germplasm for cultured fishes Part 13. isoenzyme electrophoresis analysis

1 Scope

This section GB/T 18654 specifies the level of fish isozymes polyacrylamide gel electrophoresis analysis of the reagents and materials, equipment and set up Equipment, sampling, analysis procedures and the results of determination. This section applies to fish isoenzyme electrophoresis analysis.

2 Normative references

The following documents contain provisions which, through reference GB/T 18654 in this section constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB/T 18654.1-2002 test germplasm for cultured fishes - Part 1. Inspection Rules GB/T 18654.2 Inspection of germplasm for cultured fishes - Part 2. Sampling Method Principle 3 The isozyme phenotypes enzyme gene product, depending on the charge brought and different molecular size, shape, and an electric field in the gel Each component appearing in different mobilities isozyme, catalytic, staining, scanning, according to the enzyme with the migration distance, the number and intensity of the absorption analysis , And it determines the genetic characteristics of fish species populations.

4 Reagents and materials

Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 4.1 acrylamide (Arc). 4.2 N ', N'- methylene bisacrylamide or methylene-bis-acrylamide (Bis). 4.3 tetramethylethylenediamine (TEMED). 4.4 ammonium persulfate. 4.5 Tris (Tris). 4.6 citric acid. 4.7 ethylenediaminetetraacetic acid (EDTA). 4.8 boric acid. 4.9 L- histidine. 4.10 lactic acid. 4.11 DL- malic acid. 4.12 α- glycerophosphate sodium. 4.13 disodium hydrogen phosphate (Na2HPO4). 4.14 two crystal water of sodium dihydrogen phosphate (NaH2PO4 · 2H2O). 4.15 coenzyme Ⅰ (NAD). 4.16 coenzyme Ⅱ (NADP). ......

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