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GB/T 18448.2-2008 English PDF

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US$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 18448.2-2008: Laboratory animal -- Method for examination of Toxoplasma gondii
Status: Valid

GB/T 18448.2: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 18448.2-2008	229	Add to Cart	3 days	Laboratory animal -- Method for examination of Toxoplasma gondii	Valid
GB/T 18448.2-2001	199	Add to Cart	2 days	Laboratory animal -- Methods for examination of Toxoplasma gondii	Obsolete

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Basic data

Standard ID: GB/T 18448.2-2008 (GB/T18448.2-2008)
Description (Translated English): Laboratory animal -- Method for examination of Toxoplasma gondii
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B44
Classification of International Standard: 65.020.30
Word Count Estimation: 10,167
Date of Issue: 2008-12-03
Date of Implementation: 2009-03-01
Older Standard (superseded by this standard): GB/T 18448.2-2001
Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the experimental animals Toxoplasma gondii detection methods and reagents. This section applies to mice, rat, hamster, guinea pig, rabbit, dogs and monkeys and other animals to detect Toxoplasma gondii.

GB/T 18448.2-2008: Laboratory animal -- Method for examination of Toxoplasma gondii

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Method for examination of Toxoplasma gondii ICS 65.020.30 B44 National Standards of People's Republic of China Replacing GB/T 18448.2-2001 Experimental animals Toxoplasma gondii detection method Posted 2008-12-03 2009-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 18448 "animal" by the 10 animal parasite detection components. This section GB/T 18448 Part 2 "animals Toxoplasma gondii detection methods." This section instead of the date of implementation of the GB/T 18448.2-2001. This part of the main technical differences compared with the GB/T 18448.2-2001 as follows. a) increase Toxoplasma enzyme linked immunosorbent assay (ELISA) and immunohistochemical staining test (IEA), the PCR detection methods listed in Appendix Content, retaining indirect hemagglutination test (IHA) as one of the recommended methods; b) the required detection method, "Materials and Reagents" and "detection step" described separately. Appendix A of this section is normative appendix. This part of the National Standardization Technical Committee of experimental animals and focal points. This section drafted by. National Standardization Technical Committee of experimental animals. The main drafters of this section. PAN Zhen industry, Quxia Qin, Chen Qiao Mei, Li Guanmin, WANG Yan-ping. This part of the standard replaces the previous editions are. --- GB/T 14926.34-1994, GB/T 18448.2-2001. Experimental animals Toxoplasma gondii detection method

1 Scope

GB/T 18448 provisions of this part of the experimental animals Toxoplasma gondii detection methods and reagents. This section applies to mice, rats, hamsters, guinea pigs, rabbits, dogs and monkeys and other animals Toxoplasma gondii detection. Principle 2 According to the principles of immunology, Toxoplasma antigen detection using animal serum antibody detection of Toxoplasma gondii.

3 Reagents and equipment

3.1 Reagents 3.1.1 ELISA antigen 3.1.1.1 Specific Antigen Toxoplasma gondii (RH strain) tachyzoites inoculated intraperitoneally cleaning stage above mice (KM, ICR, BALB/c, etc. can be), after the 3d ~ 5d, to Sterile saline lavage of the abdominal cavity of mice vaccinated, mouse peritoneal fluid was collected containing the worm, 3000r/min centrifugal 10min, the precipitate, PBS three times. Add appropriate amount of distilled water to precipitate five times repeated freezing and thawing, or ultrasonic treatment, 10000r/min centrifugal 30min, take on Serum. Supernatant Sephadex G200 gel (column diameter × column height. 1.5cm × 60cm), a flow rate of 0.2mL/min. each Collection tube about 2mL. We collected a total of 60 or more. Each collection tube protein absorbance values \u200b\u200bwere measured at 280nm under. Isolation of pure After technology, the emergence of two protein peaks; the first peak of the tubes were combined, namely Toxoplasma-specific antigen (also known as Toxoplasma gondii soluble antigen). 3.1.1.2 normal antigen Sterile saline injection cleaning stage above mice (mice with an antigen preparation of the same species or strain) intraperitoneal, after 3d ~ 5d, to Sterile saline is injected in murine peritoneal lavage collected mouse peritoneal fluid, 3000r/min centrifugal 10min, the precipitate, PBS washed 3 times. Add appropriate amount of distilled water to precipitate five times repeated freezing and thawing, or ultrasonic treatment, 10000r/min centrifugal 30min, the supernatant is normal antigen. 3.1.2 antigen sheet Toxoplasma gondii RH tachyzoites inoculated intraperitoneally cleaning stage above mice (KM, ICR, BALB/c, etc. can be), 3d ~ 5d at the rear Dead, collect worms, trypsin, to a certain concentration smear, fully dry after cold acetone fixed, -20 ℃ preservation. 3.1.3 Toxoplasma antigen-sensitized sheep red blood cells The tannic acid was reacted with a certain concentration of sheep red blood cells, red blood cells formed tanning, then, with Toxoplasma gondii soluble antigen in a suitable strip Member under tanning sensitized red blood cells, made of Toxoplasma antigen-sensitized sheep red blood cells. 3.1.4 normal sheep red blood cells. 3.1.5 positive control serum Naturally infected with Toxoplasma corresponding animal antibody positive serum or serum Toxoplasma gondii. 3.1.6 negative control serum No confirmed toxoplasmosis animal serum. 3.1.7 conjugate Horseradish peroxidase-labeled anti-mouse, rat, hamster, guinea pigs, rabbits, dogs and monkeys IgG antibody; or horseradish peroxidase-labeled Portugal Aureus protein A (SPA). 3.1.8 fluorescein conjugates Isothiocyanate hydrogen acid fluorescein-labeled anti-mouse, rat, hamster, guinea pigs, rabbits, dogs and monkeys IgG antibodies. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 18448.2-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 18448.2-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 18448.2-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 18448.2-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 18448.2-2008?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 18448.2-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.