GB/T 18420.2-2009 English PDFUS$719.00 · In stock
Delivery: <= 6 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 18420.2-2009: Biological toxicity for pollutants from marine petroleum exploration and exploitation -- Part 2: Test method Status: Valid GB/T 18420.2: Historical versions
Basic dataStandard ID: GB/T 18420.2-2009 (GB/T18420.2-2009)Description (Translated English): Biological toxicity for pollutants from marine petroleum exploration and exploitation -- Part 2: Test method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: Z17 Classification of International Standard: 13.030.20 Word Count Estimation: 31,342 Date of Issue: 2009-03-11 Date of Implementation: 2009-11-01 Older Standard (superseded by this standard): GB/T 18420.2-2001 Quoted Standard: GB 17378.3, GB 17378.4-2007 Regulation (derived from): Announcement of Newly Approved National Standards 2009 No. 2 (total 142) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the offshore oil exploration and development operations and after use or generate some pollutants discharged into the sea in the biological tests for toxicity test methods used, and the test methods for sample handling, experimental biology, test procedures and results determined that quality control requirements. This section applies to offshore oil exploration and development operations and the use or generation after drilling into the sea, the base fluid, drill cuttings and production water toxicity tests, the partially qualified outside contaminants can refer to the use of biological toxicity tests. GB/T 18420.2-2009: Biological toxicity for pollutants from marine petroleum exploration and exploitation -- Part 2: Test method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Biological toxicity for pollutants from marine petroleum exploration and exploitation Part 2. Test method ICS 13.030.20 Z17 National Standards of People's Republic of China Replacing GB/T 18420.2-2001 Offshore oil exploration and exploitation of biological toxicity of pollutants Part 2. Test methods Posted 2009-03-11 2009-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released Table of ContentsPreface Ⅰ 1 Scope 1 2 Normative references 1 3 Terms and definitions 4 Principle 1 5 sampling, sample preservation and sample 2 6 Experimental Biology 2 Facilities and equipment 3 7 8 Test Procedure 4 9 Analysis of test results and determine 6 10 6 Quality Control 11 inspection reports 6 Appendix A (informative) toxicity test results in Table 7 original records Appendix B (normative) toxicity test result determination flowchart 11 Calculation Appendix C (Informative Appendix) LC50 and EC50 of 13 Appendix D (Informative Appendix) Spearman-Karber calculation and TrimmedSpearman-Karber Calculation method 17 Annex E (informative) test of normality and homogeneity of variance test 19 Appendix F (Informative Appendix) Dunnett's test and Steel's many-rank test 22 Annex G (informative) toxicity test report form 26 References 27Foreword"Offshore Oil Exploration and Development pollutant toxicity" is divided into two parts. --- Part 1. Classification; --- Part 2. Test methods. This is Part 2. This Part replaces GB/T 18420.2-2001 "marine pollutant biological toxicity test method of petroleum exploration and development." This section compared with the GB/T 18420.2-2001 The main changes are as follows. --- The terms and definitions of the terms "96h changing the water biological toxicity test" and modify the definition of "hydrostatic biological toxicity test" (2001 version 3.2, this version 3.1), an increase of "EC50" Terms and Definitions (edition 3.4); --- In the "sampling, sample preservation and sample" added details about a "radical solution" of (this version 5.1.1 and 5.1.2.2); --- In the "Experimental Biology" increased four biological experiments and related content (edition 6.1 to 6.4) a chapter; --- To increase the "base fluid" and "synthetic drilling fluid" method of preparation of stock solution (Excerpts 8.1.2.1.3 and 8.1.2.2); --- Increased to grow as observations related to the operation of biological toxicity test methods (this edition 8.4.6.2.2); --- Revised part of "validity of the results" (2001 version 10.1, this version 9.1); --- Increased partial example of a method of data processing and calculation results in the appendix, as well as original records growth toxicity test table (Excerpts Appendix A ~ Appendix F). Appendix B to this part of the normative appendix, Appendix A, Appendix C, Appendix D, Appendix E, Appendix F, Appendix G is an informative annex. This part by the State Oceanic Administration. This part of the National Standardization Technical Commission for Oceanography (SAC/TC283) centralized. This section is drafted. Guangdong Experimental Animals Monitoring Institute of the South China Sea Branch of the State Oceanic Administration. Drafters of this section. Huang Ren Wei community forest, Wujin Xiao, Zheng Yanjing, Wang Ying, Wang Guodong, Zhou Jin. This part of the standard replaces the previous editions are. --- GB/T 18420.2-2001. Offshore oil exploration and exploitation of biological toxicity of pollutants Part 2. Test methods1 ScopeThis section provides offshore oil exploration and exploitation and discharged after use or generate pollutants in the marine part of the biological toxicity test Testing methods, and test method treatment, biological experiments, test procedures for sample results to determine the quality control requirements. This section applies to offshore oil exploration and exploitation in the use or generation and into the sea after drilling base fluids, drill cuttings and produced water Biological toxicity testing, biological toxicity tests in this section defining an outer pollutants can refer to use.2 Normative referencesThe following documents contain provisions which, through reference in this text, constitute provisions of this part. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this section, however, encourage the parties to this part of the research agreement Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB 17378.3 specification for marine monitoring Part 3. Sample collection, storage and transportation GB 17378.4-2007 specification for marine monitoring Part 4. Seawater Analysis3 Terms and DefinitionsThe following terms and definitions apply to this section. 3.1 During the test does not replace the internal test solution was observed to measure the biological effects test abnormalities or death. 3.2 Biological experiments after exposure to different concentrations of a test solution of a predetermined time, the adverse effects observed by the biological experiments and comparative test Group and the control group, statistical analysis, determined that no significant effect on the maximum concentration of Experimental Biology. 3.3 LC50 In certain observation period, resulting in a 50% concentration of toxicant test biological death. [GB 17378.7-2007, the definition 3.10] 3.4 EC50 In certain observation period, causing 50% of the test organisms in a particular effect or an effect index is suppressed a biological experiment Toxicant concentration and a half to EC50 expressed. Principle 4 Various physiological activities of marine organisms by marine environmental impact, measured by observing the biological experiments in a certain period of contact with the test Test substance Abnormal or qualitative effect after death, obtain offshore petroleum exploitation specific pollutant concentrations had a significant effect on the test organisms. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 18420.2-2009_English be delivered?Answer: Upon your order, we will start to translate GB/T 18420.2-2009_English as soon as possible, and keep you informed of the progress. The lead time is typically 4 ~ 6 working days. 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