GB/T 17816-2025 English PDF

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GB/T 17816-2025: Determination of total ascorbic acid in feeds
Status: Valid

GB/T 17816: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17816-2025	219	Add to Cart	3 days	Determination of total ascorbic acid in feeds	Valid
GB/T 17816-1999	159	Add to Cart	2 days	Determination of total ascorbic acid in feeds. o-Phenylenediamine fluorometry

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Basic data

Standard ID: GB/T 17816-2025 (GB/T17816-2025)
Description (Translated English): Determination of total ascorbic acid in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 10,123
Date of Issue: 2025-08-01
Date of Implementation: 2026-02-01
Older Standard (superseded by this standard): GB/T 17816-1999
Issuing agency(ies): State Administration for Market Regulation, Standardization Administration of China

GB/T 17816-2025: Determination of total ascorbic acid in feeds

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT17816-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 17816-1999 Determination of total ascorbic acid in feed Released on August 1, 2025 Implementation on February 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 17816-1999 "Determination of total ascorbic acid in feeds - o-phenylenediamine fluorescence method" and GB/T 17816- Compared with.1999, in addition to structural adjustments and editorial changes, the main technical changes are as follows. --- Changed the detection limit of o-phenylenediamine fluorescence method and added the limit of quantification (see Chapter 1, Chapter 1 of the.1999 edition); --- Added high performance liquid chromatography determination method (see Chapter 4); --- Changed the calculation and presentation of o-phenylenediamine fluorescence analysis results (see 5.5.4, Chapter 8 of the.1999 edition). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Guoliang Wuhan Scientific Research and Design Institute Co., Ltd. The main drafters of this document are. Shao Rui, Du Yan, Chen Xiyu, Wang Boyuan, Chen Mengying, Ma Shenghui, Wang Zhongyan, Yan Hongyue, Su Yongbo, Qiu Huijuan, Chang Xiaohong, Wei Shuangzheng, and Liu Xiaomin. The previous versions of this document and the documents it replaces are as follows. ---First published in.1999 as GB/T 17816-1999; ---This is the first revision. Determination of total ascorbic acid in feed

1 Scope

This document describes a high performance liquid chromatography and o-phenylenediamine fluorometric method for the determination of total ascorbic acid in feeds. This document's high performance liquid chromatography method is applicable to the determination of L-ascorbic acid, D-isoascorbic acid in compound feed, concentrated feed, and additive premix feed. The o-phenylenediamine fluorescence method is applicable to the determination of total ascorbic acid in compound feed, concentrated feed, and additive premix feed. Determination of. This document is not applicable to the determination of total ascorbic acid in feeds added in the form of esterified ascorbic acid. The detection limits of L-ascorbic acid and D-isoascorbic acid by HPLC in this document are both 5 mg/kg, and the quantification limits are both The detection limit of o-phenylenediamine fluorescence method is 8 mg/kg, and the quantification limit is 25 mg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document. 4 High-performance liquid chromatography 4.1 Principle Ascorbic acid in the sample was extracted with metaphosphoric acid solution and determined by high performance liquid chromatography using the external standard method. 4.2 Reagents or materials Unless otherwise specified, only analytical grade reagents were used. 4.2.1 Water. GB/T 6682, Grade 1. 4.2.2 L-cysteine. high purity. 4.2.3 Potassium dihydrogen phosphate. chromatographically pure. 4.2.4 Hexadecyltrimethylammonium bromide. chromatographically pure. 4.2.5 Phosphoric acid. chromatographically pure. 4.2.6 Methanol. chromatographic grade. 4.2.7 Metaphosphoric acid solution (20 g/L). Weigh 20 g of metaphosphoric acid, dissolve it in water and dilute to 1 L, mix well. Store at 4°C. The expiration date is 1 month. 4.2.8 Trisodium phosphate solution (100 g/L). Weigh 100 g of trisodium phosphate, dissolve it in water and dilute to 1 L, then mix thoroughly. ......

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