GB/T 17494-2023 English PDF

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GB/T 17494-2023: Diagnostic techniques for equine infectious anemia
Status: Valid

GB/T 17494: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17494-2023	419	Add to Cart	4 days	Diagnostic techniques for equine infectious anemia	Valid
GB/T 17494-2009	359	Add to Cart	3 days	Diagnostic techniques of indirect ELISA technique for equine infectious anemia disease	Obsolete
GB/T 17494-1998	279	Add to Cart	3 days	Rules of indirect ELISA technique for equine infectious anemia disease	Obsolete

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Basic data

Standard ID: GB/T 17494-2023 (GB/T17494-2023)
Description (Translated English): Diagnostic techniques for equine infectious anemia
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 22,295
Date of Issue: 2023-09-07
Date of Implementation: 2024-04-01
Older Standard (superseded by this standard): GB/T 17494-2009
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 17494-2023: Diagnostic techniques for equine infectious anemia

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220 CCSB41 National Standards of People's Republic of China Replace GB/T 17494-2009 Diagnostic Technology for Equine Infectious Anemia Published on 2023-09-07 2024-04-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration Committee

Table of contents

Preface III Introduction IV 1 Scope 1 2 Normative reference documents 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Laboratory biosafety measures 2 6 Clinical Diagnosis 2 7 Sample collection, storage and transportation3 8 Real-time PCR 4 9 Virus isolation and identification5 10 Agar gel immunodiffusion test 6 11 Indirect ELISA 8 12 Competitive ELISA 10 13 Western blot assay 11 14 Comprehensive judgment 13 Appendix A (normative) Preparation of reagents 14 Appendix B (informative) Real-time fluorescence PCR primers and probes 16 Appendix C (informative) Preparation of real-time fluorescence PCR positive control plasmid 17

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. This document replaces GB/T 17494-2009 "Indirect ELISA Diagnostic Technology for Equine Infectious Anemia" and is the same as GB/T 17494-2009 In comparison, in addition to structural adjustments and editorial changes, the main technical changes are as follows. --- Added clinical diagnosis of equine infectious anemia (see Chapter 6); --- Added real-time fluorescence PCR detection of equine infectious anemia virus (see Chapter 8); ---Added the isolation and identification of equine infectious anemia virus (see Chapter 9); ---Added equine infectious anemia agar gel immunodiffusion test (see Chapter 10); ---Changed the indirect ELISA for equine infectious anemia (see Chapter 11, Chapter 4 and 5 of the.2009 edition); ---Added equine infectious anemia competition ELISA (see Chapter 12); ---Added equine infectious anemia immunoblotting test (see Chapter 13); Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents. This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This document was drafted by. Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Xinjiang Uygur Autonomous Region Animal Disease Prevention and Control Center, Guangzhou Customs of the People's Republic of China, China Agricultural University, Beijing Customs of the People's Republic of China. The main drafters of this document. Hu Zhe, Wang Xiaojun, Guo Wei, Lin Yuezhi, Wang Xuefeng, Wang Wen, Chen Ru, Wang Qin, and Bai Yaduo. The previous versions of this document and the documents it replaces are as follows. ---First published as GB/T 17494-2009 in.2009; ---This is the first revision.

Introduction

Category II animal diseases stipulated in the "List of Epidemic Diseases" (2022). EIAV only infects animals of the genus Equus, of which horses are the most susceptible. Infected animals are the main source of infection. After EIAV infects the host, the virus will The body's genome is integrated into the host cell genome, causing persistent host infection and lifelong virus transmission. Clinically, sick animals may show repeated fever, Anemia, jaundice, hemorrhage, edema and emaciation are the characteristics of the disease. However, a certain proportion of horses and most donkeys and mules do not show obvious clinical symptoms after infection. As latent virus carriers. There is currently no available vaccine for this disease. The diagnosis of this disease requires laboratory diagnosis. EIAV belongs to reverse transcription After entering the host cell, the virus and EIAV replicate in the early stage under the action of viral reverse transcriptase to form double-stranded viral DNA (often called provirus). DNA), the proviral DNA is further integrated into the host chromosome under the action of the virus-encoded integrase. EIAV proviral DNA It is relatively stable in the infected host and is often used as a target for viral nucleic acid detection. There are relatively few EIAV genome sequences in different regions of the world. There is a big difference. The nucleic acid detection method used in this document is a universal real-time fluorescence PCR method suitable for different current strains. Diagnostic Technology for Equine Infectious Anemia

1 Scope

This document describes the clinical diagnosis of EIA, real-time fluorescence PCR, virus isolation and identification, agar gel immunodiffusion test, indirect Methods of ELISA, competitive ELISA, and western blot assays. This document applies to the diagnosis, quarantine, surveillance and epidemiological investigation of EIA.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative citations in the text. Among them, dated references For referenced documents without dates, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document. document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB 19489 General requirements for laboratory biosafety NY/T 541 Technical specifications for collection, storage and transportation of veterinary diagnostic samples

3 Terms and definitions

There are no terms or definitions to be defined in this document.

4 Abbreviations

The following abbreviations apply to this document. BCA. Bicinchoninic Acid OD value. optical density value (OpticalDensity) Electrophoresis) ......

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