GB/T 16285-2008 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 16285-2008: Determination of glucose in food -- Enzyme -- Colorimetric method and enzyme -- Electrode method Status: Valid GB/T 16285: Historical versions
Basic dataStandard ID: GB/T 16285-2008 (GB/T16285-2008)Description (Translated English): Determination of glucose in food -- Enzyme -- Colorimetric method and enzyme -- Electrode method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.050 Word Count Estimation: 10,145 Date of Issue: 2008-06-25 Date of Implementation: 2009-01-01 Older Standard (superseded by this standard): GB/T 16285-1996 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Approval Announcement 2008 No.10 (Total No.123) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the enzyme-colorimetric and enzyme-Determination of glucose electrode method of analysis steps. This standard applies to the determination of glucose in various types of food, also applies to other components of food into the determination of glucose. This standard enzyme-lowest detection limit of colorimetric 0. 01��g/mL, enzyme-the lowest limit of detection electrode method 1. 0mg/100mL. GB/T 16285-2008: Determination of glucose in food -- Enzyme -- Colorimetric method and enzyme -- Electrode method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detrmination of glucose in food. Enzyme. Colorimetric method and enzyme. Electrode method ICS 67.050 X04 National Standards of People's Republic of China Replacing GB/T 16285-1996 Determination of glucose Enzyme - colorimetric method and enzyme - electrode Posted 2008-06-25 2009-01-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard replaces GB/T 16285-1996 "Determination of food enzyme glucose - colorimetric method and enzyme - electrode method." This standard and GB/T 16285-1996 compared to the main changes are as follows. --- Standard name changed to. Determination of glucose in food enzyme - colorimetric method and enzyme - electrode method; --- According to the provisions of GB/T 1.1-2000 and GB/T 20001.4-2001 and modify the text format. The standard enzyme - colorimetric method of arbitration; Enzymes - Electrode for quick method. This standard Annex A, Annex B normative appendix. This standard by the National Standardization Technical Committee of Food Industry and focal points. This standard was drafted. China State Farms Northern Foods Monitoring Center (Enzyme - colorimetric method), Institute of Biology, Shandong Academy of Sciences (enzyme - electric Pole method). The main drafters. Zhang City, Liu Ning, Feng Derong, Sun Shiqing, Song Jiahua. This standard replaces the standards previously issued as follows. --- GB/T 16285-1996. Determination of glucose Enzyme - colorimetric method and enzyme - electrode1 ScopeThis standard specifies the enzyme - colorimetric method and enzyme - Determination of glucose electrode analysis steps. This standard applies to the determination of various types of food glucose; also applies to the other components of the food is converted to glucose measurement. The standard enzyme - colorimetric minimum detectable limit of 0.01μg/mL; Enzyme - The minimum detection limit of the electrode 1.0mg/100mL.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 Water Analysis Laboratory specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987) 3 enzyme - colorimetric 3.1 Method summary Glucose oxidase (the GOD) under aerobic conditions, the catalytic β-D- glucose (aqueous solution of glucose) oxidation reaction of D- glucose -δ- Acid lactone and hydrogen peroxide. By peroxidase (POD) catalyzed hydrogen peroxide with 4-aminoantipyrine to form a red quinone Abigail forest and phenol amine. Quinone imine measured absorbance at 505nm wavelength, calculate the content of glucose in food. 3.2 Scheme C6H12O6 + O2 → GOD C6H10O6 + H2O2 H2O2 + C6H5OH + C11H13N3O → POD C6H5NO + H2O 3.3 Reagents and solutions 3.3.1 Reagents and analysis of water All reagents were analytical reagent use, or biochemical reagents; analysis of water should comply with two water specifications GB/T 6682 regulations, or redistilled Distilled water. 3.3.2 Combo Kits 1 Bottle. contains phosphate buffer solution (0.2mol/L, pH = 7.0) 100mL, wherein 4-amino-antipyrine was 0.00154mol/L. 2 bottles. containing phenol solution (0.022mol/L) 100mL. 3 bottles. containing glucose oxidase (glucoseoxidase) 400U (activity units), peroxidase (horseradish, peroxidase) 1000U (activity units). Vitality glucose oxidase and peroxidase requirements and determination Appendix A. 1,2,3 bottle should be kept at about 4 ℃. 3.3.3 enzyme reagent solution Will be on the 1st bottle (3.3.2) and 2 bottles (3.3.2) fully mix the contents evenly, then No. 3 bottles (3.3.2) dissolved its contents Gently shake (do not shake it vigorously), glucose oxidase and peroxidase completely dissolved. This solution should be at about 4 ℃ preservation, valid 1 month. Weigh 3.7g of potassium ferrocyanide, dissolved in 100mL of water, shake well. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 16285-2008_English be delivered?Answer: Upon your order, we will start to translate GB/T 16285-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 16285-2008_English with my colleagues?Answer: Yes. 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