GB/T 14927.1-2008 English PDFUS$599.00 · In stock
Delivery: <= 5 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 14927.1-2008: Laboratory animal -- Methods for biochemical markers of inbred mice and rats Status: Valid GB/T 14927.1: Historical versions
Basic dataStandard ID: GB/T 14927.1-2008 (GB/T14927.1-2008)Description (Translated English): Laboratory animal -- Methods for biochemical markers of inbred mice and rats Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B44 Classification of International Standard: 65.020.30 Word Count Estimation: 26,235 Date of Issue: 2008-12-10 Date of Implementation: 2009-03-01 Older Standard (superseded by this standard): GB/T 14927.1-2001 Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard provides for the inbred mouse, biochemical markers were detected in rat cellulose acetate membrane (board, . Dural " electrophoretic methods and criteria in this section apply to inbred strains of mice and rats, no. GB/T 14927.1-2008: Laboratory animal -- Methods for biochemical markers of inbred mice and rats---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Laboratory animal.Methods for biochemical markers of inbred mice and rats ICS 65.020.30 B44 National Standards of People's Republic of China Replacing GB/T 14927.1-2001 Animal inbred mice, Biochemical marker assay in rats Posted 2008-12-10 2009-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordGB/T 14927 is divided into two parts. --- Part 1 as "experimental animals inbred mice, rats and biochemical assay mark"; --- Part 2 as "experimental animals inbred mice, rats immunolabeling assay." This section GB/T Part of 114,927. Since the implementation of this section instead of GB/T 14927.1-2001 "animal inbred mice, rats and biochemical markers detected method". This part of the main technical differences compared with the GB/T 14927.1-2001 as follows. a) schematic diagram based on the results of electrophoresis mouse, rat biochemical marker detection method inbred lines on the increase electrophoresis pattern; b) Adjustment of inbred mice, rats and biochemical markers of biochemical detection method of loci, mice increased peptidase -3 (pep3) bits Point, rat Added serum alkaline phosphatase (Alp) and hemoglobin (Hbb) two biochemical sites; c) on the part of solution formulation adjustments. This section of the Appendix A, Appendix B, Appendix C and Appendix D is an informative annex. This part of the National Standardization Technical Committee of experimental animals and focal points. This section drafted by. National Standardization Technical Committee of experimental animals. The main drafters of this section. Xing Ruichang, Liu bicyclic, Yuebing Fei, Bao Shimin, Zhang Lianfeng. This part of the standard replaces the previous editions are. --- GB/T 14927.1-1994, GB/T 14927.1-2001. Animal inbred mice, Biochemical marker assay in rats1 ScopeThis section GB/T 14927 specifies the inbred mice, rats detect biochemical markers of cellulose acetate membrane (plate, dura mater) electric Swimming methods and criteria. This section applies to inbred strains of mice and rats no.2 Terms and definitionsThe following terms and definitions apply GB/T 14927 in this section. 2.1 It showed that the genetic characteristics and the use of biochemical methods to identify the mark. In a small, rat for a number of isoenzymes and heterogeneous proteins. 2.2 Various inbred summarizing multiple genetic markers and biochemical phenotype data, reflecting the genetic features of the various strains to some extent. 2.3 Having the same status relative positions of genes on homologous chromosomes. Inbred animal mating most of the sites through successive kin They have homozygosity. Within a line between any progeny produced by mating individuals also homozygous. 2.4 A inbred strains all individuals genetically homologous. Therefore, in the same line of skin between individuals and any tumor transplantation Not be regarded as alien and exclusion. Such as inbred animal genes to detect genotypes of different individuals within a line exactly. 2.5 Whole inbred animals, each line is genetically unique performance in a wide range of features, such as genetic and biochemical Overview Appearance and the like. Most inbred strains can be distinguished from each other through their respective biochemical genetic profile.3 PRINCIPLE OF THE METHODIn mice and rats, there are some isozymes and isoforms with proteins. It can be carried in accordance with their specific electric charge different field Electrophoretic method to distinguish them, and that is a protein genotype phenotype infer its type according to electrophoresis, to establish genetic variety inbred Profile, quality monitoring them regularly.4 Equipment and Materials4.1 Atmospheric electrophoresis (0 ~ 600V). 4.2 cellulose acetate membrane (plate, dura mater) electrophoresis tank. 4.3 Electrophoresis spotting devices. 4.4 cellulose acetate membrane (7cm × 9cm). ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 14927.1-2008_English be delivered?Answer: Upon your order, we will start to translate GB/T 14927.1-2008_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version GB/T 14927.1-2008?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 14927.1-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
