GB/T 14926.57-2008 English PDF

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GB/T 14926.57-2008: Laboratory animal -- Method for examination of canine parvovirus
Status: Valid

GB/T 14926.57: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 14926.57-2008	149	Add to Cart	2 days	Laboratory animal -- Method for examination of canine parvovirus	Valid
GB/T 14926.57-2001	199	Add to Cart	2 days	Laboratory animal -- Method for examination of Canine parvovirus (CPV)	Obsolete

Similar standards

GB/T 43051   GB/T 39760   GB/T 35823   GB/T 14926.46   GB/T 14926.47   GB/T 14926.58   

Basic data

Standard ID: GB/T 14926.57-2008 (GB/T14926.57-2008)
Description (Translated English): Laboratory animal -- Method for examination of canine parvovirus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B44
Classification of International Standard: 65.020.30
Word Count Estimation: 5,54
Date of Issue: 2008-12-03
Date of Implementation: 2009-03-01
Older Standard (superseded by this standard): GB/T 14926.57-2001
Quoted Standard: GB/T 14926.50; GB/T 14926.54
Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the canine parvovirus (CPV) detection methods, reagents. This section applies to dogs CPV testing.

GB/T 14926.57-2008: Laboratory animal -- Method for examination of canine parvovirus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Method for examination of canine parvovirus ICS 65.020.30 B44 National Standards of People's Republic of China Replacing GB/T 14926.57-2001 Animal canine parvovirus detection method Posted 2008-12-03 2009-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods. GB/T 14926.57-2001 "animal canine parvovirus detection method," Since the implementation of this section instead. This part of the main technical differences compared with the GB/T 14926.57-2001 as follows. Ability a) "3 principles" in "or under the CPV under certain conditions, to pig erythrocyte agglutination, this agglutination of red blood cells may Be inhibited by specific antibodies principles detection canine serum antibody CPV "to" under certain conditions or in accordance with CPV, Agglutinate pig or rhesus monkey red blood cells, this ability agglutination of red blood cells can be inhibited by specific antibodies principles detection dog blood Qing CPV antibody "; b) "4.1.6 pig erythrocytes" was changed to "4.1.6 pig or rhesus erythrocytes"; c) "6 results found" an increase in the "base-level 6.3 canine population of antibody passing rate of antibody qualified rate = (the subject animals Antibody-positive/is the total number of seized animals) × 100%. Ground-level pass rate of antibody dog \u200b\u200bpopulation greater than or equal than 70% penalty For the dog population qualified immunity "content. This part of the National Standardization Technical Committee of experimental animals and focal points. This part of the National Standardization Technical Committee responsible for drafting the experimental animals. The main drafters of this section. TIAN Ke-gong, He contention, Fan Wei. This part of the standard replaces the previous editions are. --- GB/T 14926.57-2001. Animal canine parvovirus detection method

1 Scope

GB/T 14926 provisions of this part of the canine parvovirus (CPV) testing methods, reagents and the like. This section applies to dogs CPV detection.

2 Normative references

The following documents contain provisions which, through reference GB/T 14926 in this section constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. ELISA GB/T 14926.50 Experimental Animals GB/T 14926.54 animal hemagglutination inhibition test Principle 3 According to the principles of immunology, antigen detection using CPV CPV canine serum antibody; or under the CPV under certain conditions, could agglutinate pig Or rhesus monkey red blood cells, this ability agglutination of red blood cells can be inhibited by specific antibodies principles detection canine serum CPV antibodies.

4 Reagents and equipment

4.1 Reagents 4.1.1 ELISA antigen 4.1.1.1 Specific Antigen CPV infection FK81 cells, 3d ~ 5d after inoculation, when the lesion reached +++ ~ ++++ (- negative; + Light; + in; +++ Weight; ++++ serious, when the same below) harvest. After three freeze-thaw or sonication, cell debris was removed by low speed centrifugation, supernatant was After ultracentrifugation concentrated into ELISA antigen. 4.1.1.2 normal antigen After freezing and thawing broken by low-speed centrifugation to remove cell debris and the supernatant obtained FK81 cells. 4.1.2 hemagglutinin CPV inoculated FK81 cells, cultured 3d ~ 5d, when the disease reaches +++ ~ ++++ harvest. Freezing and thawing three or ultrasonic treatment After low-speed centrifugation to remove cell debris, the supernatant was saved after dispensing cold. 4.1.3 positive serum CPV or experimentally infected dogs naturally infected serum. 4.1.4 negative serum No CPV infection without immune dog serum. 4.1.5 conjugate Horseradish peroxidase-labeled goat anti-dog or rabbit IgG antibody. 4.1.6 pig or rhesus erythrocytes. 4.2 Equipment 4.2.1 microplate reader. 4.2.2 37 ℃ incubator or water bath. ......

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