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GB/T 14926.46-2008 English PDF

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Q: Should I purchase the latest version GB/T 14926.46-2008?

Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 14926.46-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

US$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 14926.46-2008: Laboratory animal -- Method for examination of leptospira spp
Status: Valid

GB/T 14926.46: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 14926.46-2008	169	Add to Cart	3 days	Laboratory animal -- Method for examination of leptospira spp	Valid
GB/T 14926.46-2001	199	Add to Cart	2 days	Laboratory animal -- Method for examination of Leptospira sp	Obsolete

Similar standards

GB/T 43051 GB/T 39760 GB/T 35823 GB/T 14926.57 GB/T 14926.58 GB/T 14926.56

Basic data

Standard ID: GB/T 14926.46-2008 (GB/T14926.46-2008)
Description (Translated English): Laboratory animal -- Method for examination of leptospira spp
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B44
Classification of International Standard: 65.020.30
Word Count Estimation: 7,746
Date of Issue: 2008-12-03
Date of Implementation: 2009-03-01
Older Standard (superseded by this standard): GB/T 14926.46-2001
Quoted Standard: GB/T 14926.42; GB/T 14926.43; GB/T 14926.50
Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies a method for detecting animal leptospirosis. This section applies to the detection of canine leptospirosis.

GB/T 14926.46-2008: Laboratory animal -- Method for examination of leptospira spp

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Method for examination of leptospira spp ICS 65.020.30 B44 National Standards of People's Republic of China Replacing GB/T 14926.46-2001 Leptospirosis Laboratory Animal Detection Posted 2008-12-03 2009-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods. Since the implementation of this section instead of GB/T 14926.46-2001 "animal leptospirosis detection methods." This part of the main technical differences compared with the GB/T 14926.46-2001 as follows. a) the principle part of the increase microscopic agglutination test principle; b) the original standard "6.1 tube agglutination test" to "microscopic agglutination test"; "6.1.2.2 Table 1 Quantitative Leptospira Significant agglutination test method of operation "to" Leptospira quantitative microscopic agglutination test methods of operation "; c) modify all the content on the original standard ELISA test; d) increase the "7 results judgment" in the original standard. This part of the National Standardization Technical Committee of experimental animals and focal points. This part of the National Standardization Technical Committee responsible for drafting the experimental animals. The main drafters of this section. Fan Wei, TIAN Ke-gong, He contention. This Part replaces the previous editions are. --- GB/T 14926.46-2001. Leptospirosis Laboratory Animal Detection

1 Scope

GB/T 14926 provisions of this part of the detection method of experimental animals leptospirosis. This section applies to the detection of canine leptospirosis.

2 Normative references

The following documents contain provisions which, through reference GB/T 14926 in this section constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB/T 14926.42 bacteriological detection of animal specimen collection GB/T 14926.43 animal bacteriological detection Staining, media and reagents ELISA GB/T 14926.50 Experimental Animals Principle 3 Leptospira antigen-specific antibody with the corresponding encounter occurred agglutination in the presence of a suitable electrolyte, such aggregation are generally required to use Dark-field microscopy. Leptospira antigen and serum to be tested in the specific antibody binding, the formation of antigen-antibody complex. This antigen-antibody complex is still guaranteed Maintain their antigenic activity, it can be bound with a corresponding second antibody enzyme conjugates. In reaction catalyzed by the enzyme substrate to produce a colored substance. Proportional to the amount and depth of color of the reaction of the tested serum contained specific antibodies.

4 major equipment and materials

4.1 ordinary incubator. 4.2 stereomicroscope. 4.3 constant temperature water bath. 4.4 high-speed centrifuges. 4.5 ultrasonic cell pulverizer. 4.6 microplate reader. 4.7 micro pipette, capacity 5μL ~ 50μL and 50μL ~ 200μL.

5 medium and reagents

5.1 Canine Leptospira standard antigen. 5.2 standard positive serum. 5.3 standard negative serum. 5.4 saline. 5.5 microscopic agglutination Antigen. each group Leptospira strain was inoculated Korthof standard medium, 28 ℃ cultured 5d ~ 7d. Sampling for dark Field microscopy, for every 400 less than 50 times the field of view, there is no self-curing lively sports phenomenon, it could be used as an antigen. 5.6 ELISA antigen 5.6.1 Leptospira culture. each group Leptospira strain was inoculated Korthof standard medium, 28 ℃ cultured 5d ~ 7d. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 14926.46-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 14926.46-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 14926.46-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 14926.46-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 14926.46-2008?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 14926.46-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.