GB 5009.148-2014 PDF EnglishUS$70.00 · In stock · Download in 9 seconds
GB 5009.148-2014: National Food Safety Standard -- Determination of Free Gossypol in Vegetable Food Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid GB 5009.148: Historical versions
Similar standardsGB 5009.148-2014: National Food Safety Standard -- Determination of Free Gossypol in Vegetable Food---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.148-2014 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Determination of free gossypol in vegetable good ISSUED ON: FEBRUARY 04, 2015 IMPLEMENTED ON: MAY 01, 2015 Issued by: National Health and Family Planning Commission of PRC Table of ContentsForeword ... 3 1 Scope ... 4 2 Principle ... 4 3 Reagents and materials ... 4 4 Instrument and equipment ... 5 5 Analytical procedures ... 5 6 Expression of analysis results ... 6 7 Precision ... 7 8 Others ... 7 Appendix A Standard solution of gossypol and sample spiked chromatogram 8 National food safety standard - Determination of free gossypol in vegetable good1 ScopeThis standard specifies the method for determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material. This standard applies to the determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material.2 PrincipleThe free gossypol in vegetable oil was extracted by absolute ethanol and detected by high performance liquid chromatography. The retention time of the chromatographic peak is qualitatively determined, and it is quantified by external standard method. The free gossypol in the water-soluble liquid sample using cottonseed cake as the raw material is extracted by the use of anhydrous ether, concentrated to dryness, then dissolved in ethanol, detected by high performance liquid chromatography. The retention time of the chromatographic peak is qualitatively determined, and it is quantified by external standard method.3 Reagents and materialsNote: Unless otherwise specified, the reagents used in this method are all analytically pure; the water is the level 1 water as specified in GB/T 6682. 3.1 Reagents 3.1.1 Phosphoric acid (H3PO4). 3.1.2 Anhydrous ethanol (CH3CH2OH). 3.1.3 Acetone (C3H6O). 3.1.4 Nitrogen (N2). 3.1.5 Methanol (CH3OH). upper layer of ether. Use nitrogen to blow it dry. Use 1.0 mL of absolute ethanol to make its volume reach to the mark. Make it pass through a 0.45 μm filter membrane, which is the sample solution. 5.2 Chromatographic determination 5.2.1 Reference chromatographic conditions Chromatographic column: C18 column, 250 mm × 4.6 mm, 5 μm, or a column with equivalent performance. Mobile phase: Methanol: phosphoric acid solution = 85: 15. Flow rate: 1.0 mL/min. Column temperature: 40 °C ± 1 °C. Measurement wavelength: 235 nm. Sample injection volume: 10 μL. 5.2.2 Drawing standard curve Inject the standard working solution of gossypol into the high performance liquid chromatograph and record the peak height or peak area. Draw a standard curve with the peak height or peak area as the ordinate and the concentration of the standard working solution of gossypol as the abscissa (see Figure A.1 in Appendix A for the liquid chromatogram of the standard solution). 5.2.3 Specimen measurement Inject the specimen solution into the high performance chromatograph. Record the peak height or peak area. Calculate the concentration of gossypol in the test solution based on the standard curve (see Figure A.2 in Appendix A for the sample spiked liquid chromatogram).6 Expression of analysis resultsThe free gossypol content in specimen 5.1.1 is calculated according to formula (1): Where: X - The content of gossypol in the specimen, in milligrams per kilogram ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. |
