GB 23200.17-2016 English PDFUS$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 23200.17-2016: Food safety national standard -- Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography Status: Valid
Basic dataStandard ID: GB 23200.17-2016 (GB23200.17-2016)Description (Translated English): Food safety national standard -- Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography Sector / Industry: National Standard Classification of Chinese Standard: G25 Word Count Estimation: 10,113 Date of Issue: 2016-12-18 Date of Implementation: 2017-06-18 Older Standard (superseded by this standard): NY/T 1649-2008 Regulation (derived from): State Health Commission, Ministry of Agriculture, Food and Drug Administration Notice No. 16 of 2016 Issuing agency(ies): National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration GB 23200.17-2016: Food safety national standard -- Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Food safety national standard - Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography GB National Standards of People's Republic of China Instead of NY/T 1649-2008 National standards for food safety Determination of Thiabendazole Residues in Fruits and Vegetables Liquid chromatography National food safety standards- Determination of thiabendazole residue in fruits and vegetables Liquid chromatography 2016-12-18 Release.2017-06-18 Implementation National Health and Family Planning Commission of the People 's Republic of China Issued by the Ministry of Agriculture of the People 's Republic of China State Administration of Food and Drug Administration ForewordThis standard replaces NY/T 1649-2008 "Determination of thiabendazole residues in fruits and vegetables by high performance liquid chromatography". This standard and NY/T 1649-2008 compared to the main changes are as follows. - the standard name has been modified to increase the national standard for food safety; - modified in accordance with the format of the food safety standard. - normative reference documents to increase the GB 2763 "maximum pesticide residues in food" standard; - the preparation of the sample to increase the sampling site requirements and refine the requirements of the preparation of the sample; - Increased precision requirements. National standards for food safety Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography1 ScopeThis standard specifies the method for the determination of thiabendazole residues in vegetables and fruits by high performance liquid chromatography (HPLC). This standard applies to the determination of thiabendazole residues in vegetables and fruits.2 normative reference documentsThe following documents are indispensable for the application of this document. For dated filing applications, only the dated edition applies to this document. For undated references, the latest edition (including all modifications) applies to this document. GB 2763 National Standard for Food Safety - Maximum Residue Limit of Pesticides in Foodstuffs GB/T 6682 Analytical laboratory water specifications and test methods3 principleSamples of thiabendazole in the sample after extraction, according to thiophanate in acidic conditions dissolved in water, alkaline conditions dissolved in the principle of ethyl acetate, Purification, and then by the reverse phase chromatography, UV detector 300nm detection, according to the retention time qualitative, external standard method quantitative.4 Reagents and MaterialsUnless otherwise stated, only reagents identified as analytical reagents and water at GB/T 6682 level were used in the analysis. 4.1 Reagents 4.1.1 Methanol (CH3OH), chromatographic pure. 4.1.2 Ethyl acetate (CH3COOC2H5). 4.1.3 Sodium chloride (NaCl). 4.1.4 anhydrous sodium sulfate (Na2SO4). 650 ℃ burning 4 h, the dryer to save. 4.2 solution preparation 4.2.1 hydrochloric acid solution (0.1 mol/L). absorb 8.33 mL of hydrochloric acid, water volume to 1 L. 4.2.2 Sodium hydroxide solution (1.0 mol/L). Weigh 40 g of sodium hydroxide, dissolve in water and set to 1 L. 4.3 standards Thiabendazole (CAS 148-79-8). purity greater than 99%. 4.4 standard solution preparation Standard storage solution (100 mg/L). accurately weighed thymidazole 0.0100 g, dissolved in methanol, the volume to 100 mL, set 4 ℃ preservation, Valid for 3 months.5 instruments and equipment5.1 High Performance Liquid Chromatograph with UV Detector. 5.2 Analysis of balance. 0.01 g and 0.1 mg. 5.3 Tissue crusher. 5.4 Rotary Evaporator. 5.5 Mechanical reciprocating oscillator. 5.6 Buchner funnel.6 sample preparationSampling samples of vegetables and fruit samples according to GB 2763-2014 Appendix A sampling, for the smaller individual samples, after sampling the whole For the individual large uniform sample can be split on the axis of symmetry or symmetry or cut into small pieces of post-processing; for slender, flat Flat or component content in the various parts of the sample can be cut in different parts of small pieces or cut into small pieces or processing; take the sample after the chopped, Fully mixed, with a quartile sampling or directly into the tissue crusher mashed into homogenate. The homogenate was placed in a polyethylene bottle at -16 ° C to -20 ° C Save under the file.7 Analysis steps7.1 Extraction and purification Weigh 10 g sample, accurate to 0.01 g, into a 250 mL stoppered flask, add 40 mL of methanol, homogenize 1 min, Shaking on the shaker for 20 min, the Buchner funnel was filtered and the residue was washed twice with the appropriate amount of methanol. The filtrate was combined in a 150 mL pear flask. 50 ℃ under reduced pressure evaporated to the remaining 5 mL ~ 10mL, with 20 mL hydrochloric acid solution into the 250 mL separatory funnel, add 20 mL of ethyl acetate vibration Swing, stand still, ethyl acetate layer and then 20 mL hydrochloric acid solution extraction time. The combined aqueous phase was adjusted to pH 8 to 9 with sodium hydroxide solution and 4 g was added Sodium chloride, into the 250 mL separatory funnel, with 40 mL of ethyl acetate were extracted 2 times, combined with ethyl acetate, dehydrated by anhydrous sodium sulfate, At 50 ° C under reduced pressure rotary evaporation near dry, the residue dissolved with mobile phase and volume to 5 mL, filtered through the 0.45μm filter to be tested. 7.2 Liquid Chromatographic Reference Conditions Detector. UV detector. Column. C18, 4.6 x 250 mm (5 μm) or equivalent. W = (1) B. Mobile phase. methanol water = 50 50. Flow rate. 1.0 mL/min. Detection wavelength. 300 nm. Column temperature. room temperature. Injection volume. 10 μL. 7.3 Standard working curve Absorb the standard stock solution 0 mL, 0.1 mL, 0.5 mL, 1 mL and 2 mL, with the mobile phase volume to 10 mL, this standard series of quality The concentrations of 0 mg/L, 1.00 mg/L, 5.00 mg/L, 10.0 mg/L and 20.0 mg/L were used to determine the peak area as the ordinate and the corresponding standard The mass concentration of the liquid is the abscissa, the standard curve is drawn, the regression equation and the correlation coefficient are obtained. 7.4 determination The standard working solution and the test solution were injected into the high performance liquid chromatography, to retain the time qualitative, to be measured peak area into the standard Quasi-curve in the quantitative, the sample concentration of thiabendazole concentration should be in the standard working curve mass concentration range. At the same time do blank test. 8 Results calculation. The residual amount of thiabendazole in the sample is expressed in terms of mass fraction w, expressed in milligrams per kilogram (mg/kg), calculated according to formula (1) × V Where. - The mass concentration of thiabendazole in the sample solution is calculated from the standard curve in milligrams per liter (mg/L); V-final volume, in milliliters (mL); M-sample mass in g (g). The calculated results shall be deducted from the blank values and the calculated results shall be expressed as the arithmetic mean of the two independent determinations obtained under reproducible conditions. Leave two valid digits.9 precisionThe ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record A requirements. The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record B requirements. 10% limit The limit method of this standard method is 0.05mg/kg. 11 Chromatogram. Thiabendazole standard solution map shown in Figure 1. Figure 1 1.0mg/L thiabendazole standard solution map shown in FigureAppendix A(Normative appendix) Laboratory repeatability requirements Table A.1 Laboratory repeatability requirements Measured component content Mg/kg Precision 0.001 36 > 0.01 > 1 14Appendix B(Normative appendix) Inter-laboratory reproducibility requirements Table B.1 Inter-laboratory reproducibility requirements Measured component content Mg/kg Precision 0.001 54 > 0.01 > 1 19 ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 23200.17-2016_English be delivered?Answer: Upon your order, we will start to translate GB 23200.17-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 23200.17-2016_English with my colleagues?Answer: Yes. 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