GB 1886.257-2016 English PDF

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GB 1886.257-2016: National Food Safety Standard -- Food Additives -- Lysozyme
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 1886.257-2016	199	Add to Cart	3 days	National Food Safety Standard -- Food Additives -- Lysozyme	Valid

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Basic data

Standard ID: GB 1886.257-2016 (GB1886.257-2016)
Description (Translated English): National Food Safety Standard -- Food Additives -- Lysozyme
Sector / Industry: National Standard
Classification of Chinese Standard: X40
Word Count Estimation: 10,124
Date of Issue: 2016-08-31
Date of Implementation: 2017-01-01
Regulation (derived from): Announcement of the State Administration of Public Health and Family Planning 2016 No.11
Issuing agency(ies): National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB 1886.257-2016: National Food Safety Standard -- Food Additives -- Lysozyme

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Food additive - Lysozyme) National Standards of People's Republic of China National standards for food safety Food Additives Lysozyme 2016-08-31 released 2017-01-01 Implementation People's Republic of China National Health and Family Planning Commission released National standards for food safety Food Additives Lysozyme

1 Scope

This standard applies to egg white as raw material, the extraction, refining and other processes obtained by the food additives lysozyme.

2 technical requirements

2.1 sensory requirements Sensory requirements shall comply with the requirements of Table 1. Table 1 sensory requirements project Claim Solid liquid Testing method Color white to pale yellow light yellow to dark brown State powder liquid Place the appropriate amount of sample evenly in a beaker or white porcelain dish, in nature Observe the color and state under light 2.2 physical and chemical indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2 Physical and chemical indicators project index Solid liquid Testing method Enzyme activity/[U/mg (mL)] in line with labeling values Appendix A, A.2 Moisture, w /% ≤ 6 - GB 5009.3 Ash, w /% ≤ 1.5 0.3 GB 5009.4 pH 3.0 to 7.0 Appendix A, A.3 Lead (Pb)/(mg/kg) ≤ 2.0 GB 5009.12 Total arsenic (in As)/(mg/kg) ≤ 1.0 GB 5009.11 2.3 Microbiological indicators Microbiological indicators should be in accordance with Table 3. Table 3 Microbiological indicators Item Index Test Method Total number of colonies/[CFU/g (mL)] ≤ 50000 GB 4789.2 Mold and yeast/[CFU/g (mL)] ≤ 100 GB 4789.15 Escherichia coli/[MPN/g (mL)] < 3.0 GB 4789.38 Salmonella/25g (mL) Not detected GB 4789.4 Staphylococcus aureus/25g (mL) shall not be detected GB 4789.10 Qualitative test

Appendix A

Testing method A.1 General provisions In addition to the provisions of this standard, the purity of the reagents used should be above the analytical level, the use of standard titration solution, impurity determination standard solution, Preparation and preparation of products should be prepared according to GB/T 601, GB/T 602, GB/T 603, the experimental water should be consistent with GB/T 6682 in the three water Provisions. The solution used in the test refers to the aqueous solution when it is not specified in the formulation of the solvent. A.2 Determination of enzyme activity A.2.1 Methodology Lysozyme hydrolyzes the cell wall of the bacteria, causing the dissolution of the Micrococcus luteus and causing the decrease in the absorbance of the solution. A lysozyme activity unit was defined as 25 ° C under pH 6.2, using a rattanin micrococcus suspension at 450 nm The amount of lysozyme required for the change in absorbance is 0.001. A.2.2 Reagents and materials A.2.2.1 Micrococcus luteus. ATCC4698 or CICC10680. A.2.2.2 0.1 mol/L phosphate buffer. pH 6.2. 11.70 g of sodium dihydrogenphosphate (NaH2PO4 2H2O), 7.86 g of disodium hydrogen phosphate (Na2HPO412H2O) and 0.372 g Ethylenediamine tetraacetic acid disodium (EDTA-2Na) in sterile water and diluted to 1000 mL. Adjust the buffer solution to pH 6.2 ± 0.1. Note. Check the pH with a small buffer solution to avoid contamination of the buffer solution. If necessary, by adding more sodium dihydrogen phosphate solution or disodium hydrogen phosphate Solution to adjust the pH. A.2.2.3 lysozyme standard. egg white lysozyme. A.2.2.4 Substrate solution. 50 mL of the suspension of Microcystis aeruginosa was prepared with phosphate buffer. The substrate was incubated at 37 ° C for 30 min before use. The substrate solution can be stabilized at room temperature for 2 h. The pH of the photometer was adjusted with the phosphate buffer and the absorbance of the substrate solution was measured. The reading should be 0.70 ± 0.1 at 450 nm. A.2.3 Instruments and equipment A.2.3.1 Spectrophotometer. Accuracy ± 0.001. A.2.3.2 pH meter. Note. The utensils used should be kept sterile to ensure the cleanliness of the working environment. A.2.4 Analysis steps A.2.4.1 Preparation of sample solution Accurately weighed 100mg ± 0.1mg sample, placed in 50mL volumetric flask, with about 25mL phosphate buffer stirring dissolved and diluted Constant volume, full mixing. And then transferred 3mL of the above sample preparation solution to 100mL volumetric flask, with phosphate buffer stirring dissolved and dilute Release capacity. A.2.4.2 Preparation of standard solutions Accurately weighed 50 mg of egg white lysozyme standard in a 50 mL volumetric flask, stirred and diluted with about 25 mL of phosphate buffer (If necessary, freeze the solution for later determination). Transfer 3 mL of the above standard preparation solution to 100 mL capacity The flask was stirred and dissolved with phosphate buffer and diluted. A.2.4.3 Determination Three standard solutions and three sample solutions were taken. 25 ° C at room temperature, 1cm cuvette into the spectrophotometer, with phosphate buffer to adjust the absorbance zero. Suction 2.9 mL of substrate Solution in the cuvette, the initial absorbance at 450nm should be 0.70 ± 0.10,3min within the initial absorbance value change should be less than or equal to 0.003, before you can start to determine. Add 0.1 mL of the standard solution to the substrate solution and mix well. Record the change in absorbance value for 3 min The absorbance value is recorded every 15s. The change in absorbance per minute should be 0.03 to 0.08, if not in the required range to adjust the sample solution concentration. Repeat the test sample solution. After 1 min reaction, the initial 1 min reading was ignored. A.2.4.4 Calculation of results Enzyme activity X, calculated according to formula (A.1). X = (A1-A2) 2 × m × 0.001 (A.1) Where. A1 - the absorbance of the sample at 450 nm for 1 min; A2 - absorbance at 450 nm at 3 min; m --- for the analysis of the sample preparation solution in the amount of lysozyme in milligrams (mg); 2 - the time taken to obtain 1min and 3min absorbance readings in minutes (min); 0.001 --- The value of the absorbance decreases per minute caused by unit lysozyme. The results of the test are based on the arithmetic mean of the parallel measurement results. The absolute difference between the two independent determinations obtained under repeatability conditions The value is not greater than 10% of the arithmetic mean. A.3 Determination of pH A.3.1 Instruments A.3.1.1 Acidity meter. Accuracy 0.01pH, with glass electrode and calomel electrode (or composite electrode). A.3.2 Analysis steps Use the instrument manual to debug and calibrate the acidity meter. Preparation of 15g/L lysozyme solution with sterile water (liquid product direct determination). Rinse the electrode probe with water and gently suck it with filter paper Dry, the electrode into the liquid to be measured, adjust the temperature regulator, so that the instrument indicates the temperature and the temperature of the same test fluid, after the stability of the reading. The result is expressed to a decimal. ......

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