GB 10794-2009 English PDFUS$399.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 10794-2009: Food additive -- L-lysine monohydrochloride Status: Obsolete GB 10794: Historical versions
Basic dataStandard ID: GB 10794-2009 (GB10794-2009)Description (Translated English): Food additive -- L-lysine monohydrochloride Sector / Industry: National Standard Classification of Chinese Standard: X42 Classification of International Standard: 67.220.20 Word Count Estimation: 10,198 Date of Issue: 2009-01-19 Date of Implementation: 2009-08-01 Older Standard (superseded by this standard): GB 10794-1989 Quoted Standard: GB/T 601; GB/T 5009.11-2003; GB/T 5009.12; GB/T 6682 Regulation (derived from): Announcement of Newly Approved National Standards No. 2 of 2009 (total 142) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This Chinese standard specifies the L-lysine hydrochloride requirements, test methods, inspection rules and signs, packaging, transportation, storage and shelf life. This standard applies to starch or sugar feedstock, purification obtained by the fermentation of L-lysine hydrochloride products. GB 10794-2009: Food additive -- L-lysine monohydrochloride---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Food additive.L-lysine monohydrochloride ICS 67.220.20 X42 National Standards of People's Republic of China Replacing GB 10794-1989 Food additives L- lysine hydrochloride Posted 2009-01-19 2009-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released Foreword4.2 This standard is mandatory, the remaining provisions are recommended. The standard physical and chemical indicators refer to the United States, "Food Chemicals Codex" (FCCⅤ) and Japan Kimisada book eighth edition of the corresponding technical requirements. This standard replaces GB 10794-1989 "Food Additive L- lysine monohydrochloride." This standard compared with GB 10794-1989 main changes are as follows. --- Tightened specific rotation requirements; --- Predetermined content target range; --- Of lead indicators instead of heavy metal requirements; --- Increased salt indicators; --- Analysis adjusted accordingly. Appendix A of this standard is a normative appendix. This standard by the National Standardization Technical Committee on Food Additives proposed. This standard by the National Standardization Technical Committee on Food Additives. This standard was drafted. Chinese Food Fermentation Industry Research Institute, Guangdong Xinghu Lake Biological Technology Co., Ltd .. The main drafters. Zhang Wei, Wei basket loose, Luqin Ying, Chu Chang Xia, Guo Xinguang, Zheng Jian Ning. This standard replaces the standards previously issued as follows. --- GB 10794-1989. Food additives L- lysine hydrochloride1 ScopeThis standard specifies the L- lysine hydrochloride requirements, test methods, inspection rules and signs, packaging, transportation, storage and shelf life. This standard applies to the starch or sugar feedstock, obtained by fermentation of purified L- lysine hydrochloride products.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Preparation of GB/T 601 chemical reagent standard titration solution Determination of total arsenic and inorganic arsenic GB/T 5009.11-2003 Determination of GB/T 5009.12 of lead in food GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) 3 chemical name, molecular formula, structural formula, relative molecular mass 3.1 Chemical Name. L-2,6- diamino-hexanoic acid salt. 3.2 Molecular formula. C6H14N2O2 · HCl. 3.3 Molecular Weight. 182.65. 3.4 Structure. NH2 (CH2) 4CCOOH · HCl H NH24 Requirements4.1 Sensory requirements White crystal or crystalline powder; odorless. Soluble in water, very slightly soluble in ethanol, insoluble in ether; no visible impurities. 4.2 Physical and Chemical Requirements Shall comply with the requirements of Table 1. Table 1 L- lysine hydrochloride Physicochemical Requirements Project Requirements Specific rotation [α] 20D + 20.3o ~ + 21.5o Content (dry matter basis)/98.5 to 101.5% Transmittance /% ≥ 95.0 Loss on drying /% ≤ 1.0 pH 5.0 ~ 6.0 Ash /% ≤ 0.2 Lead (Pb)/(mg/kg) ≤ 5 Arsenic (As)/(mg/kg) ≤ 1 Salt /% ≤ 0.025 Test methodsThis standard is used in the water, did not specify when the other requirements, refer to comply with GB/T 6682 the requirements. This standard reagents, when not stated specifications, refer to AR (AR). If the special requirements otherwise expressly provided. This standard solution in a solvent which does not indicate when formulated with, refers to an aqueous solution. 5.1 sensory test The sample is spread on a white paper, the naked eye, sniffing, the results should be consistent with the provisions of 4.1. 5.2 Specific rotation 5.2.1 Instruments Automatic polarimeter. 5.2.2 Reagents Hydrochloric acid solution (6mol/L). 5.2.3 Analysis of step Weigh dried at 105 ℃ to constant weight of the sample 5g (accurate to 0.0001g), was dissolved in hydrochloric acid solution 6mol L /, and transferred to 50mL Flask, add 6mol L hydrochloric acid solution/close to the mark, the temperature of the solution was adjusted to 20 ℃, with 6mol/L hydrochloric acid solution to volume 50mL, mix well. 2dm with a long optical tube measuring its optical rotation. Also recorded sample liquid temperature. 5.2.4 Calculation The use of the sodium D line spectrum, 2dm optical tube when the sample was measured at 20 ℃, according to equation (1) calculate the specific rotation of the sample. (1) Where. Specific rotation [α] 20D --- 20 ℃ when the sample in degrees (o); α1 --- 20 ℃ sample solution when measured optical rotation in degrees (o); 2 --- Polarimetry length in decimeters (DM); (2) Where. 2 --- Polarimetry length in decimeters (DM); Specific rotation [α] 20D --- 20 ℃ when the sample in degrees (o); 0.02 --- L- lysine monohydrochloride temperature correction coefficient. Results reserved to one decimal place. 5.2.5 allowable difference The measurement results of the same sample, relative to the average deviation of not more than 0.3%. 5.3 Content 5.3.1 Reagents and solutions 5.3.1.1 formic acid. 5.3.1.2 glacial acetic acid. 5.3.1.3 mercuric acetate - acetic acid solution (6%). Weigh 6.0g mercuric acetate, acetic acid plus 100mL dissolve, and mix. 5.3.1.4 α- naphthol benzhydrol indicator solution (0.2%). Weigh 0.2g phenyl α- naphthol methanol, add 100mL glacial acetic acid dissolve, mix, prepared use. 5.3.1.5 perchlorate standard solution (0.1mol/L). according to GB/T 601 Preparation and calibration. 5.3.2 Analysis of step Weigh dried at 105 ℃ to constant weight of the sample 0.2g (accurate to 0.0001g), add formic acid 3mL, dissolved, ice acetic acid 50mL, Mercuric acetate - acetic acid solution (5.3.1.3) 5mL, then add 10 drops of indicator solution (5.3.1.4), calibrated with standard perchloric acid solution (5.3.1.5) titration until the solution was green, the recording volume perchloric acid standard solution consumed at the same time make a blank titration test. NOTE. If the temperature difference between the calibration sample is titrated with perchloric acid solution at more than 10 ℃, the concentration shall be re-calibrated perchloric acid solution, if a sample is titrated with calibrated high When the temperature difference between the acid solution does not exceed 10 ℃, according to equation (4) correcting the concentration of perchloric acid solution. (4) Where. Perchloric acid concentration of the solution during the titration sample --- Ba, units per liter (mol/L); 0 --- Ba perchloric acid concentration of the solution during calibration unit per liter (mol/L); 0.0011 --- expansion coefficient of acetic acid; 5.3.3 Calculation L- lysine hydrochloride content according to the formula (5) Calculated. X1 = 1 Ba (Vp - Vp 0) × 0.09132 100 (5) Where. Content X1 --- L- lysine hydrochloride,%; Ba 1 --- standard solution of perchloric acid equivalent concentration, in units of moles per liter (mol/L); Vp --- sample titration consumed perchloric acid standard titration solution volume in milliliters (mL); V0 --- blank titration consumed perchloric acid standard titration solution volume in milliliters (mL); 0.09132 --- mass L- lysine monohydrochloride and 1mmol perchloric acid equivalent in grams, in units of grams (g); Results reserved to one decimal place. 5.3.4 allowable difference The measurement results of the same absolute difference between the two samples shall not exceed 0.2% of the arithmetic mean. 5.4 transmittance 5.4.1 Instruments 5.4.1.1 flask. 100mL. 5.4.1.2 spectrophotometer. 5.4.2 Analysis of step Weigh 5g sample (accurate to 0.01g), dissolved in water, dilute to 100mL, shake; with 1cm cuvette, with water as the blank, Determination of light transmittance of the sample solution at a wavelength of 430nm, the readings are recorded. 5.4.3 allowable difference The absolute difference between the same two samples of the test results shall not exceed 0.2% of the arithmetic mean. 5.5 Loss on drying 5.5.1 Instruments 5.5.1.1 electric oven. 5.5.1.2 Analytical balance. a sense of the amount of 0.1mg. 5.5.1.3 weighing bottle. 50mm × 30mm. 5.5.1.4 drier. with color gel desiccant. 5.5.2 Analysis of step Weigh the sample 2g (accurate to 0.0002g) has been drying to constant weight in the weighing bottle, placed in 105 ℃ ± 2 ℃ electric oven drying 3h, stamp removed, placed in the dryer, cooling 30min, weighing. 5.5.3 Calculation Loss on drying of the sample according to equation (6) Calculated. 100 (6) Where. X2 --- Loss on drying of the sample,%; 5.5.4 allowable difference The absolute difference between two samples of the same measurement result shall not exceed 1% of the arithmetic mean. 5.6.1 Instruments Acidity (pH meter). 5.6.2 Analysis of step Weigh the sample 5g (accurate to 0.02g), add water to dissolve 50mL, with acidimeter solution pH. 5.6.3 allowable difference The measurement results of the same sample twice the absolute difference does not exceed 0.02pH. 5.7 Ash 5.7.1 Instruments 5.7.1.1 muffle furnace. 550 ℃ ± 25 ℃. 5.7.1.2 porcelain crucible. 5.7.1.3 drier. with color gel desiccant. 5.7.2 Analysis of step To constant weight by burning crucible weighed sample 1g (accurate to 0.0001g), placed on a hot plate heated slowly, carefully charring cooling. plus 1mL ~ 2mL of concentrated sulfuric acid, heated until smoking, then move a muffle furnace, burning at 550 ℃ ± 25 ℃ 2h, until the furnace temperature dropped to 300 ℃ left Right, take out the crucible, covered, in a desiccator, cooled to room temperature, and weighed. Then move a muffle furnace burning 1h, remove, cooling, weighing, weight Repeat the above operation until constant weight. 5.7.3 Calculation Ash samples according to equation (7) Calculated. 100 (7) Where. Ash,% X3 --- sample; Results reserved to one decimal place. 5.7.4 allowable difference The absolute difference between two samples of the same measurement result shall not exceed 1% of the arithmetic mean. 5.8 Lead Weigh sample 1g (accurate to 0.01g), dissolved in water and dilute to 50mL, shake, not digested, as the test solution. The following press GB/T 5009.12 Determination. 5.9 Arsenic According to GB/T 5009.11-2003 second method. 5.10 salts 5.10.1 Reagents and solutions 5.10.1.1 Standard chloride solution. Weigh ammonium chloride 31.5mg, set 1000mL volumetric flask, add water to dissolve and dilute to the mark Degree, shake, that was the equivalent of 10μg per 1mL of NH4 + Solution. 5.10.1.2 sodium hydroxide solution (1mol/L). 5.10.1.3 magnesia. 5.10.1.4 hydrochloric acid solution. take 23.41mL amount of concentrated hydrochloric acid, diluted with water to 100mL. 5.10.1.5 alkaline potassium mercuric iodide test solution. take potassium iodide 10g, add water to dissolve 10mL, slowly added a saturated aqueous solution of mercury dichloride, With the added with stirring to the resulting red precipitate is no longer dissolved, the hydrogenation hydroxide 30g, dissolved, coupled with a saturated solution of mercury dichloride and an aqueous solution 1mL 1mL or more, and diluted with an appropriate amount of water to make 200mL, allowed to stand to precipitate, that is, too. The supernatant was poured from the upper application when used. 5.10.2 Instruments 5.10.2.1 retorts. 500mL. 5.10.2.2 Nessler colorimetric tube. 50mL. 5.10.3 analysis step Weigh sample 0.10g, home distillation flask, add ammonia-free distilled water 200mL, plus magnesium oxide 1g, heating distillation, distillate imported salt added Acid solution and 1 drop of ammonia-free distilled water 50mL 5mL Nessler colorimetric tube, up to 40mL distillate, the distillation was stopped, add sodium hydroxide test 5 drops of liquid, add ammonia-free distilled water to 50mL, plus alkaline potassium mercuric iodide test solution 2mL, shake, place 15min, such as color, with the standard ammonium chloride 2mL solution of the above process control solution comparison, not deeper color.6 Inspection rules6.1 Batch The same process, within a certain time interval, continuous production of homogeneous product batch. 6.2 Sampling 6.2.1 take samples in Table 2. Table 2 sampling table Batch Range/bag sample size/bag ≤25 3 26 ~ 1508 151 to 50013 > 50020 6.2.2 The sampling solder into each sample at 5/6, less than 100g sample extraction, batch extraction of the total sample volume of not less than 1kg. The pumping Samples taken rapid mixing, after shrinking by quartering points, respectively, into two dry, clean container, labeled. 1 part by physicochemical points Analysis, and 1 copy retained for future reference. 6.3 factory inspection Products manufactured prior to 6.3.1, according to the standard batch testing. 6.3.2 factory inspection items. Specific rotation, content, transmittance, loss on drying, pH, ash, salt. 6.4 Type inspection 6.4.1 Type inspection items. In addition to the factory inspection items, as well as identification tests, arsenic and lead. 6.4.2 product under normal production conditions, type of inspection every six months, any of the following circumstances, shall be. --- When normal production, such as raw materials, formulations or processes are major changes, it may affect product quality; --- Long-term products discontinued, resumed production; --- Factory inspection results are quite different from normal production time; --- Quality Supervision, Inspection agencies when requested. 6.5 determine the rules 6.5.1 When the test results, there is a test project failed, the batch of products should be re-extracted from the same amount twice retest samples to complex Test results shall prevail. If a failure, then the whole batch judged to be defective. 6.5.2 When the supply and demand sides of the product mass protest occurs, the arbitration unit selected by the mutual agreement, according to the standard retest. 7 signs, packaging, transportation, storage and shelf life 7.1 marks Food additives must be packaged logos and product brochures, signs can include. name, place of origin, name, health permits, production License number, specifications, production date, lot number or code, shelf life, etc., and clearly indicate the words "food additive" in the logo. 7.2 Packaging 7.2.1 product packaging shall be approved by the state, and in accordance with relevant hygiene standards of food packaging materials. 7.2.2 Packing. Inner packing tight seal can not be breathable packaging must not be contaminated. 7.3 Transport Products during transport shall not be mixed and carried toxic and hazardous substances and pollution, avoid the sun and rain and so on. 7.4 Storage The product should be stored in well-ventilated, clean, dry place, not with toxic, hazardous and corrosive and other substances exist. 7.5 Shelf Life From the date of manufacture, in the case of the above-stated storage conditions, original packaging intact, the shelf life of not less than six months.Appendix A(Normative) Identification Test L- lysine monohydrochloride A. Claim 1 amino acid A. 1.1 Reagents and solutions Ninhydrin solution (0.1%). HG3-984. A. 1.2 Analysis Steps a) a sample was weighed 0.1g (accurate to 0.01g), dissolved in water and diluted to 100mL; b) draw sample liquid [a)] 5mL, add 1mL ninhydrin solution, mixed, heated in a water bath 3min. A. 1.3 results to determine If the final solution was purple, it is recognized as an amino acid. A. Claim 2 chloride A. 2.1 Reagents and solutions A. 2.1.1 nitrate. A. 2.1.2 silver nitrate solution (0.1mol/L). Weigh 17.5g of silver nitrate, dissolved in water and diluted to 1000mL. A. 2.1.3 ammonia solution (10%). A. 2.2 Analysis Steps a) Weigh the sample 1g (accurate to 0.1g), dissolved in water; b) To the above solution [a)] was added silver nitrate solution (A.2.1.2) 5mL, and mix. A. 2.3 determination results If immediately generated a white emulsion precipitation, the precipitate insoluble in nitric acid, and slightly soluble in excess of ammonia, it is determined chlorides. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 10794-2009_English be delivered?Answer: Upon your order, we will start to translate GB 10794-2009_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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