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NY/T 2543-2014: PDF in English (NYT 2543-2014)
NY/T 2543-2014
NY
AGRICULTURAL INDUSTRY STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
ICS 65.080
B 10
Fertilizer synergist - Regulations of efficiency
experiment and assessment
ISSUED ON. MARCH 24, 2014
IMPLEMENTED ON. JUNE 01, 2014
Issued by. Ministry of Agriculture of PRC
Table of Contents
Foreword ... 3 
1 Scope ... 4 
2 Terms and definitions ... 4 
3 General requirements ... 6 
4 Plot experiment ... 8 
5 Demonstration experiment ... 9 
6 Assessment requirements ... 10 
7 Experiment report ... 11 
Appendix A (Normative) Fertilizer synergist - Requirements for conditional
culture experiment ... 12 
Appendix B (Normative) Fertilizer synergist - Requirements for pot experiment
... 16 
Appendix C (Normative) Fertilizer synergist - Requirements for experiment
record ... 18 
Fertilizer synergist - Regulations of efficiency
experiment and assessment
1 Scope
This standard specifies the relevant terms, experiment requirements and
contents, effect assessment, report writing and other requirements for the
efficiency experiment of the fertilizer synergist.
This standard applies to the assessment of the experiment results of urease
inhibitors and nitrification inhibitors.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Fertilizer synergist
The general term of urease inhibitors and nitrification inhibitors.
2.2
Urease inhibitors
A certain amount of material added to the urea. It reduces soil’s urease
activity and inhibits the urea’s hydrolysis process, to reduce of ammonia
volatilization loss of the amide nitrogen, thereby improving the fertilizer use
efficiency.
2.3
Nitrification inhibitors
A certain amount of material added to the ammonium nitrogen fertilizer. It
reduces the activity of soil’s nitrite bacteria, inhibits the process of conversion
of ammonium nitrogen to nitrate nitrogen, so as to reduce the loss of fertilizer
nitrogen, thereby improving the fertilizer use efficiency.
2.4
Urease-inhibition rate
The experiment treatment shall be designed according to the type of fertilizer
synergist, it shall also fully consider the factors such as the type of nitrogen
fertilizer, the amount of application and the uniformity of application of different
synergists.
3.3.1 The experiment shall be provided with at least 4 treatments.
- Blank control.
- Conventional fertilization.
- Fertilizers with fertilizer synergist which has iso-nutrient with the
conventional fertilization.
- Fertilizers with fertilizer synergist which has iso-nutrient with the
conventional fertilization reduced by 10%.
Note. The type of nitrogen fertilizer in conventional fertilization shall be the same
as that in the fertilizer synergist applied; the demonstration experiment may not
have a blank control.
3.3.2 If necessary, it may increase other experiment treatments.
- Fertilizers with fertilizer synergist which has iso-nutrient as the conventional
fertilization reduced by 20% (or higher).
- Recommended fertilizer with the best fertilizer synergist.
- The recommended best application amount of the fertilizer synergist and
the conventional fertilizer.
3.3.3 Except for the blank control, other experiment treatments shall specify the
time and method of fertilization, including the application rate of base fertilizer,
the application rate of top-dressing, the number of applications.
Note. Fertilization methods can be divided into spreading application, hole
application, strip application, spraying application, seed soaking, root irrigation,
root dipping, and so on.
3.3.4 Each treatment of plot experiment shall be arranged in a random block;
the number of repetitions shall not be less than 3.
3.4 Preparation for experiment
3.4.1 Selection of experiment site.
- It shall select an experiment sites with flat terrain, neat shape and relatively
uniform soil fertility level.
the plot for densely planted crops (such as rice, wheat, millet, etc.) should be
20 m2 ~ 30 m2; the area of the plot for cultivated crops (such as corn, sorghum,
cotton, tobacco, etc.) should be 40 m2 ~ 50 m2; the area of the plot for fruit trees
should be 50 m2 ~ 200 m2.
Note. If it is highly treated, the area of the plot should be smaller; if it is less
treated, the area of the plot should be larger. The area of the plot should be
relatively smaller in hills, mountains, and slopes; whilst the area of the plot
should be relatively larger in plains and flat land.
4.4 Requirements for plot shape
The shape of the plot shall generally be rectangular. When the area of the plot
is large, the aspect ratio is preferably (3 ~ 5) . 1; when the area of the plot is
small, the aspect ratio is preferably (2 ~ 3) . 1.
4.5 Requirements for experiment result
- Each plot shall be harvested separately to calculate yield.
- According to the statistical fertilizer & labor-saving conditions of the plot,
calculate the net income and output/input ratio.
- When analyzing the crop quality, it shall take sample in accordance with the
requirements for experiment method.
5 Demonstration experiment
5.1 Experiment content
The demonstration experiment is an effect experiment conducted on a widely
representative area of farmland, to demonstrate and assess the results of the
plot experiment.
5.2 Requirements for demonstration area
- The demonstration area for economic crop shall be not less than 3000 m2;
the demonstration area for control shall be not less than 500 m2.
- The demonstration area for field crop shall be not less than 10000 m2; the
demonstration area for control shall be not less than 1000 m2.
- For the demonstration experiments such as flowers, seedlings and lawns,
it shall consider their particularity, the experiment area shall be not less than
that of economic crops.
5.3 Requirements for experiment result
Appendix A
(Normative)
Fertilizer synergist - Requirements for conditional culture experiment
A.1 Experiment content
Use the conditional culture experiments to evaluate the inhibitory effects of
urease inhibitors and nitrification inhibitors on soil’s nitrogen transformation.
The soil water content is maintained within the range of 65% ~ 80% of the field
water holding capacity, the temperature of the constant temperature incubator
is (25 ± 2) °C.
A.1.1 Urease inhibitor experiment. Through measuring the amount of
in the soil at different time points, calculate the difference between each
treatment.
Note. Urease inhibitors inhibit soil’s urease activity for a period of time, delaying
the hydrolysis of urea in the soil; it has a higher content of amide nitrogen as
compared with the control, and a lower content of .
A.1.2 Nitrification inhibitor experiment. Through measuring the amount of
in the soil at different time points, calculate the difference between
each treatment.
Note. Nitrification inhibitors inhibit the activity of nitrite bacteria for a period of
time, delaying the conversion of to ; it has a higher content
of as compared with the control, and a lower content of as
compared with the control.
A.2 Treatment for experiment
A.2.1 The experiment shall have at least the following three treatments.
- Blank control.
- Iso-nutrient fertilizers.
- Iso-nutrient fertilizers, which are added with nutrient synergist.
A.2.2 If necessary, it may add other experiment treatments and control
treatment.
- Iso-nutrient fertilizer, which is added with different amounts of fertilizer
synergist.
A.5.4 Implementation steps
According to the experiment design requirements, determine the addition
amount of material required for the experiment treatment. Respectively weigh
40.0 g of soil and the corresponding experiment materials in a 500 mL beaker,
mix it uniformly, transfer them all into a 100 mL beaker, weigh them in turn and
mix them uniformly. According to the moisture content of soil, calculate the
amount of water to be added to each beaker, uniformly add it into the soil. After
using the sealing film to seal it, use a needle to puncture uniformly 5 vents,
place it in an incubator at 25 °C for culture, during which it shall timely with and
supplement soil moisture. According to the sampling interval requirements,
remove the beaker, pour all soils into a 500 mL beaker to mix it uniformly,
determine the content of or as well as the moisture
content of soil.
Note. It shall, according to the moisture content of the experimented soil,
respectively calculate the mass of the beaker that meets the moisture
requirements of the conditional culture, respectively supplement in time the soil
moisture according to the quality requirements (the moisture content of the
experimented material is considered same as the soil moisture content); the
soil sample and the experimented material may be diluted stepwise to mix it
uniformly.
A.5.5 Expression of results
A.5.5.1 Urease-inhibition rate is calculated based on mass fraction X1, the value
is expressed as a percentage, calculated according to formula (A.1).
Where.
c1 - The amount of soil (calculated by dry soil) treated with iso-
nutrient fertilizer, in milligrams per kilogram (mg/kg);
c2 - The amount of soil (calculated by dry soil) treated with iso-
nutrient fertilizer which is added with urease inhibitor, in milligrams per
kilogram (mg/kg).
A.5.5.2 Nitrification-inhibition rate is calculated by mass fraction X2, the value
is expressed as a percentage, calculated according to formula (A.2).
Appendix B
(Normative)
Fertilizer synergist - Requirements for pot experiment
B.1 Experiment content
The pot experiment is suitable for small-plot experiments, so as to more-
accurately assess the effect experiment of fertilizer synergists on inhibiting soil
ammonia transformation.
- By artificially controlling the experiment treatment and environmental
conditions, to ensure the experiment management consistency of soil
temperature, moisture, uniformity of experimented soil’s conditioner, crop
planting in the experiment vessel.
- The experimented soil in the pot experiment is an unnaturally structured
soil, some soil traits may change.
B.2 Experiment requirements
The experiment shall meet the following requirements, the others are
implemented in accordance with the requirements of 3.
B.2.1 Collection and preparation of experimented soil
- Determination of collection sites and number of sampling points of soil shall
consider the representativeness of the farming area; the sampling depth is
generally 0 cm ~ 20 cm. The collection and preparation processes of soil
shall avoid contamination.
- Make the collected soil pass through a 5 mm aperture sieve, mix it uniformly.
- For the prepared soil under experiment, indicate the soil name, collection
site, collection time, and main soil traits.
B.2.2 Selection of pot
- Pots may be glass pots, enamel iron pots, clay pots and plastic pots.
- The pot size may be selected from 20 cm x 20 cm, 25 cm x 25 cm, 30 cm
x 30 cm.
B.2.3 Each treatment shall be randomly arranged, the number of repetition is
not less than 4.
......

BASIC DATA
Standard ID NY/T 2543-2014 (NY/T2543-2014)
Description (Translated English) Fertilizer synergist. Regulations of efficiency experiment and assessment
Sector / Industry Agriculture Industry Standard (Recommended)
Classification of Chinese Standard B10
Classification of International Standard 65.080
Word Count Estimation 12,173
Date of Issue 2014/3/24
Date of Implementation 2014/6/1
Drafting Organization ?Ministry of Agriculture fertilizer registration review committee
Administrative Organization ?PRC Ministry of Agriculture
Regulation (derived from) Ministry of Agriculture Bulletin No. 2081
Issuing agency(ies) Ministry of Agriculture of the People's Republic of China
Summary This standard specifies the relevant terms of fertilizer synergist effect test, test requirements and content, effect evaluation, report writing and other requirements. This standard applies to urease inhibitor and nitrification inhibitor test results eva