GB/T 11446.10-2013 (GB/T11446.10-2013, GBT 11446.10-2013, GBT11446.10-2013) & related versions
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GB/T 11446.10-2013
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 31-030
L 90
Replacing GB/T 11446.10-1997
Test method for total bacterial count in
electronic grade water by membrane filters
ISSUED ON. DECEMBER 31, 2013
IMPLEMENTED ON. AUGUST 15, 2014
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Normative references ... 5
3 Terms and definitions ... 5
4 Methodology ... 6
5 Interference factors ... 6
6 Reagents ... 6
7 Measuring instruments and equipment ... 7
8 Operation procedures ... 8
9 Measurement result calculation ... 8
10 Report ... 9
Foreword
The estimated structure of GB/T 11446 is as follows.
- GB/T 11446.1 Electronic grade water;
- GB/T 11446.2 (To be determined);
- GB/T 11446.3 Generic rules for test methods of electronic grade water;
- GB/T 11446.4 Test methods for resistivity of electronic grade water;
- GB/T 11446.5 Test method for measuring trace metals in electronic grade
water by atomic absorption spectrophotometry;
- GB/T 11446.6 Test method for SiO2 in electronic grade water by
spectrophotometer;
- GB/T 11446.7 Test method for trace anion in electronic grade water by ion
chromatography;
- GB/T 11446.8 Test method for total organic carbon in electronic grade water;
- GB/T 11446.9 Test method for particles in electronic grade water by
instrument;
- GB/T 11446.10 Test method for total bacterial count in electronic grade
water by membrane filters.
This part is part 10 of GB/T 11446.
This part was drafted in accordance with the rules given in GB/T 1.1-2009.
This part replaces GB/T 11446.10-1997 “Test method for total bacterial count in
electronic grade water by membrane filters”.
As compared with GB/T 11446.10-1997, the main changes of this part are as
follows.
- In clause 3 “Terms and definitions”, REMOVE the definition of “sterile
operation”, ADD the definitions of “total bacteria” and “medium” (SEE
clause 3);
- ADD clause 5 “interference factors” (SEE clause 5);
- DELETE clause 10 “Considerations” (SEE clause 10 of the 1997 version).
This part was proposed by the Ministry of Industry and Information Technology
Test method for total bacterial count in
electronic grade water by membrane filters
1 Scope
This part of GB/T 11446 specifies the test method for total bacterial count (live
bacteria) in electronic grade water by membrane filters.
This part applies to the determination of the total bacterial count in EW - I ~ EW
- IV electronic grade water.
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) are applicable to this standard.
GB/T 11446.1-2013 Electronic grade water
GB/T 11446.3-2013 Generic rules for test methods of electronic grade water
3 Terms and definitions
The terms and definitions defined in GB/T 11446.3-2013 and the following terms
and definitions apply to this document.
3.1
Bacterium colony
After a single cell or spore is cultured on a solid medium for a certain period
of time, it grows and multiplies to form a macroscopic microorganism colony,
which is a colony.
3.2
Bacterium culture method
A certain amount of water sample is filtered through an ultrafiltration
membrane, and bacteria remaining on the surface of the ultrafiltration
2 - Filter membrane;
3 - Membrane carrier network;
4 - Suction filter bottle;
5 - Buffer bottle;
6 - Vacuum pump.
Figure 1 -- Schematic diagram of suction filtration system
8 Operation procedures
8.1 Preparation before testing
8.1.1 TURN on the sterile chamber or sterile workbench for 1 h ~ 2 h, then
MAKE it into a stable working state.
8.1.2 Sterilization of vessels. First USE electronic grade EW-I water to wash the
Petri dishes, sampling bottles, and other glassware clean, BAKE it dry, USE
paper to wrap it tightly, PLACE it into a dedicated aluminum vessel, PLACE it
into a thermostat (160 °C ~ 165 °C) to dry and sterilize it or 2 h.
8.1.3 Sterilization of organic filters. BOIL it for 15 minutes in electronic grade
water, DISCARD boiling water, ADD fresh water to boil it, REPEAT it for 3 times,
or otherwise STERILIZE it under UV light for 1 h ~ 2 h.
Note. Pay attention to safety when sterilizing.
8.2 Water sample determination
TAKE 2.0 mL of T.T.C solution, DROP it into 100 mL of medium at the
temperature of 46 °C ± 1 °C, MIX it uniformly in the Petri dish (about 3 mm ~ 4
mm thick), COOL it to prepare for use. SHAKE it uniformly, TAKE appropriate
amount of water sample. 20 mL ~ 100 mL from EW - I ~ EW - III grade water, 1
mL from EW - IV grade water, USE filtration device to filter it, USE 50 mL of
blank water to flush it, EMPTY it. USE tweezers to place the filter membrane on
the prepared medium, LET the membrane surface face upwards, COVER the
lid; after setting, INVERSE the plate and PLACE it into the incubator to incubate
it at 37 °C ± 1 °C for 48 h. TAKE it out, OBSERVE the colonies under a
stereomicroscope and COUNT it. FOLLOW the same procedure to perform a
blank test. DETERMINE each sample for 3 times in a parallel manner, TAKE
the average value.
9 Measurement result calculation
The total bacterial count in water is calculated in accordance with formula (1).
......
| Standard ID | GB/T 11446.10-2013 (GB/T11446.10-2013) | | Description (Translated English) | Test method for total bacterial count in electronic grade water by membrane filters | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | L90 | | Classification of International Standard | 30.030 | | Word Count Estimation | 6,668 | | Older Standard (superseded by this standard) | GB/T 11446.10-1997 | | Quoted Standard | GB 11446.1-2013; GB 11446.3-2013 | | Drafting Organization | MII materials for Quality Supervision and Inspection Center | | Administrative Organization | China Electronics Standardization Institute | | Regulation (derived from) | National Standards Bulletin 2013 No. 27 | | Proposing organization | Ministry of Industry and Information Technology of the People's Republic of China | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the total number of bacteria electronic grade water (live bacteria) membrane culture testing methods. This standard applies to EW-I ~ EW-IV grade electronic grade water measured total bacteria. |
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