GB 1886.305-2020 (GB1886.305-2020) & related versions
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National food safety standard - Food additive - D-xylose
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GB 1886.305-2020
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GB 1886.305-2020
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GB 1886.305-2020
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National food safety standard - Food additive - D-
xylose
ISSUED ON: SEPTEMBER 11, 2020
IMPLEMENTED ON: MARCH 11, 2021
Issued by: National Health Commission of the People's Republic of China;
State Administration for Market Regulation.
National food safety standard - Food additive - D-
xylose
1 Scope
This Standard is applicable to the food additive D-xylose that uses corncob,
wood as raw materials, is processed and produced by processes such as
hydrolysis, decolorization, purification, evaporation, crystallization, and drying
in the presence of sulfuric acid catalyst.
2 Molecular formula, relative molecular mass and
structural formula
2.1 Molecular formula
C5H10O5
2.2 Relative molecular mass
150.13 (according to 2016 international relative atomic mass)
2.3 Structural formula
3 Technical requirements
3.1 Sensory requirements
Annex A
Inspection methods
A.1 General
The reagents and water used in this Standard refer to analytically-pure reagents
and grade 3 water specified in GB/T 6682 when other requirements are not
indicated. The standard solutions used in the test, standard solutions for
impurity determination, preparations and products are prepared in accordance
with GB/T 601, GB/T 602, and GB/T 603 when other requirements are not
specified. The solution used in the test refers to aqueous solution when it is not
specified which solvent is used for preparation.
A.2 Identification test
A.2.1 Reagents and materials
Fehling reagent
A.2.2 Analysis steps
A.2.2.1 Take 1g of sample and dissolve in 20mL of water. Take 2 to 3 drops of
sample aqueous solution and add to 5mL of boiling Fehling reagent. Red
precipitate is formed.
A.2.2.2 In the determination test of D-xylose content, the retention time of the
main peak of the sample solution chromatogram shall be consistent with the
retention time of the main peak of the standard solution chromatogram.
A.3 Determination of D-xylose content
A.3.1 Reagents and materials
A.3.1.1 Water: secondary distilled water or ultrapure water (pass 0.45μm water
system microporous membrane).
A.3.1.2 Xylose standard product: purity ≥99.0%.
A.3.1.3 Xylose standard solution: Use ultrapure water to mix the standard
xylose into 40mg/mL standard solution.
A.3.2 Instruments and equipment
A.3.2.1 High performance liquid chromatograph: equipped with difference
detector and column constant temperature system.
A.3.2.2 Ultrapure water processor.
A.3.2.3 Ultrasonic dissolver.
A.3.2.4 Analytical balance: resolution is 0.0001g.
A.3.2.5 Micro sampler: 10μL.
A.3.3 Reference chromatographic conditions
A.3.3.1 Chromatographic column: Analytical column (300mm×7.8mm)
dedicated to sugar and sugar alcohol with lead-type strong acid cation
exchange resin as a filler, or equivalent chromatographic column.
A.3.3.2 Column temperature: 75°C.
A.3.3.3 Mobile phase: ultrapure water.
A.3.3.4 Flow rate: 0.6mL/min~0.8mL/min.
A.3.3.5 Injection volume: 10μL.
A.3.4 Analysis steps
A.3.4.1 Sample solution preparation
Weigh an appropriate amount of sample (make the xylose content within the
linear range of the standard solution). Use ultrapure water to set volume to
100mL. After shaking well, use 0.45μm membrane to filter. Collect the filtrate as
the sample solution.
A.3.4.2 Determination
A.3.4.2.1 The standard solution is formulated into 6 standard solution series
with different concentrations in the range of 0.4mg/mL~40mg/mL. After injection
separately, Use the concentration of the standard sample versus the peak area
to make a standard curve. The linear correlation coefficient shall be above
0.9990, otherwise the concentration range needs to be adjusted.
A.3.4.2.2 Inject the standard solution and the prepared sample solution
separately. According to the retention time of the standard sample, qualitatively
determine the chromatographic peaks of various sugar components in the
sample. According to the peak area of sample, use external standard method
or peak area normalization method to calculate the mass fraction of various
sugars.
A.3.5 Result calculation
mean.
A.6 Determination of chloride (calculated as Cl)
A.6.1 Reagents and materials
A.6.1.1 Nitric acid solution: 1+9.
A.6.1.2 Silver nitrate solution: 17g/L.
A.6.1.3 Chloride standard solution: 0.05mg/mL.
A.6.2 Instruments and equipment
Nessler colorimetric tube.
A.6.3 Analysis steps
Weigh 1g of sample, to the nearest of 0.1g. Put in Nessler colorimetric tube.
Add 10mLof water to dissolve. Then add 10mL of nitric acid solution and 1mL
of silver nitrate standard solution. Add water to set volume to 25mL. Place in a
dark place for 5min.
Preparation of standard tube: Accurately draw 1.0mL of chloride standard
solution and process the same with the sample tube at the same time.
A.6.4 Result determination
The turbidity of the sample solution is shallower than that of the standard
solution, that is, the chloride content is less than or equal to 0.005%.
A.7 Determination of sulfate (calculated as SO4)
A.7.1 Reagents and materials
A.7.1.1 Hydrochloric acid solution: 1+9.
A.7.1.2 Potassium sulfate standard solution: Weigh 0.181g of potassium sulfate
and place in a 1000mL measuring flask. Add an appropriate amount of water to
dissolve and dilute to the scale. Shake well to obtain (each 1mL is equivalent
to 100μg of SO4).
A.7.1.3 Barium chloride solution: 25%.
A.7.2 Analysis steps
Weigh 4.0g of sample. Add water to dissolve and dilute to about 40mL (if the
solution is alkaline, add hydrochloric acid dropwise to make it neutral). If the
solution is not clear, it shall be filtered. Place in 50mL Nessler colorimetric tube.
......
| Standard ID | GB 1886.305-2020 (GB1886.305-2020) | | Description (Translated English) | National food safety standard - Food additive - D-xylose | | Sector / Industry | National Standard | | Classification of Chinese Standard | X09 | | Word Count Estimation | 9,964 | | Date of Issue | 2020-09-11 | | Date of Implementation | 2021-03-11 | | Regulation (derived from) | National Health Commission Announcement No. 7 (2020) of the State Administration for Market Regulation |
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