GB/T 31402-2023 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 31402-2023 | English | 260 |
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Measurement of antibacterial activity on plastics and other non-porous surfaces
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| GB/T 31402-2015 | English | 125 |
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Plastics -- Measurement of antibacterial activity on plastics surfaces
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GB/T 31402-2023: Measurement of antibacterial activity on plastics and other non-porous surfaces---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT31402-2023
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 83.080.01
CCS G 31
Replacing GB/T 31402-2015
Measurement of Antibacterial Activity on Plastics and Other
Non-Porous Surfaces
(ISO 22196.2011, IDT)
Issued on. OCTOBER 27, 2023
Implemented on. JUNE 1, 2024
Issued by. State Administration for Market Regulation;
Standardization Administration of the People’s Republic of China.
Table of Contents
Foreword... 3
Introduction... 5
1 Scope... 6
2 Normative References... 7
3 Terms and Definitions... 7
4 Materials... 7
4.1 Bacteria to be used for the tests... 7
4.2 Reagents, culture media and solutions... 8
5 Apparatus... 10
6 Sterilization of Apparatus and Storage of Stock Cultures... 11
6.1 Dry-heat sterilization... 11
6.2 High-pressure steam sterilization... 12
6.3 Preparation of glassware... 12
6.4 Maintenance of stock cultures... 12
7 Procedure... 12
7.1 Pre-culture of bacteria... 12
7.2 Preparation of test specimens... 12
7.3 Preparation of test inoculum... 13
7.4 Inoculation of test specimens... 13
7.5 Incubation of the inoculated test specimens... 15
7.6 Recovery of bacteria from test specimens... 15
7.7 Determining the viable bacteria count by the pour plate culture method... 16
8 Expression of Results... 16
8.1 Determination of the number of viable bacteria... 16
8.2 Conditions for a valid test... 17
8.3 Calculation of the antibacterial activity... 17
8.4 Effectiveness of the antibacterial agent... 18
9 Repeatability and Reproducibility... 18
10 Test Report... 18
Annex A (Normative) Quality of Biological Materials... 20
Annex B (Informative) Repeatability and Reproducibility... 21
Annex NA (Informative) Calculation of Antibacterial Rate... 25
Bibliography... 26
Foreword
This Document was drafted as per the rules specified in GB/T 1.1-2020 Directives for
Standardization – Part 1.Rules for the Structure and Drafting of Standardizing Documents.
This Document replaced GB/T 31402-2015 Plastics - Measurement of Antibacterial Activity on
Plastics Surfaces. Compared with GB/T 31402-2015, the major technical changes of this
Document are as follows besides structural adjustments and editorial modifications.
a) Change the "Scope" to expand the scope of application from plastics to plastics and other
non-porous materials; and add descriptions of materials and products not covered by this
Document (see Clause 1 of this Edition; Clause 1 of the 2015 Edition);
b) Change the requirements for the use of "test bacteria" (see 4.1 of this Edition; 4.1 of the
2015 Edition);
c) Change the requirements for cleaning the specimen in "Preparation of specimens" (see
7.2 of this Edition; 7.2 of the 2015 Edition).
This Document equivalently adopted ISO 22196.2011 Measurement of Antibacterial Activity
on Plastics and Other Non-Porous Surfaces.
This Document made the following minimum editorial modifications.
--- Use Method B in the informative reference document GB/T 19275-2003 to replace
Method C in ISO 846.1997.The relevant technical contents of the two documents are
completely consistent (see Clause 1);
--- Domestic strain numbers equivalent to the tested strains are listed in Table 1 and
explained in footnotes;
--- Add Annex NA (informative) "Calculation of antibacterial rate".
Please note some contents of this Document may involve patents. The issuing agency of this
Document shall not identify these patents.
This Document was proposed by China Petroleum and Chemical Industry Federation.
This Document shall be under the jurisdiction of National Technical Committee on Plastics of
Standardization Administration of China (SAC/TC 15).
Drafting organizations of this Document. Institute of Microbiology, Guangdong Academy of
Sciences (Guangdong Detection Center of Microbiology); Guangdong Demay Biology
Technology Co., Ltd.; Guangzhou Research Institute of Synthetic Material Limited Company;
Jinda Nanotechnology (Xiamen) Co., Ltd.; Guangdong Huabiao Testing Center Co., Ltd.;
Chengdu Qinhuan Technology Co., Ltd.; Zhejiang Huiqian Nanotechnology Co., Ltd.; Cobes
Health Care (Hefei) Co., Ltd.; Orinko Advanced Plastics (Shanghai) Co., Ltd.; Shanghai
Guofan New Chemical Material Co., Ltd.; Zhongxing (Guangzhou) Nano Materials Co., Ltd.;
Shanghai Runhe Nanotechnology Co., Ltd.; and Zhejiang Supor Co., Ltd.
Chief drafting staffs of this Document. Xie Xiaobao, Wang Haojiang, Gan Zhihao, Wu Yongxin,
Zhang Yanli, Peng Ruqun, Ma Mei, Wang Fei, Zhao Ziyan, Yan Gaofei, Yu Jiefeng, Chen Xin,
Ma Zhengsheng, Zeng Heping, Tao Zhiqing, and Zhang Shichuan.
This Document was first-time published in 2015; it is first-time revised hereby.
1 Scope
This Document specifies a method of evaluating the antibacterial activity of antibacterial-
treated plastics, and other non-porous, surfaces of products (including intermediate products).
This Document is not intended to be used to evaluate the effects and propagation of bacteria on
non-porous surfaces without antibacterial treatments. If evaluation is required, see Method B
in GB/T 19275-2003[1].
2 Normative References
The provisions in following documents become the essential provisions of this Document
through reference in this Document. For the dated documents, only the versions with the dates
indicated are applicable to this Document; for the undated documents, only the latest version
(including all the amendments) is applicable to this Document.
ISO 7218 Microbiology of food and animal feeding stuffs – General requirements and
guidance for microbiological examinations
3 Terms and Definitions
For the purposes of this document, the following terms and definitions apply.
Ability of an antibacterial agent to inhibit the growth of bacteria on the surface of materials
treated with an antibacterial agent, as determined by the value of the antibacterial activity.
4 Materials
Prepare physiological saline by placing 8.5 g of sodium chloride in 1 000 ml of distilled or
deionized water and mixing to dissolve. Dilute the phosphate buffer solution prepared in 4.2.3.7
with the physiological saline to an 800-fold volume. Sterilize the phosphate-buffered
physiological saline solution by autoclaving (see 6.2). If this solution is not used immediately
after preparation, store it at 5 °C to 10 °C. Phosphate-buffered physiological saline kept for one
month or longer after preparation shall not be used.
5 Apparatus
Unless otherwise specified, use the following apparatus and materials.
6 Sterilization of Apparatus and Storage of Stock Cultures
Stock cultures shall be stored at 5 °C to 10 °C on an appropriate medium and transferred
monthly. After five transfers or if more than one month has passed between transfers, the stock
culture shall be discarded and replaced with a fresh culture obtained from the institute or culture
collection concerned.
7 Procedure
Testing shall be performed on at least three specimens from each treated test material. At least
six specimens of the untreated material are required. Half of the untreated test specimens are
used to measure viable cells immediately after inoculation and half are used to measure viable
cells after incubation for 24 h.
Using a sterile inoculating loop, transfer one loop of the test bacteria, pre-incubated as specified
in 7.1, into a small amount of 1/500 NB prepared in accordance with 4.2.3.2.Ensure that the
test bacteria are evenly dispersed, and estimate the number of bacteria using direct microscopic
observation and a counting chamber or another appropriate method (e.g.
8 Expression of Results
For each test specimen, determine the number of viable bacteria recovered in accordance with
Equation (1).
The value of the antibacterial activity can be used to characterize the effectiveness of an
antibacterial agent.
9 Repeatability and Reproducibility
Repeatability and reproducibility are discussed quantitatively in Annex B.
10 Test Report
The test report shall include the following information.
Annex A
(Normative)
Quality of Biological Materials
The quality of the components used in the inoculum preparations might differ depending on the
source, and this might cause significant variability in results. Therefore, the composition of the
components used needs to be specified.
Annex B
(Informative)
Repeatability and Reproducibility
The content of this annex is based on the results of extensive research conducted to investigate
the repeatability and reproducibility of the results from this method.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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