GB 5413.18-2010 PDF English
US$85.00 · In stock · Download in 9 secondsGB 5413.18-2010: National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5413.18: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5413.18-2010 | English | 85 |
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National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products
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| GB/T 5413.18-1997 | English | 199 |
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Milk powder and formula foods for infant and young children--Determination of vitamin C content
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GB 5413.18-2010: National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5413.18-2010
GB
NATIONAL STANDARD OF
THE PEOPLE’S REPUBLIC OF CHINA
National food safety standard
Determination of vitamin C in foods for infants and
young children, milk and milk products
Issued on. MARCH 26, 2010
Implemented on. JUNE 1, 2010
Issued by. Ministry of Health of PRC
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Principle... 4
4 Reagents and materials... 4
5 Apparatus... 5
6 Analysis procedures... 6
7 Result representation... 7
8 Precision... 8
9 Other... 8
Foreword
This Standard replaces GB/T 5413.18-1997 "Milk powder and formula foods for
infants and young children - Determination of vitamin C content".
Compared with GB/T 5413.18-1997, the main changes of this Standard are as
follows.
- defined the activity unit of enzyme;
- changed the concentration of o-phenylenediamine solution;
- changed the treatment of specimens containing starch;
- added -- the reaction time after adding boric acid-sodium acetate solution;
- added -- the reaction time after adding o-phenylenediamine solution;
This Standard replaces the following previous standards.
- GB 5413-1985, GB/T 5413.18-1997.
National food safety standard
Determination of vitamin C in foods for infants and
young children, milk and milk products
1 Scope
This Standard specifies the determination method of vitamin C in foods for
infants and young children, milk and milk products.
This standard is applicable to the determination of vitamin C in foods for infants
and young children, milk and milk products. The determination result of this
Standard indicates the total content of reduction-type vitamin C and oxidation-
type vitamin C.
2 Normative references
The following referenced documents are indispensable for the application of
this document. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any
amendments) applies.
3 Principle
Vitamin C (ascorbic acid) is oxidized to be dehydroascorbic acid in the presence
of activated carbon. The dehydroascorbic acid reacts with o-phenylenediamine
to generate fluorescent substances. Use fluorescent spectrophotometer to
determine its fluorescence intensity.
4 Reagents and materials
Unless otherwise specified, the reagents used in the method are analytically
pure; the water used is Grade 3 water regulated in GB/T 6682.
4.1 Amylase. enzyme activity is 1.5U/mg; adjust the dosage in accordance
with the activity unit size.
4.2 Metaphosphoric acid-acetic acid solution A. weigh 15 g of metaphosphoric
acid and 40 mL of acetic acid (36%) in 200 mL of water; dissolve and dilute to
500 mL for use.
4.3 Metaphosphoric acid-acetic acid solution B. weigh 15 g of metaphosphoric
acid and 40 mL of acetic acid (36%) in 100 mL of water; dissolve and dilute to
250 mL for use.
4.5 Sodium acetate solution. use water to dissolve 500 g of sodium acetate
trihydrate till it is diluted to 1 L.
4.6 Boric acid-sodium acetate. weigh 3.0 g of boric acid; use the sodium
acetate solution (4.5) to dissolve till it is diluted to 100 mL; prepare when
required.
4.8 Vitamin C standard solution (100 µg/mL). weigh 0.050 g of Vitamin C
standard product; use metaphosphoric acid - acetic acid solution A (4.2) to
dissolve and make constant volume to 50 mL;
5 Apparatus
5.1 Fluorescence spectrophotometer.
5.2 Scale. inductance of 0.1 mg.
5.4 Incubator. 45°C±1°C.
6 Analysis procedures
6.1 Specimen Treatment
6.1.1 Starch-containing specimen. WEIGH about 5 g (accurate to 0.0001 g)
of well mixed or about 20 g (accurate to 0.0001 g) of liquid specimen
(containing about 2 mg of Vitamin C) in a 150 mL erlenmeyer flask.
6.1.2 Starch-free specimen. WEIGH about 5 g of well mixed solid specimen
(accurate to 0.0001 g). USE metaphosphoric acid-acetic acid A (4.2) to dissolve.
MAKE constant volume to 100 mL.
6.2 Preparation of solution for test
6.2.1 TRANSFER the aforementioned specimens (6.1.1, 6.1.2) and Vitamin
C standard solution (4.8) to a 250 mL erlenmeyer flask having about 2 g of
acidic activated carbon (4.4). Severely VIBRATE it. FILTER it (discard about 5
mL of initial filtration) and it shall be the filtration of specimen and standard
solution. Accurately PIPETTE 5.0 mL of the filtration of specimen and standard
solution. Respectively PLACE in the 25 mL and 50 mL flasks having 5.0 mL of
boric acid-sodium acetate solution (4.6). Place for 30 min. USE distilled water
for constant volume. Use it as the blank solution of specimen and standard
solution.
6.3 Determination
6.3.1 Drawing of standard curve
Immediately transfer the standard series of test solution (6.2.4) into the quartz
curette of the fluorescence spectrophotometer. Determine its fluorescence
value when excitation wavelength is 350 nm, emission wavelength is 430 nm.
Standard fluorescent value respectively minus standard blank fluorescence
value as Y-axis; take the corresponding vitamin C mass concentration as X-
coordinate to draw the standard curve.
7 Result representation
The vitamin C content in specimen shall be calculated according to equation
(1).
8 Precision
The absolute difference between two independent determination results
obtained under repeatability conditions shall not exceed 10% of the arithmetic
mean.
9 Other
The detection limit of this Standard is 0.1 mg/100 g.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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