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YC/T 472-2013: Tobacco and tobacco products. Microbiological examination. Enumeration of moulds
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| YC/T 472-2013 | English | 209 |
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Tobacco and tobacco products. Microbiological examination. Enumeration of moulds
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YC/T 472-2013
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Basic data | Standard ID | YC/T 472-2013 (YC/T472-2013) | | Description (Translated English) | Tobacco and tobacco products. Microbiological examination. Enumeration of moulds | | Sector / Industry | Tobacco Industry Standard (Recommended) | | Classification of Chinese Standard | X87 | | Classification of International Standard | 65.160 | | Word Count Estimation | 8,849 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | On the approval of the State Tobacco Monopoly Bureau issued a routine analytical smoking machine - Definitions and standard cigar conditions 20 tobacco industry standard notice; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | State Tobacco Monopoly Administration | | Summary | This standard specifies the tobacco and tobacco products microbiology mold count. This standard applies to tobacco and tobacco products, mold count. | YC/T 472-2013: Tobacco and tobacco products. Microbiological examination. Enumeration of moulds---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tobacco and tobacco products. Microbiological examination. Enumeration of moulds
ICS 65.160
X87
Record Number. 39853-2013
People's Republic of China tobacco industry standards
Tobacco and tobacco products Microbiological examination
Mold count
Issued on. 2013-02-28
2013-03-15 implementation
State Tobacco Monopoly Bureau issued
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This standard was proposed by the State Tobacco Monopoly Bureau.
This standard by the National Standardization Technical Committee on Tobacco Cigarette Technical Committee (SAC/TC144/SC1) centralized.
This standard was drafted. Shanghai Tobacco Group Co., Ltd., Anhui Tobacco Industry Co., Ltd., Yunnan Tobacco Research Institute,
Zhengzhou Tobacco Research Institute, Hongta Tobacco (Group) Co., Ltd., China National Tobacco Quality Supervision and Inspection Center, Hubei tobacco industry limited liability
The company, China National Tobacco Corporation Qingzhou Tobacco Research Institute.
The main drafters of this standard. Lin Huaqing, Qi Dawei, sand cloud Fei Yao Zhongda, Mitch Lee, Xijia Qin, Ma Yongkai, Wanghao Jun, Wu Fengguang,
Fan Yu, Li roots, Zhang province, Su Yang, Chen Huan, Wang Yi, Wu Da, Yao Jianhua, Shu Junsheng, floor Jiaying, Wang Liang, Zeng Jie, Wang Jiale, Tangxing Hong.
Tobacco and tobacco products Microbiological examination
Mold count
1 Scope
This standard specifies the Tobacco and Tobacco Products Microbiological examination mold count.
This standard applies to mold count of tobacco and tobacco products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
According to the physiological characteristics of mold, choose suitable for mold growth and is not suitable for bacterial growth medium was determined by plate count method
Number of mold samples.
4 Equipment and Materials
4.1 Analysis of balance. a sense of the amount of 0.1g.
4.2 incubator. 28 ℃ ± 1 ℃.
4.3 Refrigerator. 2 ℃ ~ 5 ℃.
4.4 slap type homogenizer.
4.5 pressurized steam sterilizer. working pressure up to 0.15MPa.
4.6 Clean Benches.
4.7 Water bath. temperature up to 45 ℃ ~ 65 ℃, temperature control accuracy of ± 1 ℃.
4.8 sterile glass flasks. 250mL, 500mL.
4.9 sterile pipette. 1mL (with 0.01mL scale), 10mL (with 0.1mL scale).
4.10 sterile petri dish. diameter of 90mm.
4.11 sterile tube. 18mm × 180mm.
4.12 sterile kraft paper bags, plastic bags.
4.13 sterile homogenization bags.
4.14 sterile forceps, scissors and so on.
5 medium and reagents
Formulating medium and sterile distilled water used should conform to GB/T 6682 in the three water specifications.
5.1 Modified potato dextrose agar medium. Composition and Method in Appendix A.
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