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SN/T 2472-2010: Protocol of quarantine technique for Japanese encephalitis
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Protocol of quarantine technique for Japanese encephalitis
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SN/T 2472-2010
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Basic data | Standard ID | SN/T 2472-2010 (SN/T2472-2010) | | Description (Translated English) | Protocol of quarantine technique for Japanese encephalitis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 23,279 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Japanese encephalitis virus isolation and identification, PT-PCR method, latex agglutination test, indirect immunofluorescence assay, plaque subtrahend test hemagglutination inhibition test, complement fixation test and other laboratory and quarantine technical specifications. This standard applies to equine and swine Japanese encephalitis diagnosis and quarantine. | SN/T 2472-2010: Protocol of quarantine technique for Japanese encephalitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of quarantine technique for Japanese encephalitis
Exit inspection and quarantine industry standard book People's Republic of China
Japanese encephalitis quarantine Technical Specifications
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Table of Contents
Preface Ⅰ
1 Scope 1
2 Normative references 1
3 1 Overview
4 Diagnosis 1
5 total judgment 15
Appendix A (normative) reagent preparation 16
Appendix B (normative) Preparation of suckling mice infected brain tissue hemagglutinin antigen 19
Foreword
The Standard Appendix A, Appendix B are normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard drafting unit. Jilin People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Jian-Ping Shi, Meng increasing, XIAO Cheng Rui, Spacing Form, Li Shuqing.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Japanese encephalitis quarantine Technical Specifications
1 Scope
This standard specifies the isolation and identification of the Japanese encephalitis virus, RT-PCR method, latex agglutination test, indirect immunofluorescence test,
Plaque reduction assay, hemagglutination inhibition test, complement fixation test laboratories and quarantine technical specifications.
This standard applies to equine and porcine Japanese encephalitis Diagnosis and Quarantine.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Overview
Japanese encephalitis (Janpaneseencephalitis), also known as Japanese encephalitis, called encephalitis, is quoted by the Japanese encephalitis virus
An acute human and animals were suffering from infectious diseases. Mosquitoes for the media. Horse showing symptoms of encephalitis in humans, pigs showed miscarriage, stillbirth and testis
Inflammation, poultry and other livestock was mostly latent infection. There are significant seasonal. The disease is widely distributed, mainly in Asia, to human and animal health
Kang and the national economy caused great harm and loss.
The disease caused by the pathogen is Japanese encephalitis virus (Japaneseencephalitisvirus) belonging to the Flaviviridae family (Flaviviridae),
Having hemagglutination activity could agglutinate chicken, ducks, geese and sheep red blood cells. Virus resistance is not strong on the outside, more sensitive to chemicals commonly used disinfection
Drugs have good inactivation of trypsin, ether, chloroform, are also sensitive.
Mouse is the most commonly used to separate breeding and animal viruses, have a variety of day-old mouse sensitivity, but 1 day to 3 days old
Suckling mice most susceptible. Intracerebral inoculation of mice diseased 2d ~ 4d after onset, showing loose hair, dull and death within 1d ~ 2d.
3 to 4 weeks of age, mice were inoculated with brain 4d ~ 10d after onset. Rats can also be infected with the disease. Guinea pigs, rabbits, chickens are not susceptible. The most suitable virus
In the chick embryo yolk sac breeding, in addition to the virus can fibroblasts, kidney or hamster kidney cells in the chick embryo, vero cells, MDBK cells and
On the proliferation of mosquitoes and disease-producing cells and the formation of plaque, it can be used to breed and titration of the virus.
Japanese encephalitis diagnostic methods include comprehensive clinical diagnosis, etiological diagnosis and serological tests. Virus isolation and identification, the
All sample handling requirements should be guaranteed to operate within the appropriate level of laboratory biosafety; treated samples used containers, goods,
Experimental material shall be valid after disinfection before transportation from the laboratory.
4 Diagnosis
4.1 Clinical comprehensive diagnosis
Japanese encephalitis has obvious seasonal in temperate and sub-tropical regions, centered on the popular late summer, was sporadic occurrence occurred
In young animals, equine encephalitis obvious symptoms and depression, agitation, depressed or paralysis alternating with excitement. Pregnant sows miscarriage,
Boar occur orchitis, taken after the death of the animal's brain cortex, hippocampus and hypothalamus angle for histological examination, found that non-suppurative encephalitis changes can
As a preliminary diagnosis.
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