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Identification of Xanthomonas Oryzae pv. Oryzae, Xanthomonas oryzae pv. Oryzicola
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SN/T 2372-2009
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PDF similar to SN/T 2372-2009
Basic data | Standard ID | SN/T 2372-2009 (SN/T2372-2009) | | Description (Translated English) | Identification of Xanthomonas Oryzae pv. Oryzae, Xanthomonas oryzae pv. Oryzicola | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 13,146 | | Date of Issue | 2009-09-02 | | Date of Implementation | 2010-03-16 | | Regulation (derived from) | Entry-Exit Inspection and Quarantine circular industry standards (2009. 09. 02) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the rice seeds and rice materials oryzae (Xanthomonas oryzae pr. oryzae) and Rice Bacterial Leaf Blight (Xanthomonas oryzae pV. oryzicola) detection method. This standard applies to imports of rice seeds Xanthomonas oryzae and rice bacterial leaf spot pathogen detection. Planting materials and environments oryzae and rice bacterial leaf spot pathogen detection may also refer to this standard. | SN/T 2372-2009: Identification of Xanthomonas Oryzae pv. Oryzae, Xanthomonas oryzae pv. Oryzicola
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Identification of Xanthomonas Oryzae pv.Oryzae, Xanthomonas oryzae pv.Oryzicola
Exit inspection and quarantine industry standard book People's Republic of China
Xanthomonas oryzae, rice bacterial leaf spot pathogen
The detection method
Posted 2009-09-02
2010-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix C of this standard is a normative appendix, Appendix A, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Hunan Exit Inspection and Quarantine is responsible for drafting, animal and plant quarantine Chinese Academy of Inspection and Quarantine Research
The, People's Republic of China participated in the drafting of Xiamen Exit Inspection and Quarantine.
The main drafters of this standard. Zhu Jinguo, water Zhu Fang, Mo Jin, Chen Yun, Zhong Wenying, Zhao Wenjun, Peng Zi.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Xanthomonas oryzae, rice bacterial leaf spot pathogen
The detection method
1 Scope
This standard applies to the import and export of rice seeds Xanthomonas oryzae and rice bacterial leaf spot pathogen detection.
Planting materials and environments Xanthomonas oryzae and rice bacterial leaf spot pathogen detection may also refer to this standard.
Principle 2
2.1 taxonomic status
cause. Both strains belong Prokaryotae (Procaryotae), Proteobacteria (Proteobacteria), gamma-Proteobacteria (Gammapro-
teobacteria), Xanthomonas mesh (Xanthomonadales), Xanthomonas Branch (Xanthomonadaceae), Xanthomonas (Xan-
thomonas).
2.2 Identification of principle
Xanthomonas oryzae and rice bacterial leaf physiological and biochemical differences between bacteria is to detect pathogens important to distinguish two basis (cf.
See Appendix A and Appendix B).
3 equipment and materials
3.1 incubator. 28 ℃ ± 1 ℃.
3.2 Microscope. Lens was 10 × ~ 100 ×.
3.3 Balance. Precision 0.001g.
3.4 autoclave.
3.5 Refrigerator. 0 ℃ ~ 4 ℃.
3.6 homogenizer. 8000r/min ~ 10000r/min.
3.7 sterilized sample preparation equipment. tweezers, scissors, weighing spoon.
3.8 straw. 1mL, sub-scale 0.1mL, 10mL, sub-scale 1mL.
3.9 sterilized dishes. diameter 90mm, bottom flat glass or plastic disposable sterile petri dish.
3.10 sterilized small test tube. 3mm × 50mm.
4 media and reagents
4.1 SPA medium. See Appendix C, Part C. Chapter 1.
0.001% Tween 20- 4.2 phosphate buffer.
4.3 Gram staining reagents.
4.4 flagella staining reagents. See Appendix C, Part C. 2.
4.5 Liquefaction of gelatin medium. See Appendix C, Part C. 3.
4.6 oxidase reagents.
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