SN/T 2344: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2344-2014 | English | 639 |
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Detection and identification of cucumber green mottle mosaic virus
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SN/T 2344-2014
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| SN/T 2344-2009 | English | 439 |
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Cucumber green mottle mosaic virus quarantine and identification methods
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SN/T 2344-2009
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PDF similar to SN/T 2344-2014
Basic data | Standard ID | SN/T 2344-2014 (SN/T2344-2014) | | Description (Translated English) | Detection and identification of cucumber green mottle mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,134 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Older Standard (superseded by this standard) | SN/T 2344-2009 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the molecular biology and immunology methods cucumber green mottle mosaic virus quarantine identified. This standard applies to seed of a plant quarantine and identification possible with cucumber green mottle mosaic virus, seedlin | SN/T 2344-2014: Detection and identification of cucumber green mottle mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cucumber green mottle mosaic virus quarantine and identification methods)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 2344-2009
Cucumber green mottle mosaic virus quarantine and identification methods
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces the SN/T 2344-2009 "cucumber green mottle mosaic virus quarantine and identification methods."
This standard compared with SN/T 2344-2009, the main technical changes are as follows.
Added "real-time quantitative RT-PCR method" and the "rapid immunochromatographic test."
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Beijing People's Republic of China Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Chen Qing, Liang Miao, Huang Feng, Liao Furong, Chen fine red, Chen Yun, Lin Shiming.
This standard replaces the standards previously issued as follows.
--- SN/T 2344-2009.
Cucumber green mottle mosaic virus quarantine and identification methods
1 Scope
This standard specifies the molecular biology and immunology methods cucumber green mottle mosaic virus quarantine identified.
This standard applies to seed of a plant quarantine and identification possible with cucumber green mottle mosaic virus, seedlings and plant products.
2 cucumber green mottle mosaic virus Basic Information
Name. cucumbergreenmottlemosaicvirus
Abbreviation. CGMMV
Taxonomic position. tobacco mosaic virus genus (Tobamovirus) members
CGMMV by mechanical transmission, but also by seed dispersal. Grafting and other farming operations is one of the main factors of disease epidemics in the field,
CGMMV also been Cuscutasubinclusa, hops dodder (C.lupuliformis) and C.campestris mass poisoning, a plant
Once the disease, if not carried out the killing process, soil can also be carriers of the virus, infected soil can also become the source of infection of disease occurrence.
For more information, see Appendix A.
3 PRINCIPLE OF THE METHOD
According to a specific reaction of cucumber green mottle mosaic virus antibody between the blades of melon seeds or ELISA test; the basis of the
Characteristics of the viral genome-specific PCR testing, the results determined by electrophoretic bands size.
4 Equipment and Reagents
4.1 equipment and appliances
Trace Mills, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR instrument, electrophoresis, horizontal electrophoresis tank gel imager,
Desktop high-speed refrigerated centrifuge, water bath, pH meter, pipettes (1000μL, 200μL, 20μL, 2μL), 96 well microtiter plates, mortar or the like; anti
Insect greenhouse.
4.2 Reagents
Reagents and buffers see Appendix B, Appendix C, Appendix D, Appendix E, Appendix F, Appendix G.
Preparation of the test sample 5
5.1 Preparation of seed samples
Picked malformation, immature seeds were sown in sterilized soil, grow to be 3 to 4 leaves of plants will show symptoms numbers do not show symptoms of the plant
Strain grouping (10 per group) and numbered, collected leaves into 2 parts, respectively, based on the need for follow-up tests.
Can also be picked malformation, immature seeds directly enzyme-linked assays and molecular biology.
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