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| SN/T 2342.2-2010 | English | 359 |
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Detection and identification of apple fruit crinkle viroid
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SN/T 2342.2-2010
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PDF similar to SN/T 2342.2-2010
Basic data | Standard ID | SN/T 2342.2-2010 (SN/T2342.2-2010) | | Description (Translated English) | Detection and identification of apple fruit crinkle viroid | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,972 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the wrinkled apple fruit quarantine and identification of the virus. This standard applies to apples with viral entry wrinkled fruit seedlings or other propagating materials gun epidemic and identification. | SN/T 2342.2-2010: Detection and identification of apple fruit crinkle viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of apple fruit crinkle viroid
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Apple fruit crinkle viroid identification of quarantine
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2342 standard is divided into two parts.
--- Apple stem grooving virus quarantine and identification methods;
--- Apple fruit crinkle viroid quarantine and identification methods.
This section SN/T 2342 Part 2.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. China Inspection and Quarantine Science Research Institute.
The main drafters of this section. MATURAL Weimei Sheng, Zhang Yongjiang, Ma Jie, Xiangning, Li Mingfu.
Apple fruit crinkle viroid identification of quarantine
1 Scope
This section SN/T 2342 provisions Apple fruit crinkle viroid quarantine and identification methods.
This section applies to quarantine and identification with Apple fruit crinkle viroid entry seedlings or other propagating material.
Principle 2
Apple fruit crinkle viroid Name. Applefruitcrinkleviroid, Abbreviation. AFCVd. Apple fruit crinkle viroid feature is present standards
The main basis; taxonomic status of such virus, host range, disease symptoms, geographical distribution, transmission, and other genomic features, see Appendix A.
3 instruments, appliances and reagents
3.1 Instrument
3.1.1 PCR amplification.
3.1.2 electrophoresis, electrophoresis tank.
3.1.3 water bath.
3.1.4 High-speed refrigerated centrifuge.
3.1.5 gel imaging system.
3.1.6 oscillator.
3.1.7 fridge.
3.1.8 electronic analytical balance (0.001g).
3.2 Appliances
3.2.1 adjustable pipette (0.1μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 100μL ~ 1000μL).
3.2.2 adjustable pipette tips.
3.2.3 centrifuge tube.
3.2.4 mortar.
3.3 Reagents
3.3.1 RNA extraction buffer
20mmol/L Tris-HCl, pH8.0
10mmol/L disodium ethylenediaminetetraacetate (C10H14N2O8Na2 · 2H2O)
0.8mol/L sodium chloride (NaCl)
1% sodium dodecyl sulfate (SDS)
0.5% diethyl dithiocarbamate (DIEC A)
2% polyvinyl pyrrolidone (PVP, MV24000-40000)
0.5% mercaptoethanol
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