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Quarantine identification of cronartium spp.
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SN/T 2341-2009
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Basic data | Standard ID | SN/T 2341-2009 (SN/T2341-2009) | | Description (Translated English) | Quarantine identification of cronartium spp. | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.100.01 | | Word Count Estimation | 10,186 | | Date of Issue | 2008-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry pine (Pinus Spp.) And other host plants, including timber, wood products, wood packaging and seedling pine blister rust on quarantine and identification of bacteria. This standard applies to pine blister rust occurs from the countries and regions all pine species and other parasitic plants in quarantine pine blister rust pathogen identification. | SN/T 2341-2009: Quarantine identification of cronartium spp.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine identification of cronartium spp.
Exit inspection and quarantine industry standard book People's Republic of China
Pine Blister Rust quarantine and identification methods
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix D of this standard is a normative appendix, Appendix A, Appendix B, Appendix C is informative appendix.
This standard by the National Certification and Accreditation Administration season who will be proposed and managed.
This standard was drafted. People's Republic of China and the People's Republic of China, Shenzhen CIQ Jiangsu Entry-Exit Inspection
Quarantine Bureau.
The main drafters of this standard. Wang Ying, Cheng Yinghui, Zhang Guiming, Conlin, Chen Chi-nan, Yang Weidong, Lou constant wind, Wu Cuiping.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Pine Blister Rust quarantine and identification methods
1 Scope
Blister rust quarantine and identification of bacteria.
This standard applies to all pine species and other host plants from Pine Blister Rust countries and regions in the specimen pine blister rust
Phytophthora identification.
Principle 2
2.1 taxonomic status
2.2 Identification of principle
Pine blister rust is mainly based on morphological characteristics of quarantine identification, while referring to pathogens in the countries and regions (see Appendix A),
Host range (see Appendix B) and symptoms.
3 Instrument appliances
Stereo microscope, microscope, frozen section machine, autoclave, dropper, tweezers, magnifying glass, axes, saws, alcohol lamp, knife, hand
Surgical scissors, measuring slides, cover slips, slides, and other labels.
4 Reagents and Drugs
Ethanol, acetic acid, formalin, distilled water.
5 test methods
5.1 Observation of Symptoms
Observation Immigration pine and other host plants with or without ulcer symptoms, with or without spores heap like. Check whether the plant stems flow fat, swollen skin
Large, yellow-white from rupture and give birth to a crack in the symptoms of orange rust rusty child or yellow rust spores. Autumn at the onset may be leaking drops of honey (for sex
Spores and honey drops of the mixture), after the disappearance of visible drip honey "blood" like spots. On alternate hosts check the back of the blade Have blister-like summer spores heap,
In the summer spores heap or around the organization have teliospore column.
5.2 Morphology
Suspicious tissue sample or coniferous branches cortical tissue samples FAA (formalin 5mL, glacial acetic acid 6mL, 50% alcohol 89mL)
Fixed degreasing 2h ~ 24h after the hand or frozen sections alternate. STEREOLOGY selected slices intact tissue under the microscope slides made in significant
Observation pathogen morphological characteristics (see Appendix C) under the microscope.
6 identifying characteristics
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