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SN/T 2332-2009: Detection of Vibrio cholerae with real-time PCR at forntier ports
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2332-2009 | English | 209 |
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Detection of Vibrio cholerae with real-time PCR at forntier ports
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SN/T 2332-2009
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Basic data | Standard ID | SN/T 2332-2009 (SN/T2332-2009) | | Description (Translated English) | Detection of Vibrio cholerae with real-time PCR at forntier ports | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 07.100.10 | | Word Count Estimation | 8,839 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682; GB 15984; GB 19489; SN/T 1022; SN/T 1239; WS/T 230 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the border port of Vibrio cholerae fluorescent PCR test objects, test procedures and results are reported. This standard applies to Vibrio cholerae O1 strains and strains of Vibrio cholerae 0139 screening test. | SN/T 2332-2009: Detection of Vibrio cholerae with real-time PCR at forntier ports---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Vibrio cholerae with real-time PCR at forntier ports
Exit inspection and quarantine industry standard book People's Republic of China
Vibrio cholerae border crossings fluorescent PCR detection method
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China SZCIQ, People's Republic of China Zhuhai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Zhu Hai, MO Qiuhua, Zhu Yulan, Fan put, Ze, Zhao Fang, Huang Hua, Zheng Xiaoyan.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Vibrio cholerae border crossings fluorescent PCR detection method
1 Scope
This standard specifies the object border crossings Vibrio cholerae fluorescent PCR testing, testing procedures and results reporting.
This standard applies to Vibrio cholerae O1 and O139 strains of Vibrio cholerae strains screening tests.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 15984 cholera diagnostic criteria and principles
GB 19489 General requirements for laboratory biosafety
SN/T 1022 cholera detection in food exports
SN/T 1239 frontier port inspection of cholera
Applications WS/T 230 clinical diagnostic polymerase chain reaction (PCR) technology
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Also known as real-time PCR (real-timePCR), is the use of fluorescent dyes on the basis of ordinary PCR under the excitation light effect released
Fluorescent light changes directly reflect the PCR amplification product of the amount of change of new technology.
3.2
I.e., cycle threshold, means within each reaction tube of the detected fluorescence signal just reaches the set number of cycles experienced by the threshold value.
3.3
Sequence stems from the sequence of the target; molecular beacon probes in the spatial structure of the stem form a cyclic structure wherein the ring is a probe sequence complementary to the target nucleic acid
Complementary sequence-independent configuration; end of the stem is connected to a fluorescent molecule, and the other end connected to a quencher molecule. When no target sequence is present, fluorescent
Optical beacon was stem-loop structure, a fluorescent molecule and quencher molecule is very close to the stem, the fluorescence emitted by fluorescent molecules absorb heat and quencher molecule
Form of energy dissipation, no fluorescence signal detected at this time; when there is the presence of the target sequence, the fluorescence of the beacon probe loop sequence-specific binding to the target sequence
Together, forming duplexes are more stable than the stem-loop structure of molecular beacons, fluorescent molecule and quencher molecule separately, at this time the fluorescence emitted by the fluorescent molecule
Can not be absorbed quencher molecule, the fluorescence can be detected. Therefore, to achieve a cumulative fluorescent signal PCR product formation completely synchronized, thus
Real-time monitoring of PCR amplification.
4 Abbreviations
The following abbreviations apply to this standard.
4.1
Vibrio cholerae.
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