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Import and export of milk and dairy products sulfa drug residues determination method radioreceptor analysis
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SN/T 2312-2009
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Basic data | Standard ID | SN/T 2312-2009 (SN/T2312-2009) | | Description (Translated English) | Import and export of milk and dairy products sulfa drug residues determination method radioreceptor analysis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 14,196 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of milk and dairy products sulfa drug residues in the determination of sample preparation and analysis methods Radioreceptor. This standard applies to milk and milk powder sulfadiazine, sulfamethyldiazine, sulfamethazine, sulfamonomethoxine, between two methoxy pyrimidine sulfonamides, sulfonamide quinoline (mouth evil) morpholine, Sulfamethoxypyridazine, sulfamethoxazole (oral evil) yl, Sulfathiazole, Sulfachloropyridazine, sulfonamides iso (mouth evil) yl, sulfonamide, sulfa pyridine, thiazole sulfamethoxazole, sulfonamides nitrobenzene, sulfaguanidine and sulfa two methoxy pyrimidine residues The screening test. | SN/T 2312-2009: Import and export of milk and dairy products sulfa drug residues determination method radioreceptor analysis
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Import and export of milk and dairy products sulfa drug residues determination method radioreceptor analysis
Exit inspection and quarantine industry standard book People's Republic of China
Import and export milk and dairy products sulfa drug residues
Radio-receptor assay detection method
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Linhai Dan, Lu Qin, Chen snow, Xie Shou new, Raymond, Zouzhi Fei, Zhang dollars.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export milk and dairy products sulfa drug residues
Radio-receptor assay detection method
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor import and export milk and dairy products sulfa drug residues determination.
This standard applies to milk and milk powder room sulfadiazine, sulfamethyldiazine, sulfamethazine, suladimethoxypyrimidine, sulfadiazine
Methoxy pyrimidine, quinoline sulfonamides
Morpholino, sulfa methoxy pyridazine, sulfamethoxazole
Oxazole, thiazole sulfonamides, sulfonamide chloro pyridazine, sulfonamides different
Thiazole, sulfonamide, sulfonamide
Pyridine, thiazole sulfamethoxazole, sulfa nitrobenzene, sulfadoxine and o-methoxy pyrimidine sulfonamides residue screening test.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Sample Preparation
Mixing the original sample was removed from the part of the representative sample, milk powder less than 500g, milk less than 500mL, sharing two charged Jie
Net within the container as a sample, and sealed and labeled. Sample preparation process should prevent sample contamination or microbial or chemical residues occur
Changes matter content.
3.2 Sample preservation
Samples frozen at -18 ℃ below.
4 Detection
4.1 Principle of the method
The competition is based on the detected immune response receptors, [3H] labeled and sulfamethazine residues in samples of sulfa drugs and microorganisms
Competitive binding was specific receptor on the cell. Was measured by liquid scintillation counting of samples [3H] Content count value (cpm), the count value of
In the sample is inversely proportional to the amount of sulfa drug residues.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 CharmⅡ milk sulfonamides assay kit 1).
1) CharmⅡ competitive receptor binding sulfa milk test kit is provided by the trade name of the company's products Charm. This information is given to
Convenient user of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent
product.
4.2.1.1 Negative control milk. Store at 0 ℃ ~ 4 ℃.
4.2.1.2 sulfamethazine or more antibiotics standard mixture (containing sulfamethazine) and stored at 0 ℃ ~ 4 ℃.
4.2.1.3 receptor reagent tablets, -15 ℃ or less to save.
4.2.1.4 [3H] sulfamethazine pharmaceutical tablets labeled, -15 ℃ or less to save.
4.2.2 sulfamethazine standard. purity not less than 99%, CAS number 57-68-1.
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