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Import and export of milk and milk products macrolide drug residue detection methods radioreceptor analysis
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SN/T 2311-2009
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Basic data | Standard ID | SN/T 2311-2009 (SN/T2311-2009) | | Description (Translated English) | Import and export of milk and milk products macrolide drug residue detection methods radioreceptor analysis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 15,182 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of milk and dairy products macrolide drug residue determination of the sample preparation and analytical methods Radioreceptor. This standard applies to milk, milk powder, whey powder erythromycin, tilmicosin, spiramycin, tylosin and lincomycin drug residue screening tests. | SN/T 2311-2009: Import and export of milk and milk products macrolide drug residue detection methods radioreceptor analysis
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Import and export of milk and milk products macrolide drug residue detection methods radioreceptor analysis
Exit inspection and quarantine industry standard book People's Republic of China
Import and export milk and dairy products macrolides residues
Detection method
Radioreceptor assay
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Lu Qin, Xi Jing, Raymond, Zhuliu Ming, Wu Hairong.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export milk and dairy products macrolides residues
Detection method
Radioreceptor assay
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor import and export milk and dairy products macrolide antibiotic residues measured.
This standard applies to milk, milk powder, whey powder erythromycin, tilmicosin, spiramycin, tylosin and lincomycin drug residues
Screening assays.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Sample Preparation
Mixing the original sample was removed from the part of the representative sample, milk powder less than 500g, milk less than 500mL, sharing two charged Jie
Net within the container as a sample, and sealed and labeled. Sample preparation process should prevent sample contamination or microbial or chemical residues occur
Changes matter content.
3.2 Sample preservation
Dry samples were sealed and stored at room temperature, the liquid sample at 0 ℃ ~ 4 ℃ refrigerator to save 5d, or less -15 ℃ can be stored frozen for two months.
4 Detection
4.1 Method summary
Based detection is a competitive receptor immunoreactivity. When the sample contains a macrolide antibiotic, macrolide antibiotic sample
Su residue corresponding receptor binding reagent binding site of the receptor, thereby preventing the [14C] labeled reagent erythromycin receptor sites knot
COOPERATION. Samples containing macrolides higher the content, the more competition binding sites, [14C] erythromycin binding to the less marked, with liquid
Scintillation counter measurement sample of [14C] content cpm value (countperminute, that is, the number of pulses per minute) lower. Samples
Macrolide antibiotic residues is inversely proportional to the amount of cpm value.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 macrolide antibiotics in milk CharmⅡ RIA Kit 1) (MRLsSequentialAssay). pills identified as E.
4.2.1.1 receptor reagent tablets. the following -15 ℃ cryopreservation.
4.2.1.2 [14C] erythromycin pharmaceutical tablets marked. the following -15 ℃ cryopreservation.
1) CharmⅡ macrolide antibiotics in milk competitive receptor binding test kit is provided by the product under the trade name Charm company. Given this
Information in order to facilitate a user of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use
These equivalent.
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