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Β-lactam drug residues detection method radioreceptor analysis in the import and export of milk and dairy products
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SN/T 2310-2009
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Basic data | Standard ID | SN/T 2310-2009 (SN/T2310-2009) | | Description (Translated English) | ��-lactam drug residues detection method radioreceptor analysis in the import and export of milk and dairy products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 14,183 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the milk and dairy ��-lactam drug residue determination of sample preparation and analysis methods Radioreceptor. This standard applies to milk, milk powder, whey powder amoxicillin, penicillin, ampicillin, cefazolin, oxacillin, dicloxacillin, cloxacillin, ceftiofur, cephalexin drug residue screening tests. | SN/T 2310-2009: Β-lactam drug residues detection method radioreceptor analysis in the import and export of milk and dairy products
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Β-lactam drug residues detection method radioreceptor analysis in the import and export of milk and dairy products
Exit inspection and quarantine industry standard book People's Republic of China
Import and export milk and dairy products
The residue detection method β- lactams
Radioreceptor assay
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Lu Qin, Raymond, Zhuliu Ming, Chen Yonghong, Chen light snow.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export milk and dairy products
The residue detection method β- lactams
Radioreceptor assay
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor import and export milk and dairy products within β- lactam drug residues determination.
This standard applies to milk, milk powder, whey powder amoxicillin, penicillin, ampicillin, cefazolin, oxacillin, dicloxacillin,
Cloxacillin, ceftiofur, cephalexin drug residue screening test.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Sample Preparation
Mixing the original sample was removed from the part of the representative sample, milk powder less than 500g, milk less than 500mL, sharing two charged Jie
Net within the container as a sample, and sealed and labeled. Sample preparation process should prevent sample contamination or microbial or chemical residues occur
Changes matter content.
3.2 Sample preservation
Dry samples were sealed and stored at room temperature, the liquid sample refrigerated at 0 ℃ ~ 4 ℃ save the following 5d, -15 ℃ can be stored frozen for two months.
4 Detection
4.1 Method summary
Based detection is a competitive receptor immunoreactivity. When the sample contains β- lactam antibiotics, sample β- lactam anti-
Antibiotic residue in the receptor binding reagent corresponding receptor binding site, thereby preventing the [14C] labeled penicillin G receptor reagent positions
Site binding. Samples β- lactams higher the content, the more competitive binding sites [
14C] labeled penicillin G binding to the less,
Measured by liquid scintillation counting sample [14C] content cpm value (countperminute, that is, the number of pulses per minute) lower. Samples
β- lactam antibiotic residues is inversely proportional to the amount of cpm value.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 milk within β- lactam antibiotics charmⅡ assay kit
1) (MRLSequentialAssay). pills identified as P.
4.2.1.1 receptor reagent tablets. the following -15 ℃ cryopreservation.
4.2.1.2 [14C] labeled pharmaceutical tablets penicillin G. -15 ℃ following cryopreservation.
1) CharmⅡ milk within β- lactam antibiotics competitive receptor binding test kit is provided by Charm products company trade names. Given
This information is for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product has the same effect, will enable
These equivalent products.
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