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SN/T 2301-2009: Rapidly detecting method for dengue virus by real-time fluorescence RT-PCR at frontier port
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| SN/T 2301-2009 | English | 239 |
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Rapidly detecting method for dengue virus by real-time fluorescence RT-PCR at frontier port
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SN/T 2301-2009
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Basic data | Standard ID | SN/T 2301-2009 (SN/T2301-2009) | | Description (Translated English) | Rapidly detecting method for dengue virus by real-time fluorescence RT-PCR at frontier port | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 07.100.01 | | Word Count Estimation | 9,952 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | Human infection pathogenic microorganisms directory (Ministry of Health 2006) | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard applies to the border port of entry and exit of people or mosquitoes carrying dengue virus real-time fluorescent RT-PCR rapid detection, including the detection object, specimen collection and handling, testing procedures, determining and reporting the results. This standard applies to persons or border port of entry and exit of mosquitoes carrying dengue virus laboratory. | SN/T 2301-2009: Rapidly detecting method for dengue virus by real-time fluorescence RT-PCR at frontier port
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Rapidly detecting method for dengue virus by real-time fluorescence RT-PCR at frontier port
Exit inspection and quarantine industry standard book People's Republic of China
Border crossings dengue virus real-time fluorescent RT-PCR
Rapid detection method
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Cheng Kui, Huang Ji City, Hong Ye, Li Xiaobo, Xing Lu Qin, Shi Yong Xia, Zhong Yuqing, phase Dapeng, Gobo rotation, Fei Hu,
Chen Yonghong.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Border crossings dengue virus real-time fluorescent RT-PCR
Rapid detection method
1 Scope
This standard applies to the border port of entry and exit or Mosquitoes carrying dengue virus real-time fluorescent RT-PCR rapid detection, including the detection of
Like, specimen collection and handling, testing procedures, and determines and reports.
This standard applies to the entry-exit personnel or border crossings Mosquitoes carrying dengue virus in laboratory tests.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. Any undated reference documents, the latest versions apply to this standard.
Human infection of pathogenic microorganisms directory (Department of Health 2006)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Dengue virus is a flavivirus genus Flaviviridae, including Ⅰ, Ⅱ, Ⅲ, Ⅳ four serotypes, is enveloped single-strand RNA viruses.
Dengue virus can infect humans and cause dengue fever, severe cases may even cause dengue hemorrhagic fever or dengue shock syndrome. Aedes aegypti and Aedes albopictus
Aedes is the main media dengue virus.
3.2
There are a variety of real-time fluorescent RT-PCR method, this standard uses a T Rao q Man hydrolysis probe method, the principle is the conventional RT-PCR
On the basis of adding a specific fluorescent probe, the probe is a period of oligonucleotides, labeled at both ends of a fluorescent reporter group and a
A fluorescence quenching group, the probe is intact, the fluorescence signal emitted by the reporter group quencher absorption, PCR amplification using enzyme q T Rao
5'-3 'exonuclease activity of the enzyme hydrolysis probes, the fluorescent reporter group and fluorescence quenching group separation, the fluorescence monitoring system can receive
The fluorescence signal, i.e., an amplification of each DNA strand, there is formed a fluorescent molecule, to achieve a PCR product accumulation and the formation of a fluorescent signal
Fully synchronized.
4 Abbreviations
The following abbreviations apply to this standard.
4.1
Reverse transcription polymerase chain reaction.
4.2
Cycle threshold values, each reaction tube fluorescent signal reaches the set threshold number of cycles experienced.
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