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SN/T 2300-2009: Detection of mosquito-borne chikungunya virus at frontier port
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Detection of mosquito-borne chikungunya virus at frontier port
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SN/T 2300-2009
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Basic data | Standard ID | SN/T 2300-2009 (SN/T2300-2009) | | Description (Translated English) | Detection of mosquito-borne chikungunya virus at frontier port | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 07.100.01 | | Word Count Estimation | 10,184 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB 19489 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the border crossings Chikungunya mosquitoes carry the virus detection methods, including specimen collection, processing, testing procedures, determining and reporting the results. This standard applies to border crossings Chikungunya mosquitoes carry the virus laboratory. | SN/T 2300-2009: Detection of mosquito-borne chikungunya virus at frontier port---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of mosquito-borne chikungunya virus at frontier port
Exit inspection and quarantine industry standard book People's Republic of China
Mosquitoes carry the frontier port Chikungunya virus
Detection method
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Huang Ji City, Cheng Kui, Hong Ye, Li Xiaobo, Xing Lu Qin, Shi Yong Xia, Zhong Yuqing, phase Dapeng, Gobo rotation, Fei Hu,
Chen Yonghong.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Mosquitoes carry the frontier port Chikungunya virus
Detection method
1 Scope
This standard specifies the border crossings Mosquitoes carrying the Chikungunya virus detection methods, including sample collection, processing, testing procedures, the results of sentence
Set and reports.
This standard applies to border crossings Mosquitoes carrying the Chikungunya virus laboratory.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. Any undated reference documents, the latest versions apply to this standard.
GB 19489 General requirements for laboratory biosafety
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Chikungunya virus is a single-stranded RNA viruses belonging to the genus virus Togaviridae imperial examination. Viral particles are spherical, the average diameter of 42nm, relative to
Molecular mass of 4.3 × 108, sedimentation coefficient of 46S. The virus can cause human infection chikungunya, the main clinical manifestations were fever, Off
Joint pain, rash and mild bleeding. The disease incubation period 3d ~ 12d, Aedes is the main media.
3.2
Real-time fluorescent RT-PCR method is based on the conventional RT-PCR, to add a specific fluorescent probe. The probe for the period
Oligonucleotides, labeled at both ends of a report group and a quencher group. When the probe is intact, the fluorescence signal emitted by the reporter group
Quencher absorption; PCR amplification using T Rao q enzyme 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that the report fluorophore and quencher
Group separation, whereby fluorescence monitoring system can receive the fluorescence signal. When the fluorescence signal of each reaction tube reaches the threshold set by the
Cycles calendar, expressed in Ct values (cyclethreshold).
4 laboratory bio-safety requirements
4.1 Chikungunya virus infection in culture and animal experiments should be carried out within three biosafety (BSL-3) laboratory.
Operation 4.2 uncultured Chikungunya virus material should be carried out within two Biosafety (BSL-2) laboratory.
4.3 Chikungunya virus inactivation related material and non-infectious material operation can be performed in a biosafety (BSL-1) laboratory.
4.4 Chikungunya virus transport packaging materials classified as A class, UN number UN2814.
4.5 Other requirements carried out according to GB 19489.
5 Key Equipment
The main equipment are as follows.
--- Quantitative PCR instrument;
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