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SN/T 2271-2009: Method of the ploymerase chain reaction for detecting genetically modified components in pimiento
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2271-2009 | English | 189 |
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Method of the ploymerase chain reaction for detecting genetically modified components in pimiento
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SN/T 2271-2009
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Basic data | Standard ID | SN/T 2271-2009 (SN/T2271-2009) | | Description (Translated English) | Method of the ploymerase chain reaction for detecting genetically modified components in pimiento | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X26 | | Classification of International Standard | 67.080 | | Word Count Estimation | 7,744 | | Date of Issue | 2009-02-20 | | Date of Implementation | 2009-09-01 | | Quoted Standard | SN/T 1193; SN/T 1204 | | Regulation (derived from) | National Quality Inspection [2009] No. 67 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the exogenous gene transgenic peppers CaMV 35S, NPT ��, NOS, CMV Qualitative PCR detection methods. This standard applies to transgenic pepper sample screening test for resistance to cucumber mosaic virus in transgenic pepper identification. | SN/T 2271-2009: Method of the ploymerase chain reaction for detecting genetically modified components in pimiento
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Method of the ploymerase chain reaction for detecting genetically modified components in pimiento
Exit inspection and quarantine industry standard book People's Republic of China
Qualitative PCR Detection of Genetically Modified peppers
Posted 2009-02-20
2009-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Committee.
This standard was drafted. People's Republic of China Shandong CIQ.
The main drafters of this standard. Gao Hongwei, Liang beads, with Liu, Wang Yan, YU Li Xin, Cao Zhong Lei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Qualitative PCR Detection of Genetically Modified peppers
1 Scope
This standard specifies the transgenic pepper exogenous gene CaMV35S, NPTⅡ, NOS, CMV qualitative PCR detection method.
This standard applies to transgenic pepper sample screening test for the identification of anti-CMV gene transfer of green pepper.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN/T 1193 genetic testing laboratory technical requirements
SN/T 1204 real-time PCR detection method for genetically modified plants and their processed products
3 Terms, definitions and abbreviations
The following terms, definitions and abbreviations apply to this standard.
3.1 Terms and Definitions
3.1.1
This species does not have the will, from other species of functional DNA sequences, by introducing a variety of means to carry out the species
Expression, in order for the species to obtain new varieties of features.
3.1.2
Gene sequence template first denatured at high temperatures into a single strand, DNA polymerase and under appropriate reaction conditions, depending on the template sequence
Two primers were designed in a period of two complementary sequence of the template DNA strand annealing occurs in conjunction with each other, followed by DNA polymerase
Under catalysis in four deoxyribonucleotides (dNTP) as substrates, primer can be extended, and then repeated denaturation, annealing and extension of this
A cycle in which the amplified gene fragment For amplification was geometrically.
3.2 Acronyms
3.2.1
Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene from a plant chloroplast genome encodes.
3.2.2
Cauliflower mosaic virus 35S promoter.
3.2.3
Neomycin 3'-phosphotransferase.
3.2.4
Nopaline synthase 3 'transcription terminator.
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