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SN/T 0331-2013

Search Result of Chinese Standard: 'SN/T 0331-2013'
Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusRelated Standard
SN/T 0331-2013English369 Add to Cart Days<=3 Method for the inspection of bacillus anthrax in animal by-products for export Valid SN/T 0331-2013
SN/T 0331-2013Chinese16 Add to Cart <=1-day [PDF from Chinese Authority, or Standard Committee, or Publishing House]

   
Detail Information of SN/T 0331-2013; SN/T0331-2013
Description (Translated English): Method for the inspection of bacillus anthrax in animal by-products for export
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B45
Classification of International Standard: 65.020.30
Word Count Estimation: 16,119
Older Standard (superseded by this standard): SN 0331-1994
Quoted Standard: GB 19489
Drafting Organization: Neimenggu entry-exit?examination?and Quarantine Bureau
Administrative Organization: Certification and Accreditation Administration
Regulation (derived from): National quality recognition (2013) 569
Proposing organization: Certification and Accreditation Administration of the People's Republic of China
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Summary: This standard specifies the export tinplate cans boiled bamboo shoots sampling, inspection and test results judgment. This standard applies to a capacity greater than or equal to the export inspection boiled bamboo shoots 9L of tin cans.

SN/T 0331-2013
Method for the inspection of bacillus anthrax in animal by-products for export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Replacing SN 0331-1994
Exports of livestock products in Bacillus anthracis test methods
Methodfortheinspectionofbacilusanthraxinanimalby-productsforexport
Issued on: 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces SN 0331-1994 "export of livestock products in Bacillus anthracis test methods."
This standard compared with SN 0331-1994, major technical changes are as follows:
--- Remove the soybean lectin absorption experiments, phage lysis test, beads and animal experiments;
--- Increased capsular staining, Ascoli reaction and polymerase chain reaction (PCR) test.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted: Inner Mongolia, People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard: Wang Haiyan, Ao Weihua, Zhao Zhiguo, Zhaolin Li, Guo Tie Cheng, Feng Yongsheng.
This standard replaces the standards previously issued as follows:
--- SN 0331-1994.
Exports of livestock products in Bacillus anthracis test methods
1 Scope
This standard specifies the export of livestock anthrax testing.
This standard is applicable to the export mane, wool, cashmere, wild animal fur, raw bone particles and other animal products in Bacillus anthracis test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
3 biosafety
Anthrax is a zoonotic disease, the test environment and execution protection requirements in accordance with GB 19489.
4 media and reagents
4.1 sterile defibrinated sheep blood (or horse blood).
4.2 anthrax precipitin standard antigen.
4.3 anthrax precipitin positive sera.
4.4 anthrax precipitin negative serum.
4.5 0.7% sodium bicarbonate nutrient agar plate: A A.1 in the appendix.
4.6 ordinary nutrient broth: see A.2.
4.7 ordinary nutrient agar plate: see A.3.
4.8 pentamidine and polymyxin agar plates: see A.4.
4.9 PLET agar plates: see A.5.
4.10 basic methylene blue staining solution: see A.6.
4.11 Gram stain solution: see A.7.
4.12 Wash sample solution: see A.8.
4.13 0.5% phenol saline: see A.9.
4.14 Electrophoresis grade agarose powder.
4.15 TaqDNA polymerase: 5U/μL.
4.16 100bpDNAladderMarker (DNA molecular weight standard).
4.17 10-fold concentration TaqDNA polymerase buffer (10 × TapBuffer): 15mmol/LMgC1.
4.18 dNTP mixture: deoxyadenosine triphosphate (dATP), deoxythymidine triphosphate (dTTP), deoxyguanosine triphosphate (dGTP), off
Oxygen triphosphate (dCTP) each 2.5mmol/L.
4.19 50 × TAE electrophoresis buffer: see A.10.
4.20 6 × loading buffer: see A.11.
   
 
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